We narrowed to 762 results for: SOM

...destinies” and also suggested some concrete actions that can be taken now to solve some of the more “tractable...finally feels to me like the postdocs are taking some steps to control their own future in science (see...issues in our science training infrastructure for some years.  He spoke eloquently and vehemently about...same time, he pointed out, academic research has some big problems resulting from past practices and mores...unique way of funding basic research at NEB and got some applause when he talked about how he boycotts publishing...

...be integrated into the host genome? Expressed episomally long-term? Expressed short-term? Answering these...achieve different biological outcomes. For example, some viruses integrate into the host genome (lentivirus...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...transposon system, CRISPR targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels...levels. With regard to target cells or organism, some primary cells and differentiated cell types are picky...when it comes to transfection/transduction methods. Some ‘difficult to transfect’ cells lines are amenable...

...colleagues and friends why he would give up,“an excellent—some say “cushy”—tenured faculty position for the grind...reasons to stay and good reasons to go.  Here are some questions and perspectives to consider. What do ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...Better resources available ($)—can outsource a task someone else who can do better, no grant writing Job changes...people) -> How can you make a decision about something like this without sufficient data? Starting to...way to shed light on what paths to pursue. Spend some time really thinking about your motivations and ...

...Homology Directed Repair Addgenie Chari Cortez explains some of the basic science behind homology directed repair... protein production and gives a quick rundown of some of the strains you can use to express your protein...Wyatt and Dale Ramsden from UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by...Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match Made in Heaven... with CRISPR as the cargo. Mary Gearing explains some of the clever ways to successfully combine CRISPR...this post, Addgenie Chari Cortez guides us through some common controls and why you might need them. Happy...

...that motivate his interest in neuroengineering, and some of the optogenetic tools that are still missing.... through optogenetics research Addgene: What are some of the big problems that optogenetic tools can help...human behavior, and maybe we can get a handle on some of these mysteries. Another application is clinical...patients? Over a quarter of a million people have some kind of neural implant already. If we can use light...electricity. If you could selectively let in calcium or some other specific ion that would be interesting. It...Addgene: Do you have any special tips or tricks for someone looking to try these optogenetic tools out for ...

...recombinase-dependent AAV vectors. In this article, we will discuss some of the causes of off-target expression and ways ...This means that all DIO/FLEx AAV vectors will have some level of recombination that can lead to leaky expression...is absent. Additionally, we have experienced that some vectors are much more prone to recombination and...the promoter be thoroughly tested in your cells.  Some promoters, such as CAG are very strong and may require...using DIO/FLEx vectors for off-target expression.  Some classes of neurons transiently express TH during...indicating that there is promoter activity. Additionally, some neurons express low levels of GFAP, which can lead...fair amount of time, and it may be tempting to skip some steps and get to experiments. However, by optimizing...

...Everyone in the room has likely just run in from some other commitment. An announcement of purpose can... will too. Even if you are meeting to discuss something difficult, be excited about the prospect of working...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...checking in on the action items from last meeting. Use some positive peer pressure to make sure everyone completes... your meeting types (see above). We’ve provided some tips above for what works well at Addgene, but meetings... what worked well and ask the meeting leader for some tips. Before long, you will be running collaborative...

... a common big-picture goal. It is worth doing some some serious pre-work to find a lab that will be a ...go about selecting new hires. This is followed by some perhaps surprising, yet important, factors to consider...members to share short-term goals and chat about awesome science. Important factors in the search for a ...of a perfect "fit". How competitive is the lab? Some labs have only one author plus the PI on any paper...Things seem to fall into place frequently and you sometimes feel confident in your abilities. You have intense...

...novice (newb). In this post, I’d like to highlight some of the things that popped out to me as a novice ...the microbiome more often than not). If you’re someone who’s considering getting into neuroscience from...sequences of microbial DNA they all seem to require some mathematical know-how for analysis. If you’re looking... expertise to get into the field, there’s likely some way you can use the tools and skills you’ve developed... - if you’ve got a useful tool, there’s likely someway you can apply it in neuroscience. AAV tools from...the Addgene website and on the Addgene blog to get some ideas for future experiments and to find tools that...

...to share some of the tips and tricks and I’ve learned from numerous scientists at NIDA and some of the ...interest 3) Behavioral experimentation I’ll discuss some of the key features of each of these steps. Today...that have been published at this point.  In brief, some standard opsins (see table above) include Channelrhodopsin2...drive most of the constructs. As an alternative, some constructs have been engineered with a double-floxed... stimulation.  It is also important to note that some promoters are so robust they may actually cause ...optogenetics. In next week’s post we’ll delve into some of the important materials science considerations...

...Congratulations Mark Mellett! Siddharth Prakash: X chromosome genes involved in Turner syndrome Turner syndrome... a developmental disorder where one of the X chromosomes is partially or completely missing in women. ... cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal cell migration...developmental defects. Eight genes from the X chromosome are expressed at significantly reduced levels...reagents will facilitate our aims to correct X chromosome gene dosage in TS cells as a first step to reverse...GNB1 mutations, the lab hopes to capture the signalosome of different receptors upon stimulation. “To ...

...quality of the plasmids we make available and provide some advice on the steps you can take to verify your ...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...Remember, when designing a sequencing primer, you need some space between your primer binding site and the first...feature you want to verify is within this range. Read some of our other blog posts for more tips on sequence...have your own best sequencing practices or maybe some tips on how to get better sequencing reads? Tell...

...applications; however, in some cases, your advisor may choose to allot some if not all grant duties to... should offer in order to build upon and address some, if not all, of the limitations of current methodologies.... Now is the time to make bold statements. While some of the statements may be slightly far reaching, ...items to include for the next application. Although some comments may make it clear that the reviewer misunderstood...comes with continual practice and exposure, here are some additional links that offer more advice on writing...

...plasmid’s page. Sometimes we’re missing a piece of the plasmid There are regions of some plasmids that ...acceptor, contains sequence that is over 80% GC. Some IRES sequences also contain regions that are GC ...assemble correctly. Due to these issues, NGS for some plasmids will not result in one complete, circular...with the heading "Addgene Partial NGS Result." In some cases, there will be more information about our ...make available as part of our OpenBio Repository sometime soon. Stay posted!     References Guerin K, ...

... agar. I’ll also provide you with some tips on how to automate some simple processes in your own lab. ...certainly felt this way at Addgene so we’ve been putting some work into finding robots that’ll take a little labor...automate and optimize more protocols in the future - something that will be key for our future growth. One of...developing ways to prep large quantities of DNA because some countries just can’t receive bacteria through the... can take to automate processes in your own lab. Some things your lab can do to get into the robotics ...

...unfortunate experience of witnessing…nothing. For some unclear reason, cells are not getting transduced...five hail Marys that have helped me troubleshoot some technical obstacles when working with virus: 1. ...diagnostic restriction enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive.... If the virus will be used in a couple of days, some researchers prefer to store freshly harvested virus...If you have low titers (which is unavoidable for some vectors) you can concentrate your virus by ultracentrifugation...fluorescent if they were infected. While there may be some low-level fluorescent protein or resistance marker...

... the ribosome performed in his lab. RNA is a structural and functional component of the ribosome complex...made time to go into the city of Guangzhou and see some of the sites. Most fascinating to me was the outdoor... least I could tell that they were mushrooms. In some cases I couldn’t tell if the bags contained flora...transcripts. I remember when small RNA was just something we ran off the bottom of the gel so it wouldn’...complex and Dr. Steitz quipped that RNA in the ribosome is in a complicated conformation “not like DNA which...

...region of chromosome 15. They both result from the inactivation of genes on one copy of chromosome 15 (the...histone proteins that compacts cellular DNA into chromosomes and reduces the risk of dangerous DNA damage....syndrome). In both cases, the other copy of the chromosome is normally imprinted and silenced, and so the.... In both diseases, genes from both copies of chromosome 15 are inactivated, but different phenotypes ... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...

...startup At some point, our ideas got pretty good. We started doing extracurricular research on some of them...on any challenge and quickly become experts in something, even if that thing is opening a pizza joint. ...start us down the entrepreneurial path. We threw some of our own money at this business and started things... little traction for quite a while, and even had some serious self-confidence issues about continuing ...analyze what we want out of our careers: to build something new and beautiful, to engage with the public in...

...   Both gradients can be informative, so spend some time thinking about which type of gradient can help...to consider changing your incubation temperature. Some proteins aggregate at 95 °C, a common lysis temperature...Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting for your timer... blocking, first check your antibody spec sheet. Some antibodies work better with specific blockers, and...overnight or room temperature for 1–2 hours, there is some evidence that antibodies may need longer to reach... washes — although this can be harsh and inhibit some detection reactions. Like blocking, you don’t want... to be the most straightforward. While there are some things you’ll want to consider (see our “technical...