... and the linkage of intra- and extracellular environments. Found on nearly every cell in the human body...membrane, physically connecting the extracellular environment and the cytoskeleton. Most integrins have three...responses to mechanical forces in the cell’s microenvironment. The actin cytoskeleton exerts tensile force... by the ligand embedded in the extracellular environment. This further stabilizes the extended open integrin...integrin inhibitors and natural ligands, such as fibronectin, to block receptor function, e.g., cell adhesion...

...reviewer’s concerns, you are making your hypothesis stronger. When you share data at a conference poster session.... It can be extremely toxic in a lab or work environment if left unchecked. Substantive conflict occurs...handy: Empathy: If you often find yourself in confrontational situations with others because you simply cannot...Empathizing with people, especially people with whom you strongly disagree, is a critical component of conflict ...will be considered in the right, and one in the wrong. If you arbitrate, one party might get exactly what...

... increase diversity in the academic research environment. The “how many?” question lies at the base of... fact that training in the academic research environment is setup to prepare trainees for further work...receive as postdocs is not transferrable to other environments. Slide from Paula Stephan’s Talk (All slides...succeed in publishing in the current academic environment. Some great tips from this panel included:  ... the structural problems within the research environment itself. At Addgene we hope that all parties involved...

... the midst of a rapidly changing, globalized environment re-emerging from international financial struggle...but also nurture a supportive and protective environment where individuals are empowered to productively...opportunities that truly exist outside their immediate frontiers. Moreover, sharing a culture/country of origin...work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant expertise and...and entrepreneurial visions thereby opening new frontiers for young talent around the world. The wealth ...

...climate change to study plant and marine biology, environmental microbiology, or biofuel production. There’s...Research requires dedication and effort, so building a strong understanding of what you’re getting yourself into...all just people. I’ve found that in the right environment and with supportive mentors, being open and vulnerable... what I don’t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what...the vital things to look for is a supportive environment—it makes all the difference in a lab experience...

...now available in Addgene’s repository for use in neurons, both in culture and in living brain tissue. “The...explained. “It helps us adapt to influences in our environments. We are able to react and learn. All of this... the LITEs to control gene expression in mouse neurons and in the brains of living mice both quickly and... want to apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making...

...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing...CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry’s lab developed a CRISPR-based...targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit...

...notebooks and binder on your desk. Arrange them in chronological order (or by project, if that works better for...can easily spot the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never...could easily lend one to another labmate? Though electronic lab notebooks (ELNs) have gained traction with...to borrow most frequently. Copy these into an electronic file sharing tool, such as Google Drive or Dropbox...

...Detection of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki... B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before/after application...dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et...

...our lab’s environmental impact, but we can all do more to make research more environmentally friendly.... to address, but is often ignored in the lab environment. I recently attended the Go green! Innovative...institute! What should I do in the lab to help the environment? Well, you could: Collect the NEB and/or Millipore...

...potential as a scientist. But I expected something wrong. My capstone project was based on plasmid cloning...successful clones because the bands were all in the wrong place. Except for one. My manager speculated that...my very own research project! Coming from an environment with limited opportunities and support, puts ...puts into perspective just how much one’s environment changes them. Addgene gave me the chance to become ...

...transcript You can also multiplex gRNAs via a polycistronic transcript. Rather than being transcribed from...option described below, plasmids for making polycistronic gRNAs are also available from the Yang lab for...Plasmids: These plasmids are similar to the Csy4 polycistronic system described above, except that they use...gRNAs are flanked by glycine tRNAs to create polycistronic glycine tRNA-gRNA (PTG) constructs. Eukaryotic... Find plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized...

...refresher if you ever lose track of which acronym is which. Acronym Full name Notes CRISPR terms gRNA... of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary at the end if you...activity, and they are sometimes processed by error-prone non-homologous end joining (NHEJ). To mitigate this...edit. NHEJ Non-Homologous End Joining An error-prone cellular process to repair DSBs. Base Editing ...

...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389...different levels of expression. For example, CAG is a strong promoter that drives very high, ubiquitous expression...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...

... paper in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

...available with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal...group optimized their initial system by using a stronger promoter and different biochemical secondary energy...AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use...

...leaves patients incredibly sensitive to sunlight and prone to developing metastatic melanomas and other cancers...,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012, researchers...sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they now think that...that mutations in the gene may prevent neurons from returning to basal conditions normally after periods...

...improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See ...Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also ...chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab...with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV...

...photos of our pets. #SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing...the COVID-19 pandemic, check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health...home, the STEM career exploration site Inventing Heron has a radio reporter challenge, and code.org has...necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is a challenging ...

... chow, a different water filtration system, environmental stability in an incubator), but there’s a lot...for a transparent and collaborative research environment. Journals as enforcers Currently, most of the... formatting can feel like yet another hurdle in front of publication when all they really want to do is... of incentives. Right now, researchers are not strongly rewarded for being transparent in their communication...