...couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork, and competition...will combine all of these. Addgenies have been organizing Halloween festivities for years – searching our...sweet – as sweet as all that Halloween candy you’ll gorge yourself on in the coming days. Hopefully, I’ve ...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...

... Volunteering at a Professional Organization Resources on Addgene.org Check out Career's at Addgene ...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...

... transposons are delivered to cells or a model organism, they insert into the host’s genome. Then, the...Eukaryotic Genomes. Annu Rev Genet 41:331–368. https://doi.org/10.1146/annurev.genet.40.110405.090448 Kumar A ...out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez...Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871 Pray, L. (2008) Transposons... for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084 Additional resources on the...

...untagged counterparts. These findings motivated Georgieva et al. to develop new tags with defined 3D structures...aggregates. Using budding yeast as a model system, Georgieva et al. examined the effects of GFP fusions to .... To prove that inntags are indeed innocuous, Georgieva et al. created tagged versions of the cyclin Cdc28...epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small self-structured...

...planning meetings, and finding the best way to organize your time. At a conference, you could be honing...find yourself asking, "Why doesn't this product/organization/position exist yet?", you are in the early stages... about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring artists who are... our transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career...

.... Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590-023-01689-6 LaFleur, M.W., Nguyen...immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. W., Nguyen...system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources on the...Genome Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions...

...Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki, Z., Rohner...vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita, M., Schütze...all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More resources on the...Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...

...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov, ...In Methods in Enzymology (pp. 181–196). https://doi.org/10.1016/bs.mie.2018.01.022 Additional resources... precise targeted manipulation Resources on Addgene.org Browse all viral vectors available at Addgene...

... Future Outlook. Front Immunol 8:1589. https://doi.org/10.3389/fimmu.2017.01589 Jin B, Odongo S, Radwanska...and Therapeutics. Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al ...SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources on the...Against Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody...

...for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava...Methods & Clinical Development 12:248–265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman...Transduction. Human Gene Therapy 31:499–511 . https://doi.org/10.1089/hum.2020.069  Additional resources on ...parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find AAVs for your experiment...

...process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation. Autophagy...provides limited information about what materials or organelles are being targeted for autophagy. The development...for reporting vacuolar turnover of cytosol and organelles in yeast." Autophagy 4.2 (2008): 205-213. PubMed... sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent...

...the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working with viruses...please email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated... we distribute, please send an email to help@addgene.org. Your questions help us expand and improve our...Expression in Mammals Additional Resources on Addgene.org Overview of Viral Production at Addgene Find...

... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict...Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L,...Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276 Additional resources on the...cell-type expression in retinal cells Resources on Addgene.org Find AAVs for your experiment Browse our AAV...

...Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith O, Tilmans...Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke...Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional resources...viral vectors protocols and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's...

...convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran WF, Lam...severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam...humoral immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional resources... Learn more about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection...

...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun A (2015)...recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein ND, Thie...functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Additional resources...recombinant monoclonal antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene...

...Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (...Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813  Tuson JR, Pascoe EW...Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/10.1177/38.7.2355173  Additional resources on the...Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene   ...

... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...Please send any questions or comments to help@addgene.org. Finally we’d like to thank all those who are...Viral Transductions Additional Resources on Addgene.org Visit the Viral Service Page Check Out Our CRISPR...

...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics...Develops Lab Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On...

...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...

...reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare publications...publications or your dissertation Organize data that you already collected into figures for papers or...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...manuscripts: literature searching, reading, and organizing Tips for writing a scientific review article   ...

... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

...living organisms resulting in the emission of photons. The most familiar bioluminescent organism for most...the second most commonly used luciferase. Other organisms from which luciferases have been cloned from include...  Table 2: Properties of native luciferases Organism Peak emission (nm) Size (kDa) Substrate Cofactors...

...detract from the process being depicted, we can’t forget that those other components are still there in ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...In-exhaustive Chromatin-related genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A ...

... be found on its website (biobuilder.org) or by emailing info@biobuilder.org. Of particular interest this...found through experiential learning. “Students may forget the technical details they hear in their classes...All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly Standards Find Plasmids...

... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation....for Site-Specific Recombination Resources at Addgene.org Browse Drosophila Plasmids ...

.... 2021. https://doi.org/10.1111/gtc.12893, Zhao et al. Nat Comm. 2019. https://doi.org/10.1038/s41467-...Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-x Engineered miniature...Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...