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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 331 results for: AGA

Showing: 121 - 140 of 331 results

  1. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...voltage difference across a gel matrix (usually agarose) to pull your negatively charged DNA through the...enzymes you used for cloning. Run your digest on an agarose gel. You should see two bands, one the size of ...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... to screen the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully...every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. 2015. PubMed...sgRNA expression vectors (containing ten guides against each of the 8,158 predicted T. gondii protein-coding...

  3. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...These strategies use multiple, unique antibodies against the same protein, each targeting a different epitope...correlation between the results. Using antibodies against different epitopes on the same target reduces the...

  4. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...the DNA backbone that contains a target sequence against the fluorescent reporter in an inverted repeat ...hairpin dsRNA is produced that contains sequence against your gene(s) of interest and the reporter. This...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...receptor are stained with a fluorescent antibody against the receptor and analyzed on a flow cytometer. ...methods. In direct staining, the primary antibody against a target is conjugated to a fluorochrome. In indirect...

  6. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...animal, and its B cells will churn out antibodies against the injected protein. Several weeks later, blood...protein. However, each animal will make antibodies against different protein epitopes and so variability between...

  8. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ... create a thin sheet of excitation light that propagates perpendicular to an imaging objective that collects...excitation light is completely reflected, energy is propagated into the sample via an evanescent wave that only...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...steps and the joined modules cannot be separated again by the same cloning enzymes. MXS (MluI-XhoI-SalI...properties, they generated monoclonal antibodies against them, fused them to GFP, and compared them to popularly...In order to overcome these limitations, Takeharu Nagai and colleagues at Osaka University have developed...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...affordably in bacteria. They are compatible with both agarose and polyacrylamide gels. They are shared through...utilize CRISPR Cas9 systems to defend themselves against bacteriophage infection, but some phages fight ...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...increase the chances of inducing an immune response against the capsids or transgenes. Sensory neurons and ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

  12. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...even win a $500 travel prize in our open data extravaganza!  Anyone can submit data from their CLOVER...

  13. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...creative scientists will deliver some fabulous photos again this year! Why #DecktheLab? Help us spread some...

  15. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ... the viral service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our...

  19. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...was no less busy for the Addgene blog which once again had record readership with over 75,000 views. Changing...

  20. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...sophisticated interactions with multiple other proteins. Again, little to no change in cell morphology was observed...
Showing: 121 - 140 of 331 results