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Showing: 181 - 210 of 344 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...a unique and powerful high-throughput screen to select variants based on performance metrics specifically... was identified from a high-throughput screen, selected for increased voltage response, brightness, and...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...that there is no universal scoring system for selecting a gRNA, as the method of producing the guide (...phenotype is due to an on-target effect. Conclusions Selection of gRNAs for an experiment needs to balance maximizing...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...mouse embryonic stem cell (ESC) line, enrich and select for cells that had successfully incorporated the...eggs, circumventing the need for enrichment and selection offered by mouse ESCs. For example, we often screen...

  4. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...identify which insertions were positive or negative selected for during the experiment (Sandoval-Villegas et...and host range are important to consider when selecting a transposon for a mutagenesis screen. For example...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...experiments, care still needs to be taken when selecting which particular AAVs to use for your research...’s value greatly. Applying this mantra to AAV selection, there are three important factors related to ...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...marker (Fluorescent protein or selection marker) allowing for selection of the transduced cells. In this...

  7. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...effective and accurate way possible it’s important to select the right test for the job (and, uh, gentle reminder...run it.) You don’t need to be a mathematician to select the right statistical test, just do your homework...

  8. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...skills who fulfill a long-term function. When a select group of people are responsible for a group of ...of criticism or judgment - nobody likes to feel useless, and excess people who are not contributing will...

  10. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...broken down broadly into three main steps: 1) Selection and expression of your opsin of choice in the ...features of each of these steps. Today we’ll discuss selection and expression of your opsin, but keep you eyes...

  11. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have..., with a choice of either hygromycin or Basta selection. Yang Lab Single Transcript Multiplexing Plasmids...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...the cell’s chromosomes. With the addition of a selectable marker, this system allows for the oriP/EBNA1...plasmid can hang around for awhile, even after selection is removed. Yu et al found that ⅔ of subclones...

  13. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...geNorm study (Vandesompele, 2002) will help you select the appropriate reference genes - and it is well...26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation. Biotechniques. 2000 Aug;...

  14. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...Addgene’s data processing needs, our criteria for selecting a new workflow management system, and how Nextflow...

  15. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...name was submitted by more than one person, we selected the finalist based on the first time of submission...

  16. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... the third day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery...

  18. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...                     A post shared by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore...

  19. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...Guide are probably the papers and textbooks. I’ve selected “seminal” studies for various categories, including...

  21. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Simon Moe, from Promega, who wrote a guide to selecting fluorescent dyes and ligands. Illuminating Choices...

  25. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...lab, and one for home. The Addgenies voted, and selected three winners in each category. Congratulations...

  29. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base ...

  30. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Cas9 system to developing an antibiotic that selectively kills only the bacteria you want it to, leaving...
Showing: 181 - 210 of 344 results