Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 866 results for: FER

Showing: 241 - 280 of 866 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...by Addgenie Rebecca Hutcheson.   References and resources References Banasik, M. B., & McCray, P. B., ...second-generation system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked...then, numerous variants have been developed with different mechanisms to inactivate the integrase.  The IDLV...vectors is random, and the integration site will be different for each transduced cell, there’s no telling which... that of regular lentiviral vectors. The only difference is that you would use the integrase-deficient...that titering these IDLVs may require a slightly different protocol than regular lentiviral vectors. This...quantifying the integrated viral DNA along with a reference cellular gene. When titering IDLVs, there is no...

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ..., protected samples and was compatible with the buffer additive MgCl2 and the crosslinking agent glutaraldehyde...PEs) via engineered virus-like particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein...editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional silencing...Histone tail for Autoinhibition Release of Methyltransferase (CHARM), with several advantages over the ...  Rather than overexpressing an exogenous methyltransferase for epigenetic silencing, which can be toxic...tail to recruit and stimulate the endogenous methyltransferase. The strategy is generalizable to a variety...D3L recruits the endogenous, autoinhibited methyltransferase DNMT3A to the genomic location specified by...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although...response. They generated variants optimized for different applications (v82 has higher affinity for glutamate...Measuring local temperature variations and heat transfer at the subcellular level requires a probe with...By fusing two bright fluorescent proteins with different thermal quenching rates (mNeonGreen and tdTomato...sub-millisecond resolution and sub-degree temperature differences under a variety of imaging conditions (155 μs...fast measurement of temperature and local heat transfer. Image reproduced from Lu et al. 2022, under CC-BY-NC-ND...plasmids here!   Lu K, et al. Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature...

  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...solubility, and thus different reagents are useful for biotinylation in different microenvironments. There...used today in molecular biology for an array of different techniques and protocols. In this post we will...and decarboxylases. These enzymes catalyze the transfer of carboxyl groups to organic acids to produce...relatively small, water soluble molecule that does not interfere with the macromolecules it is added to. Biotin... signal and easier detection. This strategy is referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry...variety of biotinylation reagents available that differ in the functional groups or residues they target...a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan, J. E. “Molecular...

  5. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...scientists have developed and fine tuned many different ways to clone DNA fragments which have provided...more and more common, and for good reason. They offer many advantages over the traditional restriction...with an enzyme master mix, which contains three different enzymes: an exonuclease, which chews back the... this doesn’t allow for mix and match of many different fragments. One way around this is to use a combination...kit you will be using. Some of these products may offer advantages that Gibson assembly does not such as...with a G!) but actually work by significantly different methods. Golden Gate cloning does result in the...from one vector to another. There are lots of different ways to clone these days. Gibson and the other...

  6. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...are communicating across disciplines or doing different tasks. But these challenges can be exaggerated...equal opportunity to contribute. Using video conferencing tools like Zoom or Google Hangouts is a great...great way to include researchers from different locations. Having virtual face-to-face meetings as opposed... collaboration includes scientists working in different time zones, it’s important that everyone can access...project at the International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances...techniques are being performed the same way at different sites. I did a lab exchange as part of the collaboration...been able to do from my home lab. Scientific conferences and workshops are another great place to meet...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...system which has been designed to perform gene transfer in vertebrates. The system is comprised of 2 components...transposase has been discovered in the genomes of many different fish species in a variety of non-functional forms...the lab showed that efficiencies of stable gene transfer using SB100x were comparable to stable transduction... vectors were developed into 16 variants with different selection markers (blasticidin, neomycin, puromycin... each contain two SfiI restriction sites with different overhangs depending upon whether they are on the...only likely to grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping Beauty... Beauty transposase enables robust stable gene transfer in vertebrates. Nat Genet. 2009 Jun;41(6):753-...

  8. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...cells. The replicons of plasmids are generally different from the those used to replicate the host's chromosomal...include ColE1, pMB1 (which comes in a few slightly different but well known derivatives), pSC101, R6K, and ...regulated. Generally, control of replication is referred to as "relaxed" or "stringent" depending on whether...maintains about 20 copies per cell, while pUC – which differs by only two mutations – will produce as many as...considerations. *Requires pir gene for replication (reference). **F1 is a phage-derived ori that allows for...particles. Plasmids with phage-derived ori's are referred to as phagemids.   Other factors that affect...clarified? If so please, post in the comments.  References Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz...

  9. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...out if you want to learn more. References and resources References Balloux, F., & van Dorp, L. (2017...called an envelope. Viruses without an envelope are referred to as naked or non-enveloped, while viruses with...viruses, here’s a quick tip on terminology. “Virus” refers to a single type of virus, not the individual viral...infection — researchers were able to create far safer viral vectors that can be used in BSL-1 or BSL-2...experimentally controlled conditions, making them far safer to work with than virions. The four types of viral...vectors (which many researchers will informally refer to as viruses) are named after the viruses they’...

  10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...of many complex cellular behaviors, such as differentiation or metabolism. Our attempts to manipulate these...cerevisiae with few techniques that are easily transferrable across host species or metabolic pathways. A...system for multidirectional control The CRISPR interference (CRISPRi) model consists of a catalytically .... cerevisiae by using three RNA programs to differentially alter the levels of five genes that encode ...sequentially rerouted the metabolic flux to generate differently pigmented derivatives of violacein. Using dCas9...that will still recognize guide RNA but with a different dCas9 binding module, alternative sets of transcriptional...pharmaceuticals. Follow him on Twitter @AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...activation p300 acetyltransferase dCas9 fused to the catalytic domain of p300 acetyltransferase increases levels...transcription, also includes light-regulated histone methyltransferases and deacetylases. CRISPR and epigenetics ...delivery systems. Transcriptional repression DNA Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited...repression. Created with BioRender.com. DNA Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1...Q147L)-EGFP, a fusion of dCas9 to a small DNA methyltransferase from the prokaryote Mollicutes spiroplasma...Schwiesow contributed to updating this article. References Haldeman JM, Conway AE, Arlotto ME, et al (2018... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...they do arise. Happy CRISPR’ing! References and Resources   References Rabinowitz R, Offen D. Single-Base...cutting to occur. The RNA sequence and PAM site confer specificity of the nuclease; however, many Cas ...effects should impact your experimental design differently. Yes, it is always better to reduce off-target...mutation, off-targets must be quantified slightly differently. For a comprehensive approach, RNA-seq would ...to control for off targets as well as clonal differences. If you are performing CRISPR library screens... as a transfection or transduction control, a different gRNA would have distinct off-target sites, eliminating... there really aren’t any effective off-target reference controls. Off-targets and your experiment Every...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...by Hannah Dotson in Dec. 2022.  References and Resources References Gossen M, Bujard H. Tight control...CMV, EF1A, and SV40, are always active and thus referred to as constitutive promoters. Others are only ...In the presence of tetracycline (Tet), TetR preferentially binds Tet over the TetO elements, allowing ...hear some transcription factor and promoter pairs referred to as binary systems. Binary systems are genetic... One can therefore place GAL4 and GAL80 under different promoters and create sophisticated patterns of...wondering, “why is it necessary to have all 3 of the different binary systems above if they all behave very similarly...s00253-018-9485-4. Epub 2018 Nov 3. PMID: 30392122. Pfeiffer, Barret D., et al. “Refinement of Tools for Targeted...

  14. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...For more context, exposition, and detail, please refer to the paper. Why might one be interested in state...register (Fig. 1C below). If attB-attP pairs from different recombinases are overlapping or nested on a register...that enters distinct states when treated with different combinations and orders of two recombinases. ...leveraging qPCR data from multiple primer pairs, one can infer the predominant state of registers within a sample...strong, directional terminators and promoters with buffer sequences on their 5’ and 3’ ends (for insulation... register. The purpose of this is to prevent interference between the transcribed gene and its 5’ UTR.... in biocomputing and biological circuits.     References 1. N. Roquet et al., Synthetic recombinase-based...

  15. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...in 1983. The differences between gram positive and negative bacteria is due to differences in cell wall...incredibly helpful in visualizing differentiations between bacteria, leading to safer medicine and more successful...minoring in studio arts photography.  References and resources References Silhavy, TJ., Kahne, D. & Walker ...negative and positive bacteria are permeable to different classes of antibiotics. The classification system...

  16. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...the liquid. Molecules absorb different wavelengths of light to different degrees and many molecules have...cuvettes. First you measure the absorbance of the buffer that the DNA is in. This is a “blank” and measures...the ladder. You then run samples of your DNA at different dilutions and quantify based on band intensities...elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for ... Jennifer Tsang...

  17. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... 2019), and different combinations of ITR and capsid serotypes vary in their gene transfer efficiency....DNA molecule as the gene that they package. In a transfer plasmid, the transgene is flanked by two ITRs,...vectors, modifications can drastically affect gene transfer efficiency and transgene expression (Zhou et al...expression can be altered by using ITR sequences from different AAV serotypes that vary in their RBE and T-shaped...restriction digest methods.    Resources and references More resources on the Addgene blog Viral Vectors...Addgene.org Addgene's AVV guide AAV Viral Preps References  Earley, L. F., Conatser, L. M., Lue, V. M., ...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...updated by Jennifer Tsang in August 2020 and by Emily P. Bentley in February 2025. References and Resources...for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end...APOBEC3G displayed less predictable sequence preferences.  The Liu lab also used natural and engineered...generation of base editors. Compared to BE3, BE4 offers a 2.3 fold decrease in C→G and C→A products as ...yielded an editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly more...the development of prime editing, which uses a different mechanism than base editing, has allowed scientists...Resources References Chen, L., Zhu, B., Ru, G., Meng, H., Yan, Y., Hong, M., Zhang, D., Luan, C., Zhang, ...

  19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... the transcript. Different Cas orthologues and subtypes function somewhat differently and can be more ...Transcriptional repression (CRISPRi) in bacteria As RNA interference does not function in bacteria, most efforts ...promoters or direct gene knockout. In contrast, CRISPR offers a much more user-friendly way to modulate gene ...Peters, Oren Rosenberg, and Carol Gross used two different genetic systems, targeting either Gram-negative...bacterial species (Peters et al., 2019). The systems differ on how exactly the CRISPRi locus is introduced ...Clostridium difficile, could be targeted and preferentially depleted from gut. While still far from the...the BE3 that further improves editing rates. References Bikard D, Euler CW, Jiang W, Nussenzweig PM, ...

  20. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ... various AAV serotypes can be used to achieve different patterns of expression, researchers often need...empirically for their own experiments. To this end, we offer control AAV in smaller aliquots of 20ul for serotype.... The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes, as...optogenetics lie in the spatiotemporal control offered by the system that allows for specific manipulation...H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which targets expression...Gordon Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity...dependent version of the DREADD hM3D is now also offered as AAV1 (along with AAV PHP.eB, AAV Retrograde,...

  21. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...new features to the tool. Resources and References References Adler, B., Trinidad, M., Bellieny-Rabelo...solve the problem: CasPEDIA, a new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute...CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be a constantly ...plasmids containing that enzyme, and embedded in the References lists on every page you can find direct links...design, etc.). With this feature, you can compare different Cas enzymes to make sure you are getting the desired...the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler...

  22. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ... scientific conference, whether you are attending in person or monitoring that conference via social media...Fast Company, opportunities to speak at conferences, and offers for postdoc positions. Check out the podcast...write a second. While working on this post, I was offered and accepted a position to work remotely as an ...American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly know how I was selected to participate...s worth a read since many of the pointers are different from the other links shared in the post. And the...

  23. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...and it’s already looking like it’s making a big difference. We can now look at many more transcription factors...Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput. In adapting... this for tagging any protein in all kinds of different assays, not just transcription factors. The second...quite small—20 amino acids—so we don’t think it interferes with the function of the protein. Myers: We’ve...Mendenhall: The benefits are two-fold: CRISPR/Cas9 offers high efficiency of inducing double strand breaks... surprising to us that there aren’t a hundred different versions of this out there. Addgene: Why was it... They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have any additional tips...

  24. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...al. designed plasmids containing Cas9 and two different gRNAs, and electroporated them with an ssODN repair...experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency than...41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair template ...about the creation of “designer babies.” The differences in editing between iPS cells and embryos make...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...embryos to only 60%? Unfortunately, given the differences between CRISPR repair in embryos and iPS models...available with current techniques like PGD.  Reference Ma, Hong, et al. "Correction of a pathogenic gene...

  25. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different plasmid, which may lead... entry vectors (pUC57-L1L4 and pUC57-L3L2) that offer the advantage of using restriction digest cloning...plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric Bimolecular...consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all possible combinations...change in protein conformation in response to light, offers a way to spatially and temporally control cellular... or dark- state of the photoswitchable proteins offers a powerful approach to control protein-protein ...

  26. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...reproducibility as scientists can assemble parts from different labs and experimental systems in their own research...mutants, determined protein expression levels from different promoters, and inhibited gene expression using...contributed by Erin Sanders CreLite, designed by the Eisenhoffer lab, is a new tool that increases temporal and...bringing the two halves of Cre together. Image from Eisenhoffer et al., 2019. This novel tool is practical...organ culture, as well as cell lineage studies. Eisenhoffer and his colleagues evaluated CreLite for cell...eye, muscle and epithelial cells, as well as in different stages of development, ensuring the breadth and...

  27. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ... to what’s happening at different points within the wood. There are different types of cellulose fibers...number of DNA binding proteins with Cas9 guided by different promoters. The challenge in building genetic circuits...getting enough regulatory proteins that don’t interfere with each other. CRISPR is so orthogonal and programmable...then you’d need the ability to turn on genes at different times. It requires more complex synthetic networks...circuits, they are extremely orthogonal. It’s a different perspective and you can layer them for more complicated...for multi-input CRISPR/Cas9 genetic circuits References: Nielsen AA, Voigt CA. Multi-input CRISPR/Cas...

  28. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...numerous variants for the different types of opsins available and their differences and uses have been discussed... the different variants [1, 2, 4]. And, of course, the best way to learn about the many different opsin...about this in detail later). But what are the different steps in an optogenetics experiment and what are...available, instructions for obtaining a material transfer agreement, and list of vendors (such as the vector... core at the University of North Carolina) that offer his constructs. Importantly, control viruses (not... or follow him on Twitter @derekpsimonphd.    References 1. Tye KM, Deisseroth K. Optogenetic investigation...

  29. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...collaboration. The field already has comprehensive reference pages and curated databases for scientists including...discussion on the best strategies and protocols for different experiments.  In this blog post let's take a ... peer-reviewed and assigned a DOI that can be referenced. In addition the data published in microPublications...embryonic genes are individually depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi...RNAi using four-dimensional contrast interference (DIC) microscopy and computer imaging processing. The... back frequently for what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN Project:... biology protocols Read our molecular biology reference ...

  30. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...job hunt, I quickly noticed one of the biggest differences between applying for an academic position versus... phone interview. I also transitioned between different lab groups without having a formal interview process...answer once the interview has started. Instead, I preferred to prepare stories to answer interview questions... an opportunity to demonstrate technical and transferable skills that make you qualified for the position... try and circumvent this, test out the video conferencing software you’ll be using in advance of the interview...interview, if possible. Otherwise, read the video conferencing instructions you received from HR carefully....completing each section of your talk. Some video conferencing software allows participants to “raise” their...

  31. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...remember: Keep Ca+2lm and Ca+2rry on! References and Resources References Nasu Y, Shen Y, Kramer L, Campbell...sensors are commonly used calcium reporters with different kinetic parameters. There are three GCaMP types...and Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally...measurements are more susceptible to artifacts and pH interference, and comparison between experiments can be more...sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity), and is a great FRET... also suitable for in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific...

  32. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...antibody for your application!  References and resources References Lu, Q. L., & Partridge, T. A. (1998... while polyclonal antibodies contain a mix of different isotypes and subclasses. They each have their ...of clones and thus the potential to bind many different sites on the target protein they tend to have ...polyclonal primary antibody contains a mix of different isotypes and subclasses, use a broadly reactive... be worth considering an antibody raised in a different species.      Table 1: Isotype considerations...targets, look for primary antibodies raised in different, and distantly related, species, and secondary...

  33. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...mammalian plasmid here and edit on! References and Resources References Kwart, D., Paquet, D., Teo, S. et...cutting efficiency — although both are of course preferred! When trying to strike that balance, you may wonder...sequence constraints, or at least has looser or different requirements. Luckily, there are some available...necessarily more flexible than NGG; they’re often just different (e.g. NGAG, NGAN, etc.). Additionally, alternative...enzymes certainly add more PAM options, none of them offer a one size fits all solution to the issue. Nor are...al). Additionally, SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver Lab (read...

  34. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...in the body contain the same DNA but have very different structure and function, and can have a specialized...syndrome and Prader–Willi syndrome, which have very different disease phenotypes, are two fascinating examples... copies of chromosome 15 are inactivated, but different phenotypes result depending upon whether the improperly...development and progression of cancer through different mechanisms. New FRET based tools to study epigenetics...restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite sequencing. ...the reporter consistently showed significantly different FRET levels between unmethylated and methylated... and treatment and improving patients lives. References 1. Ong-Abdullah, Meilina, et al. "Loss of Karma...

  35. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...variants—nuclease-competent or nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge... both cutting and regulation of expression at different genetic loci within a single cell. One might imagine...use of “orthogonal” Cas9 proteins derived from different bacterial species, each of which interacts with... (1). Although this is possible, this strategy suffers from several limitations. Perhaps most importantly...expression (6). Altering the length of gRNAs targeting different sites of interest thus represents a straightforward...introduced together with truncated gRNAs targeting a different set of loci to induce simultaneous cutting and...DNA sequence or regulating gene expression.     References 1. Esvelt, Kevin M., et al. "Orthogonal Cas9 ...

  36. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...proteins that confer affinity for a target – and this serotype can be selected to preferentially target your...voltage indicators reviewed.   References and Resources   References Beck C, Zhang D, Gong Y. Enhanced... These types of sensors can have a variety of different components, but all contain a FRET pair, a genetic... genetically encoded component of the system to offer unique advantages depending on your experimental...powerful tool that swaps capsid proteins from different viruses to optimize them for targeting your cell...

  37. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...library protocols you can use as a reference. Table 1: Examples of Different Pooled Libraries at Addgene.  ... plasmids in your library (Are the inserts of different sizes?), and the desired coverage for the library...For this reason, if your library has inserts of different sizes (such as a transcription factor overexpression...your growth time (e.g., 16-18 hours). Finding reference protocols There are many decisions to make when...pDNA Library Amplification protocol are useful references. Skim through our Pooled Library page to find...“one-size-fits-all” protocol! Each library is different, and you should always experimentally test the...

  38. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...you can make an informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein,... we will review what advantages degron tags can offer over other protein control methods and compare the...tools to manipulate protein expression. These tags offer several advantages over the alternative systems ... all the tags reviewed here, the SMASh tag does offer a few other unique features. If your protein is ... fluorescent Halo ligand can be added to media, buffer, etc., which will bind and light up a tagged protein...profile their effectiveness with over a dozen different proteins. In the end, you can’t predict with absolute...

  39. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...antibodies, context is key.    References and Resources References Evan GI, Lewis GK, Ramsay G, Bishop... or the C-terminus, Evan, et al. isolated six different monoclonal antibodies (Evan, et al. 1985). Three... found that the N-terminal half of the antigen differs significantly between human and the other species...-9E10 variable regions and sticking them onto different scaffolds. But over the years, several alternative...

  40. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...Labguru, Quartzy offers software designed with scientists workflow in mind and can offer a one-stop solution...create lists of your favorite protocols for future reference. The exchange currently boasts over 2,500 protocols... is a subscription-based software, Labguru does offer free trials and pricing options for academic labs... limited to within your lab group, Labguru does offer access to protcols from external vendors, such as...orders. Quartzy caters to life science labs and offers the service free of charge to scientists by allowing...101 articles on various plasmid features, and reference tables (i.e. antibiotic concentrations, commonly...
Showing: 241 - 280 of 866 results