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Showing: 121 - 140 of 808 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X,...detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, ...nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico, ...-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois,...Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh,...COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special/COVID-19%20detection...available for COVID-19 research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide...

  2. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm.... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...

  3. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...to me… The problem is that scientists sometimes forget how to communicate about their work with non-academic... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...Experimenting in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN (1981... Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L,...for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark...National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin T, Gordon...National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012...trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources on...articles related to antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's...

  5. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer...possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or requests materials...to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the Golden Stopwatch awardees...to all of the winners so far! Don’t see your organization below? Don’t worry--we check periodically for...transfer offices. Drop us a line at techtransfer@addgene.org. Albert Einstein College of Medicine  Baylor...of commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide AAV Viral Preps References...Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. Savy, A.,...Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288. Shitik, ... modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775. Wilmott...Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513. Zhou, Q....transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345....

  7. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand...Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui H, Piganeau...End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin...Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT...nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo...Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog Posts from...

  8. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. References 1. Roberts.../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., Hussmann...outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph... Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Nishimasu, H., Ran, ...and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo...RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038/s41586-024-07259-6 Additional Resources... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...modulating SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD, Eguia R...Neutralization Assays. Viruses 12:513. https://doi.org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub...SARS-CoV-2 spikes. Science 369:1501–1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam A, Windsor... of evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (...Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 Additional resources on...Browse all COVID-19 blog posts Resources on Addgene.org Find COVID-19 resources on our COVID-19 and ...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ... https://wiki.hackerspaces.org/Hackerspaces OpenWorm Project Website: http://www.openworm.org/ Cat Microbiome...1,000 active makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...University at NASA Ames, and an instructor at Biocurious.org, Derek is bringing cutting edge technologies...

  12. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

  13. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...e-mail us at  deposit@addgene.org Special cases Please contact us at deposit@addgene.org or +1 (617)-ADDGENE...plasmids, please let us know by contacting deposit@addgene.org. These deposits are considered large deposits.... Email the completed spreadsheet to deposit@addgene.org along with your GenBank files, plasmid sequences...library with Addgene?" or contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) with additional...to your strain. Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) if you’d like ...after submission, please email us at deposit@addgene.org. Include your deposit number and which data needs...

  14. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...1513. doi: https://doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803...cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive...events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ONE-GO biosensors...platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...types, spanning both prokaryotic and eukaryotic organisms. Typically, what system is used is dictated by...Titering Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and ...cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H.,...in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991. Urabe, M., Ding, C.,.... Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089/10430340260355347. Vaughn, J. L., Goodwin... noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077....

  16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ... spatial genome organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen, Y., Zhang...biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional Resources... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

  17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J et al. (2020...SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger...Camelid Antibodies. Cell 181:1004–1015.e15 . https://doi.org/10.1016/j.cell.2020.04.031 Zimmermann I, Egloff...proteins. Nature Protocols 15:1707–1741. https://doi.org/10.1038/s41596-020-0304-x Zimmermann I, Egloff ...trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources on the...101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection Browse ...

  18. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...proto-oncogene product. Mol Cell Biol 5:3610–3616. https://doi.org/10.1128/mcb.5.12.3610-3616.1985 Munro S, Pelham...Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984.tb02263.x  Munro S, Pelham...chain binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, Pelham HRB... luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner S, Behm...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources...Plasmid-based Recombinant Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene...

  19. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ...handbooks/Springer protocols (pp. 245–260). https://doi.org/10.1007/978-1-0716-4358-7_20  Karmakar, S., Panda...Biotechnology Journal, 22(10), 2950–2953. https://doi.org/10.1111/pbi.14416  Maher, M. F., Nasti, R. A.,...meristems. Nature Biotechnology, 38(1), 84–89. https://doi.org/10.1038/s41587-019-0337-2  Molla, K. A., Sretenovic...editors. Nature Plants, 7(9), 1166–1187. https://doi.org/10.1038/s41477-021-00991-1  Qiao, J., Zang, Y....virus-based delivery in wheat. Nature Plants. https://doi.org/10.1038/s41477-025-02023-8    Additional Resources... in Popular Culture Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and ...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...Analytical Chemistry, 83(12), 4327–4341. https://doi.org/10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson...Academy of Sciences, 74(12), 5463–5467. https://doi.org/10.1073/pnas.74.12.5463  Shendure, J., & Ji, H....Nature Biotechnology, 26(10), 1135–1145. https://doi.org/10.1038/nbt1486  Slatko, B. E., Gardner, A. F.,...Protocols in Molecular Biology, 122(1). https://doi.org/10.1002/cpmb.59  Wang, Y., Zhao, Y., Bollas, A....Nature Biotechnology, 39(11), 1348–1365. https://doi.org/10.1038/s41587-021-01108-x Additional Resources...Plasmid Quality Control Additional Resources on addgene.org Molecular Biology Reference Sequence Analyzer...
Showing: 121 - 140 of 808 results