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We narrowed to 796 results for: arc

Showing: 301 - 350 of 796 results

  1. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...conference. It was inspiring to see how microbiology research is improving lives worldwide and the presentation...the booth. We were happy to chat with lots of researchers who weren’t aware of Addgene’s plasmid sharing... plasmid tools on the horizon for microbiology research and we’d love to help more microbiologists share...being the Director of the Great Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast, Tim...

  2. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...also bring benefits to researchers conducting basic or early pre-clinical research. However, standard terms...concept of gRNA design is still challenging for researchers using CRISPR, and that standardizing and improving...resources and publications CRISPOR gRNA design tool Researchers at the Keystone meeting had great things to say...

  3. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...tagging method that labels cells with heritable barcodes. Barcodes consist of an 8bp variable region and a 6...Guo, Chuner, et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics...generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in model organisms...

  4. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...partnership with Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent...rather than cyan and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation...function of the protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both...posts about choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org...

  5. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns...sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters... viral protocols and videos Find AAVs for your research ...

  6. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively...believe that these results will be beneficial to the research community.    So, why are we so excited? For starters...inventory and more time providing new materials to the research community. References 1. Coroadinha, A. S., ...

  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...genome and proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful...replication When it comes to replication, viruses tend to march to the beat of their own drum. They have a wide ... eliminate or reduce the risk of infection — researchers were able to create far safer viral vectors that... The four types of viral vectors (which many researchers will informally refer to as viruses) are named...

  8. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...visibly complex - and unique - adult. My initial research directions followed that avenue with projects ...interests and skills than what fits for applied research. I focus in general terms on a couple of very ...teaching – often explaining a technical piece of research to others outside that specialty, who may be other...professional meetings, societies, networks just as do research scientists. There we meet others in the scientific...

  9. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Although plasmids started as a somewhat niche area of research, they are now seen as an ubiquitous tool that ...little bit easier for scientists to conduct their research! Since their discovery in the 1950s, plasmids ...have greatly impacted many areas of biological research and have been key in advancing our knowledge in... Marcy Patrick...

  10. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...downward trend in funding and space in academic research labs has more students looking for other options...perspectives in science and engineering Looking at your research from a broader viewpoint may expose which experiments...extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking a break... skill sets Taking the time to identify and research 2 – 3 potential professions will highlight which...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...also includes clone names, which can allow you to search different providers for the same clone. This can...Antibodies community works to make best practices in research antibody selection and use possible, easy, and...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi....

  12. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...Finally, if you are a fellow researcher interested in adapting your research ideas into hands-on educational...students in functional genomics research Learn how the Arabidopsis research center brings plant science to...

  13. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...development by openly sharing their resources with the research community. To honor their legacy, we launched ...choice to share their fluorescent protein based research. This year, we put out another call for applications...genetic variants, what does that mean? Previous research shows that approximately 75% of all protein-coding...target of the anticoagulant drug warfarin. In past research, several VKOR variants have been found that affect...

  14. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...increasingly more cost effective and precise, researchers are now using it to investigate potential therapeutics...Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat AD using CRISPR/Cas9. They are...correct disease hadn’t yet been realized. The researchers chose to explore its potential in mice with a...known as Crygc. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...

  15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...method. Read on to learn how to use it in your research! The starting point: Ligation-independent cloning...only one type of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... for your experiment. Research applications Gene expression control Researchers are always wanting to ...fact, a choice delivery vector from basic science research all the way to clinical gene therapy. But how ...overexpression of all human transcription factors, barcoded libraries to trace cell lineage, and so much more...

  17. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing...10.3791/54832  Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F (2005) Monoclonal Versus Polyclonal Antibodies...https://doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing...resource on the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies...

  18. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...plasmids into lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are...lentiviral prep here Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns... viral protocols and videos Find AAVs for your research ...

  19. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...expression of recombinant proteins for industrial or research purposes requires stable host cells (the simplest...chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor), that...commercial operators, government agencies, active researchers, as well as fellow iGEM teams. We were the proud...more productive and collaborative environment for research.  We’ve also involved ourselves in extensive outreach...

  20. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...have primarily used Cas9 to knock out genes, researchers at the Genetic Pertubation Platform at the Broad...screens because of its lowered editing rate. This research aimed to increase the potential of Cas12a genetic...

  21. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ... Optogenetics Plasmids for Use in Neuroscience Research Neuroscience covers many subfields When I first...neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect Alzheimer's...projects using biological tools. In addition to the researcher using sequencing technologies to study microbiome...making more AAVs and educational content to help researchers use these great tools. If you’re just getting...

  22. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...Drawing on current questions being asked in MIT’s research labs, BioBuilder inspires students and teachers...malleable curriculum, BioBuilder takes cutting-edge research projects in synthetic biology and transforms them...and watching animations that present a specific research problem and experiment to tackle. Next it’s off...and provides a nationally recognized platform in March to exhibit projects at any stage of completion, ...

  23. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...commonly, antibodies of the IgG isotype are used for research purposes. Isolating polyclonal antibodies from...antibodies are particularly useful for certain research applications. Many secondary antibodies are polyclonal...Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...

  24. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...secondary antibodies would save a good deal of it. Researchers are continuously looking for ways to improve ...providing needed flexibility in assay development. Researchers who understand how and why to use secondary antibodies...

  25. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...things. Now, work from Connie Cepko’s lab allow researchers to activate transcription or Cre recombinase ...activation The project began with a frustration many researchers have experienced: wanting to ask and answer experimental...not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to express a variety of ...and we’re eager to see how you use them in your research!  References 1.Tang, Jonathan C.Y., et al. “A...

  26. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences, I ... is a style match for your needs can take some research.  It helps to spend some time thinking about what... Academic Labor Environment from the Future of Research Symposium Resources on the Addgene Website Check...Recent Job Openings Find Plasmids to Expedite Your Research   ...

  27. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...circuits with functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...which is why MoClo is so attractive! It allows researchers to assemble and test many variables all at once...is postdoc in Peter Schultz’s group at Scripps Research, where he works on genetic code expansion.  References...

  28. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...(Bradbury & Plückthun, 2015a, b). Therefore, researchers from over 100 scientific institutions have proposed...scientists can now improve genetically-encoded research tools, it is shocking that the plasmids and sequences... (2012) Raise standards for preclinical cancer research. Nature 483:531–533 . https://doi.org/10.1038/...Reproducibility: Standardize antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a...

  29. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...required a one-hour presentation about a prior research project. In total, the interviews lasted from ...prepared with questions to ask. With a quick Google search for “questions to ask an interviewer,” you can ... they will ask you to present about your prior research. This is a chance to highlight your prior accomplishments...deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science...

  30. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...removing the wild-type TadA from the complex the researchers developed a second set: The ABE8.x-d version...3.2x higher for positions A3-A4 and A8-A10. The researchers selected 8 of the tested ABE8 constructs (ABE8.8... base editing.  Try ABE8 base editors for your research! Base editing in primary human cells  Cell therapy... of ABE8 for primary human cell editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells...

  31. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical... AAV vectors evade host immunity. While basic research and other ongoing clinical trials support the ...Silva Feelixge. Harshana S De Silva Feelixge is a researcher whose work has focused on gene therapy for viral... She is particularly passionate about HIV cure research and science communication.     References 1. Gaspar...

  32. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...Laboratory. Are you a science student or early career researcher looking to break into science communication? ...non-scientists in an unusual setting: Discuss your latest research in a local pub at a Pint of Science event. Or ...gives training in media skills, writing about your research and residential communication. If science journalism...'d like to directly involve the public in your research projects, you can set up a citizen science project...

  33. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...fluorescent tool with magnets would enable new research and applications that don’t yet exist, but it ... this was even possible. There is a variety of research on whether and how organisms respond to magnetic... non-biological conditions. So, they decided to search for a magnetic response among other proteins that...some hypotheses. They are excited to see other researchers from a range of fields, like quantum chemistry...

  34. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...Culture Re-Amplification Protocol (PDF, 71 KB) Barcode Watermelon Library 5,000,000 30 bp At least... Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR Pooled Libraries 200,000–1,200,000 ...Library 3,548 250 - 10,000 bp, plus a 24 bp barcode 250–1,000 ng Solid Joung et al. Nat Protoc...Pooled Library 80,962 1866 bp, plus a 20 bp barcode 400 ng Solid pCMV-Rep 78/68 SSM Library Amplification...

  35. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...luminescence have been widely used in biological research for a long time, chromoproteins are just now being...history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history. In 1987... can be used, why not add some color into your research? If you have a favorite chromoprotein, either ...Addgene.org Find fluorescent proteins for your research Read our Molecular Biology Reference Find plasmids...

  36. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...to monitoring localization; using FP timers, researchers can look at a single image of a cell and understand... production of the initial FP timer in 2000, researchers have realized how problematic aggregation-prone...expression. In a developing embryo, for example, a researcher could determine whether or not newly synthesized...synthetic biology, FP timers might be able to tell researchers whether old or new cells are better at producing...

  37. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...with any scientific research, choice of a technique often comes down to the researcher’s familiarity with...the nature of the experiment, it is up to the researcher to determine which method is most appropriate...stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...

  38. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...

  39. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...data of their users readily accessible via web searches; likewise, most in-person events and venues will...understanding of their audience demographics. Do your research so you can go to where your audience already is...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com...since we live in the digital age, make sure to research any software you may need: cost or freeware alternatives...

  40. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ... Resources on Addgene.org Search for AAV Vectors to use with your research. Visit Our AAV Guide Page ...a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating... new AAVs can serve as tools for basic science research (neuronal tracing and morphologic studies), as...

  41. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...experiments that work anywhere in the genome. Aneesh's research interests include proteomics, machine learning...Taking a nerdy perspective, you might denote your search as follows: (my_house + coffee) - starbucks See...Data. Quilt is a collaborative database. Aneesh's research interests span machine learning, proteomics, and... on Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find Validated gRNAs...

  42. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...proteins included in different CGBE tools allow the researcher to direct the repair of the abasic site. But ... originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...transversion editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing...existing CGBEs. This distinct approach means researchers can now select the best tool for their application...

  43. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...signal at a point on the axis determined by the researcher, based on their controls. You can usually tell...intimated if you’re struggling to understand how the researchers decided to place their gates on the plots! Flow...gating strategy, you can start to ask why the researchers made the decisions they did in their experiments...vitro chondrocytic differentiation: A stem cell research initiative at the King Abdulaziz University, Kingdom...

  44. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled... pooled libraries and one of our most popular barcoding libraries as pre-made viral preps. If you can’...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...feel more confident incorporating them into your research. And, of course, if you have any questions, don...

  45. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance their production either in the plant...help in screening candidate genes and speeding up research. Certainly, there is still more to transient leaf...your lab, it can become a strong tool for your research. Feel free to add further tips and experiences... in the Lee-Parsons Lab.     References D'Aoust, Marc-André, et al. "Transient expression of antibodies...

  46. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...the College of Life Sciences that same year. Her research is focused on the escape mechanism of genome editing...Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-encoded...professor in Synthetic Biology in 2006. The Yang lab's research is focused on developing microbial genome editing...Sciences in September 2019 as a Ph.D. candidate. Her research focuses mainly on the development and application...

  47. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124...cleavage by Cas9 in living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332...enrichment (TREE) in human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713...the CRISPR guide Find CRISPR plasmids for your research ...

  48. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...users, we just released a major redesign of how search works on our site. For Addgenies, development includes...with our software. When this happens, we need to research and hopefully find a comparable library if it’...our users focus on the fun stuff, accelerating research and discovery. We work with transparency, gladly...the Addgene blog Learn about what went into our search engine update Find all Addgene news Resources ...

  49. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...in neuronal circuits or synaptic remodeling, researchers combine the Cre and FLP recombinases to control...combination of Cre, FLP, and inducible systems gives a researcher exquisite control over expression of multiple...Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how...-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids...

  50. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...
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