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We narrowed to 839 results for: LEA

Showing: 281 - 320 of 839 results

  1. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...continuity - two lead organizers on each program, one junior and one senior.  To learn more about the STIN's...generation of science communicators. In this chapter we learn about some of their events - science seminars, science...achieving success. Chapter 9: Science by the Pint - We learn why a "Science by the Pint" event is the best way...about starting your own SciComm organization and to learn about some of SITN's other initiatives, email sitnboston...

  2. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...productive relationships. I will be sharing what I’ve learned along the way in this ongoing series of blog posts...Harvard Medical School researcher Connie Cepko to learn about her mentoring style. Top 7 questions scientists...well as) their own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you ...reach your goals Listens well and communicates clearly and openly Gives honest feedback without ever being... of, and to respect my students as developing colleagues even as I reinforce the necessary power dynamic...

  3. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...Northeastern University, you are expected to spend at least one semester (the majority spend two to three) working...much less specific overview. It was immediately clear that Addgene wanted to ensure applicants understood...in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in the process...spent a lot of time shadowing members of my team to learn what I was to do for my time at Addgene. This training...not been the case for me at Addgene. There is a clear effort to make sure people feel connected.    As...

  4. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ... two labs through the BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate...the molecular mechanisms behind the disease are unclear. Dr. Di Schiavi plans on using C. elegans as a ...treatments for peritoneal mesothelioma. Dr. Nabavi and colleagues have compiled data from primary peritoneal mesothelioma...validate these candidate genes and allow Nabavi and colleagues to prioritize which genes they will use to develop...deserve a voice. Check back with Addgene often to learn of future rare disease research funding opportunities...

  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...specifically in the position. We frequently receive clearly generic cover letters that expound on bench skills...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench research...relatives to network your way to a hiring manager. Learn to confidently describe your strengths in your resume...

  6. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...to maximize productivity and success? We asked leading professors for their top 3 tips. Tom Ellis 1....fluke.” 3. Engage in various projects and help colleagues with their work. “Your one project may be awesome... Ellis recommends that a student interested in leaving academia should present their research at conferences...students to have: working to deadlines, writing up clear and attractive reports, being good at presentations...connected to scientists and use the skills they learned during their studies. They suggest that you make...

  7. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...Deubner et al., 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural...). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could activate...aversive stimuli. In a recent paper, de Jong and colleagues used rAAV FLEx-based vectors to answer this question...containing FLEx technology. Read the genOway press release for more information. References Abdallah, Khaled...hippocampal interneurons that express corticotropin‐releasing hormone." Hippocampus 26.1 (2016): 41-53. PubMed...PMCID: PMC4166436. Resources on the Addgene blog Learn more about FLEx vectors Download our Plasmids 101...

  8. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...complex minus its nuclease component, Cas3, while Type V-K CAST systems use a nuclease-deficient Cas12k..., Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas systems to help them spread...Gelsinger et al., 2024). In either type of CAST, the nuclease-deficient CRISPR-Cas system identifies and binds...barely effective at editing eukaryotic cells — at least, until they met the directed evolution expertise...

  9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ... Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling...HDVR (2X Hepatitis Delta Virus Ribozyme), a self-cleaving ribozyme. In the second-generation SPARC2 system...HDVR is placed upstream of the second attP site to cleave and inactivate any read-through transcripts of ... efficiency of recombination and preferentially leads to gene constructs where the stop cassette remains...

  10. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... vs. SpCas9, see the table below and read on to learn more about GeoCas9.   SpCas9 GeoCas9 Size 1368...ranging from 25-80 °C. Activity is expressed as Kcleave (min-1). SpCas9 has maximum activity from 35-45...experiments tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at ...either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on a gel. SpCas9 is stable when... 30% human plasma. The discovery of GeoCas9 clears the way for CRISPR/Cas9 genome editing in facultative...

  11. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue to...being with the company for about six months, I learned there was a Genzyme-based running team called Running...Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the team ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease ...

  12. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the...reagents so freely with the academic community has clearly made a huge impact on this field. Advances in ...described a chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target... service questions for nearly 7 years, and my colleagues and I have an internal motto for scientists who..., and I found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book...

  13. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    Published
    April 12, 2018, 1:02 p.m.
    ...Foundation, ABRC’s outreach program kicked off with the release of 20 education kits designed for use in K-12 and...ABRC database and ordering platform soon to be released. For individuals interested in ordering from ABRC...has many benefits. Engaging students in active learning through hands-on experiments allows them to develop... out opportunities to engage people in science learning. Whether visiting classrooms, hosting field trips....     Additional Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic Biology into ...

  14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies... relatively low efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of...proteins that lacked TEV. Although we were unable to cleave TEV within the protein we tested, it may be possible...plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys” as the bacteria significantly enhance...

  15. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...Figure 3: When a bison wanders into the campsite, leave your samples behind! Paul and Anastasia (Potanina...hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR ...

  16. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology...Clavularia sp. protein CFP484. Hui-wang Ai and colleagues from the University of Alberta and University... in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used...would like to share with the scientific community, please deposit the plasmids at Addgene! References Ai... to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection...

  17. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral...incorporate into their own labs. This week we are pleased to announce the submission of the results of our... cells (Vandenberghe, 2010). Rather than being released into the cell culture supernatant, the viral particles..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...

  18. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...increasing the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described... rapid quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk...show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying...

  19. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...excitation and emission spectra. Both spectra possess clear maxima where excitation and emission are the most... 500 nm and a global maximum at around 560 nm, leading to different levels of brightness, depending on...check out our blog post on color compensation to learn more about them. Alright, let's build your first...cell culture, the transduction process itself also leads to some cell death. As a result, there is a high...   Conclusion You made it! In this post, you learned about the principles behind fluorophores and their...this process creates visible colors. Next, you learned about how fluorophores can be classified into different...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...attempted to study cancer from every possible angle to leave no stone unturned, but cancer has been producing...between tumors and the immune system We’ll begin by learning how the immune system naturally interacts with...Through interactions with the immune system, tumors “learn” how to escape it. In this process, cancer cells...tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment help create tolerance...enter the clinic, physicians and researchers are learning much about how treatment regimens can be improved...PMC3433802. Additional Resources on the Addgene Blog Learn About Our Cancer Collection Pages Plasmid Grant ...

  21. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...with a microbe, to trigger immune cascades that clear these infections. Anything that generates an antibody...immune system is referred to as an antigen. You can learn more about antibodies in our Antibodies 101 animation...isolate cells expressing this protein. Read on to learn more about antibodies and how to use them in the...produce is not necessarily what you are getting. Learn more about monoclonal antibodies here. Recombinant...simultaneously as well as compare between samples. Learn more about flow cytometry. Cell and tissue labeling...antibodies, the Developmental Studies Hybridoma Bank Learn more about nanobodies Watch our Antibodies 101 animation...

  22. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular...design gRNAs for all the purposes discussed above. Please see videos and text on the website for more detailed...have been “green listed” by several algorithms. Please read the “Detailed Information” text linked to ...and libraries using strategies related to other nucleases as well as inhibitory and activating approaches...PMC5292764. Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips...for a 1st CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries...

  23. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...ride is a great activity for both exercise and clearing your mind. And because the outdoors is so huge...events. Download Addgene’s Science Career guide to learn more about career paths and things you can do to...Plan out meals and snacks ahead of time.  Find learning activities for kids. For example, Skype a Scientist...collected into figures for papers or presentations. Learn a new skill for analyzing and visualizing your data...introductions and literature reviews could make the months leading up to your defense less stressful. Think about...

  24. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...updates to iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here...give researchers the ability to monitor glutamate release from neurons and other brain cells in vivo. Glutamate...protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from the...differentiating between synaptic vs. extrasynaptic glutamate release. Building on the previous generation of the SF-...Resources Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors A Practical Approach...

  25. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...than 75% photobleaching after just 1 minute of exposure. MG-2I-dL5** experienced minor bleaching after longer...ablate cells in culture and in vivo.  Read on to learn more about this killer illumination technique! ...real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization and expressed from ...Sign Up to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on ...

  26. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...guide the joining reaction. BioBrick Assembly will leave scar regions at the site of fragment joining, but...still creating scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our Plasmids ...      Additional Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other...

  27. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear localization ...cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the segments of mRNA to which...occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated...Addgene. Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background...

  28. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...complex processes can’t be overstated, we can also learn a lot from Nature’s simpler solutions to transmitting..., and they can rupture E. coli spheroplasts to release encapsulated proteins. Video by ACS. For a more...appealing target for bioengineering, but it wasn’t clear whether they could 1) be decorated with proteins...Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi...

  29. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...libraries Learn about other members of the Addgene Advisory Board Additional Resources Learn a little...post-doc, Doench worked on using RNAi screens to learn more about cancer biology in Ed Harlow’s group at...and “with CRISPR, just the results are so much cleaner. The time frame from doing your primary screen ...Institute here Read other blog posts about CRISPR Learn more about the Broad Institute and the Platform ...

  30. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...rapid multi-part DNA assembly method. Read on to learn how to use it in your research! The starting point...The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity can be thwarted...available to polymerize, allowing the slower exonuclease activity to proceed. By including only one type...researcher can decide exactly how far the T4 exonuclease proceeds: at the first occurrence of the chosen...overhangs must not contain the dNTP present in the exonuclease reaction or polymerization will occur at that...

  31. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...the following information for a Cas enzyme: Nuclease properties (CasID): Cis/trans activity, PAM requirement... (one of the most frequently used Cas enzymes) cleaves both strands of its dsDNA target in cis and has...generates staggered DNA nicks at its target in cis and cleaves ssDNA in trans. In sum, CasPEDIA documents the ...along with its action on the substrate (nick, bind, cleave, mixed). This information represents the ‘big picture...Perhaps you are a structure junky who just wants to learn about Cas domains. CasPEDIA either directly has ...

  32. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...a team that created a novel form of AI that can learn new tasks and beat humans at the ancient Chinese... side you’re sure to have many opportunities to learn about and develop technologies that will have impacts...pulled tools from across the biological spectrum to learn more about the brain and neurological disease. I...better understand how aberrations in brain biology lead to altered function or maybe your favorite FRET-...pathways. Many scientists came to the Addgene booth to learn more about these tools and to tell us how useful...

  33. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...illumination. This ability to reversibly bind and release a protein of interest in response to light allows...reaches the nucleus where gene expression changes lead to cell proliferation and differentiation. The lab...739201.  Additional resources on the Addgene blog Learn about using nanobodies to control protein activity...Read about RANbodies, reporter nanobody fusions Learn more about using the secondary nanobody toolbox ...Addgene.org Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics...

  34. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...Addgene’s blog to learn about making your own competent cells and our protocols page to learn about bacterial...have studied how HGT occurs in nature and have learned how to introduce genetic materials into cells in... and can include plasmid DNA or fragmented DNA released into the environment. Various factors promote ...more viral particles. Eventually the cell lyses releasing the newly formed viral particles that can infect...

  35. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...inspired you to perform this analysis. Having a clearly stated, specific goal for each analysis will help... currently working in) every time you use them, leave them in their own folder. Doing so will ensure that...same directory where it was run. This technique leaves a trail that further helps you keep track of your... entered is useful for beginners who are still learning the basics. However, these notes take up a lot...Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time and Track...

  36. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ... a date or two for beer, coffee or meals with colleagues you know will attend the conference. Invite others...sure your name tag is always visible. Some people learn names by seeing them. It is OK to ask someone to...the same talk. Read more on this advice to “never leave an empty seat” here. If there are communal meals...especially if they are not in academia. Make sure to learn how to use LinkedIn properly. Then, follow LinkedIn... lunch twice turns a stranger into a friendly colleague. Recommended Reading & More: Scientist Networking...

  37. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...will never ever stop planning, perfecting, and learning, but there are a lot of things we actually don...much potential for service and contribution! Leadership On an organizational level, we keep passing...service and support. The Addgene management team leads with excellence. They are one of the main reasons...the Addgene blog Read our viral vector blog posts Learn more about Addgene Resources on the Addgene website...website Find plasmids for your research  Learn about our viral service Check out job openings at Addgene...

  38. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab...a report to communicate your findings to your colleagues. So perhaps a better definition of “problem solving...challenges we encounter. As you spend time in a lab learning techniques and taking on independent projects,...opportunities to practice problem solving skills and learn to apply them flexibly in a range of contexts. As... organization Time management Managing a team Leadership Resources on Addgene.org Check out our careers...

  39. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...conclusion of inter-homologue recombination was released. This blog has not been updated in response to...to understand what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward...correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic cardiomyopathy. An adult male with... appealing for heterozygous mutations, it will clearly be much more challenging for homozygous mutations...editing rates vary widely from gRNA to gRNA, it’s unclear where this study would fall in terms of editing...

  40. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...possibly be made up of. The answer is… drum roll please… non-coding RNAs! You probably didn’t see that ...activation (CRISPRa) are powerful tools. CRISPRi uses a nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily...targeting ncRNAs. For example, the Cas12a variant can cleave single-stranded RNA using gRNAs to specifically...Charpentier E. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012...induced by CRISPR/Cas9 in the 5’ region of microRNA lead to its depletion and Drosha processing retardance...
Showing: 281 - 320 of 839 results