...receptor are stained with a fluorescent antibody against the receptor and analyzed on a flow cytometer. ...methods. In direct staining, the primary antibody against a target is conjugated to a fluorochrome. In indirect...

...multiplexing of sgRNAs against unique genomic targets or multiple sgRNAs against the same target to ensure...

...animal, and its B cells will churn out antibodies against the injected protein. Several weeks later, blood...protein. However, each animal will make antibodies against different protein epitopes and so variability between...

... create a thin sheet of excitation light that propagates perpendicular to an imaging objective that collects...excitation light is completely reflected, energy is propagated into the sample via an evanescent wave that only...

...steps and the joined modules cannot be separated again by the same cloning enzymes. MXS (MluI-XhoI-SalI...properties, they generated monoclonal antibodies against them, fused them to GFP, and compared them to popularly...In order to overcome these limitations, Takeharu Nagai and colleagues at Osaka University have developed...

...affordably in bacteria. They are compatible with both agarose and polyacrylamide gels. They are shared through...utilize CRISPR Cas9 systems to defend themselves against bacteriophage infection, but some phages fight ...

...increase the chances of inducing an immune response against the capsids or transgenes. Sensory neurons and ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

...even win a $500 travel prize in our open data extravaganza!  Anyone can submit data from their CLOVER...

...creative scientists will deliver some fabulous photos again this year! Why #DecktheLab? Help us spread some...

... community lab in Cambridge, MA, for hosting our agar art fun!...

... the viral service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our...

...ever before. 29:54 - 31:25 : Nature's defenses against gene drives. 31:26 - 33:08 : Plans for future work...

...this holiday season! It is that time of the year again: miniature #Christmas tree goes up at home!This ...

...the bacteria are inoculated over a quadrant of an agar plate. Then, a stick is dragged through the first...

...was no less busy for the Addgene blog which once again had record readership with over 75,000 views. Changing...

...sophisticated interactions with multiple other proteins. Again, little to no change in cell morphology was observed...

...comments.  Lastly, we are planning to launch an e-magazine this year which would feature articles, posts,...

...immune systems that provide bacteria protection against infectious agents. Several enzymes behind this ...

...the plasmid sharing space, we heard over and over again that the legal documents were one of the biggest...

...plating if your transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’...