...Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide AAV Viral Preps References...Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. Savy, A.,...Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288. Shitik, ... modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775. Wilmott...Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513. Zhou, Q....transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345....

...Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand...Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui H, Piganeau...End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin...Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT...nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo...Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog Posts from...

...donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., Hussmann...outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph... Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Nishimasu, H., Ran, ...and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo...RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038/s41586-024-07259-6 Additional Resources... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...

...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. References 1. Roberts.../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

...modulating SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD, Eguia R...Neutralization Assays. Viruses 12:513. https://doi.org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub...SARS-CoV-2 spikes. Science 369:1501–1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam A, Windsor... of evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (...Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 Additional resources on...Browse all COVID-19 blog posts Resources on Addgene.org Find COVID-19 resources on our COVID-19 and ...

... https://wiki.hackerspaces.org/Hackerspaces OpenWorm Project Website: http://www.openworm.org/ Cat Microbiome...1,000 active makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...University at NASA Ames, and an instructor at Biocurious.org, Derek is bringing cutting edge technologies...

...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

...e-mail us at  deposit@addgene.org Special cases Please contact us at deposit@addgene.org or +1 (617)-ADDGENE...plasmids, please let us know by contacting deposit@addgene.org. These deposits are considered large deposits.... Email the completed spreadsheet to deposit@addgene.org along with your GenBank files, plasmid sequences...library with Addgene?" or contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) with additional...to your strain. Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) if you’d like ...after submission, please email us at deposit@addgene.org. Include your deposit number and which data needs...

...proto-oncogene product. Mol Cell Biol 5:3610–3616. https://doi.org/10.1128/mcb.5.12.3610-3616.1985 Munro S, Pelham...Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984.tb02263.x  Munro S, Pelham...chain binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, Pelham HRB... luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner S, Behm...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources...Plasmid-based Recombinant Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene...

... spatial genome organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen, Y., Zhang...biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional Resources... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

...Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J et al. (2020...SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger...Camelid Antibodies. Cell 181:1004–1015.e15 . https://doi.org/10.1016/j.cell.2020.04.031 Zimmermann I, Egloff...proteins. Nature Protocols 15:1707–1741. https://doi.org/10.1038/s41596-020-0304-x Zimmermann I, Egloff ...trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources on the...101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection Browse ...

...1513. doi: https://doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803...cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive...events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ONE-GO biosensors...platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...types, spanning both prokaryotic and eukaryotic organisms. Typically, what system is used is dictated by...Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and ...cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H.,...in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991. Urabe, M., Ding, C.,.... Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089/10430340260355347. Vaughn, J. L., Goodwin... noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077....

...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...PubMed PMID: 10470036. 3. Said, H. M. “Biotin: The forgotten vitamin.” American Journal of Clinical Nutrition...

...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman N. (2012)...Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/   Uhlen M, ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources on the ...The Basics of Western Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies...

...through your organization in this field. Please also enter the billing address for your organization. We will...customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! Today we’...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...

...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

...These molecules occur naturally in bioluminescent organisms, but their real power lies in the clever ways ...Although humans have known about bioluminescent organisms and their characteristic radiance for some time...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first ...be responsible and began studying these marine organisms to find the culprits. He even obtained samples...