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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 121 - 140 of 628 results

  2. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway Clone Collection...cases arise and millions of people die from cancer-related complications each year. Despite these grim statistics...making the results of any such study as clinically relevant as possible. Connecting the research community... initiative to curate our collection of cancer-related plasmids such that each is linked to a known major...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ... genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into AAV when you add in a gRNA and regulatory...

  4. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

  5. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... other Cas effectors can’t, while maintaining relatively high efficiency. These alterations are achieved...inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding...

  6. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    .... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...low rate, there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...

  9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... all the proteins in the class that showed the relative sizes and the conserved domains. This information... maybe you’re researching a topic for which no relevant or recent review exists. Once you pick a topic...format also has the benefit of exposing you to relevant researchers in the field. Finally, you can sound...from start to finish. Find the information that’s relevant to you, note it, and move on. At the beginning...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise... co-transfection of these plasmids, 293T cells released transgene-containing lentiviral particles into...

  16. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...and purification An affinity tag, generally a relatively small sequence of amino acids, is basically a... Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used ...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization...western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires low pH...  0.8 Affinity and Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility...

  17. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...plasmid is packaged for viral delivery, or the relative expression levels between two or more genes is...the same mRNA. The term "self-cleaving" is not entirely accurate, as these peptides are thought to function... plasmids listed in the table above (and their related plasmids) are designed to have one or more of the...fluorescent reporter for easy detection.5  Just released: Addgene's Plasmids 101 eBook And now for some...

  18. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...could be a matter of timing, as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of...editor of Addgene’s eBooks — including the recently released Viral Vectors 101 2nd edition — I was incredibly...lessons learned from our plasmid lab to develop a reliable and robust high quality viral vector service, ...vector service because we realized that having reliable ready-to-use vectors made from plasmids in the...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...are used to show frequency of events, instead of relying on clustered dots. In a contour plot, each line...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...

  20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...L-arabinose) without signs of toxicity or burden relative to a fluorescent protein control. We also added...to produce GVs in their own lab using the most reliable method, which is the transient cotransfection ...
Showing: 121 - 140 of 628 results