... of cells as starting material. Therefore, cell types that are of low abundance are not particularly well... controls, multiple replicates and several cell types can strengthen your results. Enrichment or depletion... bit about how CRISPR/Cas9 can be used in other types of experiments, check out our CRISPR Plasmids and...

...in Luxturna™, can efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally...naturally occurring and engineered AAV serotypes that further expands the range of AAV properties is available...available. While only natural serotypes are currently used in clinical trials, a variety of engineered ones...

...proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or... For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium strains see Weigel and Glazebrook...occurs in a coding region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate...

...strategies available for assembling these modular types of constructs, and different methods are suited ...specific restriction enzymes used affect what general types of modules can be assembled. For example, because... MXS-chaining method can be used to build these types of constructs robustly, quickly, and simply. We ...

...majority of cellular repair events will generate these types of mutations, making it an ‘easy’ editing job to... DNA repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly...about optimizing this distance. Homology arms and types of donors To make sure your donor molecule is used...

...persistent transgene expression in certain cell types (Tatsis et al., 2007), potentially because some ...Addgene’s repository is smaller than for other vector types, we hope that this introduction gives a sense of...Production and Troubleshooting Viral Vectors 101: Types of Viruses...

...on the search page to quickly jump to different types of items: Did you notice the “Filter By…” options...The search bar will suggest direct links to other types of searches including depositing labs and institutions...can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of...

... multi-gene knockouts in a wide variety of cell types, gene editing using homology directed repair (HDR...internalization of Cas9-gRNA RNPs by specific cell types (Rouet et al. 2018). This was accomplished using...additional factors to take into account with these types of experiments. One important consideration is that...

...groups and departments. You don’t have to get these types of projects and groups going alone - if you are ... discipline. Getting companies to come to these types of events can be very tough. First I needed to find...growth through my interactions with many different types of people. As mentioned in the beginning, the ability...

...revolutionize neuroscience Manipulation of specific cell types in neuronal networks Double inverted vectors expressing...only means to target and manipulate specific cell types (populations) within neuronal networks in living...Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal...

...otherwise normal cell types are produced, rather than novel, abnormal cell types. Sequencing-based lineage...spending months generating mutants. Unexpectedly, phenotypes sometimes differ depending on whether a gene ...

...cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia...applied to the differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes...

...repressing target genes in a variety of species and cell types can be found on Addgene’s website. Activating target...activating target genes in a variety of species and cell types can be found on Addgene’s website. Fig. 3...optimized for expression in different species and cell types including (but not limited to) bacteria, yeast, ...

...surface, expanding the range of targetable cell types. DIRECTED is compatible with fusogens from different...such as lentivirus and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible...Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology...

...Pooled libraries Scientists have deposited many types of pooled libraries here at Addgene, including cDNA...Addgene are lentiviral pooled libraries, though other types exist.  When you buy a pooled library from Addgene...Spinfection transduction efficiency can vary across cell types and generally requires optimization of many variables...

... a gene in a living animal across multiple cell types using a mouse model for a human neurological disease...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed... improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002). Neurology 92 (15 Supplement...

...expression at certain loci to create changes in phenotypes. For example, CRISPR activation has been used...and Gersbach, 2015; Zetsche et al., 2015). These types of CRISPR activation are also reversible: once the...et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post covering...

...There are many, many different types of experiments carried out by scientists every day. Although the...plasmids would address and answer these questions. Types of control plasmids Part of planning your experiment...expressing the independent variable. There are two types of replicate controls: technical and biological....

...require interaction with the data authors. In some types of research, raw data requires huge amounts of storage...calling it a “sisyphean task” given the endless types of data). Rather, PLOS appears to leave the scope...provide data in formats exported to common file types? And how long should data be archived? Simplifying...

...Because transcription machinery differs between cell types or organisms, promoters must be similarly variable...derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters...an enhancer region. Can be silenced in some cell types. EF1a General expression mRNA Strong mammalian...