...18(2), 67–83. https://doi.org/10.1038/s41579-019-0299-x Resources on Addgene.org CRISPR software and...information on Class 2 Cas enzymes. The information is organized in wiki format and includes biochemical properties...Biochemistry, Biophysics, and Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I...

...your current organization but also important to build relationships outside your organization. The mentor...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

...Antibody Fragments. Antibodies 8(2):28. https://doi.org/10.3390/antib8020028    Wessel GM, McClay DR (1986...Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe...microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301  Additional resources... the Mouse on Mouse Mess in IHC Resources on Addgene.org  Browse ready-to-use antibodies at Addgene...

...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...first step to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...found here. Finally, you will want to have a well-organized database of expected sequences, either for the...

...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...and for registration please go to http://futureofresearch.org/boston/. The Future of Research Symposium...at the Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic ...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

... 27649177. PubMed Central PMCID: PMC5038762. 3. George Abraham, Bobin., et al. "Fluorescent protein based...physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein. Overcoming current...PubMed PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...

... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...

...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...1563. doi: 10.3390/ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook...

...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: ...

...lac operon assembly deposited by NEB – and don’t forget to ask DAD for some help! References Potapov,...to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.8b00333 Pryor, J. M., Potapov...ACS Synthetic Biology, 11(6), 2036–2042. https://doi.org/10.1021/acssynbio.1c00525 Pryor, J. M., Potapov...assembly design. PLOS ONE, 15(9), e0238592. https://doi.org/10.1371/journal.pone.0238592    ...

...2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science. 2021. https://doi.org/10.1126/science.abf9302... Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289     Novel protein cage...pathways.   Cheah et al., bioRxiv 2021. https://doi.org/10.1101/2021.01.30.428974   The CRISPR corner...

...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...

...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...for Your Experiment Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages...

...Resources: http://openspim.org/Welcome_to_the_OpenSPIM_Wiki http://www.janelia.org/lab/keller-lab ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

...a co-founder of the BioBricks Foundation, an organization working to advance biological engineering openly... technical challenge. We have a strategy that organizes how we start the work, and it begins with the ...which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about logic and ... Kendall Morgan...

... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...blog posts about career readiness Resources at Addgene.org Find careers at Addgene Watch out career videos...

... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...Our Microbiology Pages Additional Resouces on Addgene.org Check Out Our Microbiology Pages ...

... Sanchez MI et al., Nat methods. 2019. https://doi.org/10.1038/s41592-019-0665-7 Optimizing fluorescent... Chertkova, AO, et al., bioRxiv. 2020. https://doi.org/10.1101/160374 Nanobodies- there's a simple method...Iwaki et al., Protein Expr Purif, 2020. https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed CRISPRi... Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500-1 The CRISPR corner By...

...number of factors, including the age of the model organism, injection site, and titer. Addgene’s Viral Vector...production. Neuromethods, 195, 77–101. https://doi.org/10.1007/978-1-0716-2918-5_5 Haery, L., Deverman.... Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389/fnana.2019.00093 McDade,...Molecular Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8...

...Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053 Barlow-Anacker, A., Bochkov...cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371/journal.pone.0180664 Boix, F., Llorente... Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala, S., Aresta-Dasilva...Implant Materials. PloS One, 10, e0137550. https://doi.org/10.1371/journal.pone.0137550 More resources on ...

...Nonetheless, the initial release caused a storm as the organization left open the question of how much data was ...to make the underlying data easily available, organized in a form that can be understood by others, balanced...each individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent...researchers. Yet accurate and comprehensible organization of data is essential for efficient science. ...

...your host organism. Because transcription machinery differs between cell types or organisms, promoters...GFP Resources on Addgene.org Find Plasmids for Your Research     ...  Kendall Morgan...

...Guest blogger Todd Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated...recombination pathway. The Waldman lab at the Georgetown University School of Medicine has used this gene... at the Lombardi Comprehensive Cancer Center, Georgetown School of Medicine. His research currently focuses...Made in Heaven: CRISPR and AAV Resources on Addgene.org Check out our Viral Service Find AAV Vectors...

...rights (2). Less formally, governments are the organizations that make and enforce the rules of the lands...climate change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines... while their website lists over 200 partner organizations, and 514 satellite marches are scheduled to ...Chronicle of Higher Education: “March for Science” Organizer Says It’s About the Public, Not the Scientists...

...Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt...Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri...editing. (2019) Bioinformatics 36:2001–2008. https://doi.org/10.1093/bioinformatics/btz867 Haeussler M, Schönig...selection tool CRISPOR. (2016) Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources...

...Neuroscience Methods, 172(2), 250–254. https://doi.org/10.1016/j.jneumeth.2008.05.003 Ghosh, R., Gilda... Review of Proteomics, 11(5), 549–560. https://doi.org/10.1586/14789450.2014.939635 Gilda, J. E., & Gomes...Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j.ab.2013.05.027 Additional Resources on... and Optimizing a Western Blot Resources on Addgene.org Addgene's Western Blot Protocol  ...

...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...protein are available (Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent Protein Collection Find...

...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...

...in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it! ...including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem...mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University has developed a ...viability. In their recent work in Nature Methods, Georgieva et al describe the rational production of a new...have fewer effects when fused to your POI. Georgieva et al developed the inntags via a bioinformatics...information on inntags, read our recent blog post. Georgieva, et al. Nature Methods. 2015 Aug 31. CRISPR-Display...monitoring cargo trafficking, and for discovering organelle contact sites. This suite of deposited fluorescent...