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We narrowed to 504 results for: CIT

Showing: 121 - 140 of 504 results

  1. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate plasmid yield...ensure proper lysis, but you will still face column capacity limitations since the columns can be “clogged”...magnet as you scale up. Table 1: Standard Prep Kit Capacities* Prep size Amount of culture Approximate ... kit pages as well! Some kits that may not be explicitly labeled as “yeast”, “transfection-grade”, or ...

  2. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...We're excited to roll out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for...standards (* above) will be given a DOI for easy citation by others and, when published, will meet FAIR ...it be nice if those weeks and months of work was cited by others?)  Ready to submit?  Fantastic! The next...

  3. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...hospitalization for gynaecologic disorders” in Ghana. We’re excited to see what impact Dr. Bengyella’s work will have... chimeric cellulases that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass...Seeding Labs’ Instrumental Access program. We are excited to discover what new and creative ideas applicants...

  4. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...scientists using Addgene in their labs. And it has been exciting to learn about all the amazing research being ...neuroscience experiments. Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT...community. The scientists we've spoken with are excited and passionate about making such tools open-access...

  5. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch...importantly, what doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used...its ever-evolving needs. For now, we have many exciting things planned in 2023, from the expansion of ...

  6. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...can now assemble designer PUF domains for RNA specificity engineering. “The RNA binding domain is interesting...changing certain amino acids you can change the specificity,” explained Zhanar Abil of the University of ...sequence. By comparison, Abil explained, the specificity of other RNA binding domains depends on the secondary...

  7. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...introduction to pCri, please see the original publication cited below. pCri plasmids are available either individually...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...fluorescent protein to create three colors. The specificity of these orthologs is key: due to differences...illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential imaging...useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References Multicolor...

  9. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...We’re very excited to announce that we are expanding our viral vector service to include a Packaged on... Packaged on Request viral vector prep.    Excited? So are we! This expansion will give researchers... and educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging...

  10. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...accepted that day and was excited for the start of my co-op in January. This excitement did not come without...hopeful to continue on in the process and I was excited when I received an email to invite me back to conduct...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at...brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term...

  12. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort in the shuttle...chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects....done with CRISPR technology in just 2 years and excitement about how much more there is left to do with ...

  13. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...cell activity, enables researchers to silence or incite neuronal firing and study subsequent effects on...swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups were activated...illuminated. By looking at groups of neurons that elicited similar tail movements, they were able to narrow...

  14. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics...going through the pdfs of the 26,000 publications cited. If the information seems incomplete or unclear,...line distributor. A success all around! If you’re excited about this resource and want to learn more, check...

  15. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...on this topic, the authors state, “Both explicit and implicit aspects of today’s postdoctoral training...need to move around every 3-6 years—a short and exciting ride Complicated matrix structures are common...

  16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless of excitation wavelength... emits at 620 nm. The ratio of signal strength excited at 586 nm to 440 nm serves as a measure of autophagy...

  17. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production...to the research community.    So, why are we so excited? For starters, we believe that this approach can... for a host of other AAV serotypes; it will be exciting to test this approach with different capsids. ...

  18. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging (Andrews et ...recombinant antibodies are derived were tested for specificity in knock-out lines or animals.  Addgene will ... educational materials to the resource. We are excited to work with all of you to make this the best and...

  19. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...regions are unique to each antibody and convey specificity to the target while constant regions are identical...resulting chimeric antibody retains the binding specificity to the original target and can be used with secondary...capabilities this process is frequently used to reduce immunogenicity and thereby enhance the therapeutic potential...

  20. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...slower than fluorescent-based methods, such as excitation of Green Fluorescent Protein (GFP) because of...compared to using a high-intensity laser to rapidly excite GFP. As a result of the different mechanisms to...Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible Subcellular Imaging...
Showing: 121 - 140 of 504 results