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We narrowed to 747 results for: Nes

Showing: 281 - 320 of 747 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...Directed Mutagenesis by PCR Google Forums Round Up: First Impressions of NgAgo CRISPR Between the Genes: How...optimize and troubleshoot the system. Site Directed Mutagenesis by PCR & Single Base Editing with CRISPR For ...technology may make some uses of Site Directed Mutagenesis by PCR obsolete! Honorable mentions Of course...

  2. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela DePace...lab culture. The aim of these meetings is to give honest, constructive feedback, set tangible research and...personal development through thoughtful planning and honest feedback DePace’s yearly planning meetings revolve...person organize his or her thoughts to facilitate honest conversation. Goals and Planning Worksheet: ...

  3. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ... more often than other disciplines. The majority of tweets for most disciplines contain scientific domains...#8. Figure 1: Highly Represented Scientific Disciplines on Twitter. Twitter users were identified as ...Follower networks of subgroups of scientific disciplines: social (left), life (center), and physical (...

  4. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce irreversible...another important step forward by dissecting that S. pyogenes protein into three parts. Their efforts yielded... from the “S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together... chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this purpose?...

  5. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...into the following categories: Over 300 empty backbones with 100+ distinct fluorophores to choose from...constructs for biosensing. Over 300 plasmid backbones spanning a rainbow of fluorescent proteins “It...It’s a great collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working...collection with such variety.” In most cases, backbones are available to attach the gene of interest to...

  6. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... Encodes insert(s) A plasmid with one or more genes, protein coding sequences, or other elements inserted...designed for cloning a gene/insert into it. Empty backbones often contain epitope tags or fusion proteins ...Insert *Note: This page will not appear for empty backbones Please enter the name of any gene contained within...Addgene ID in the "Backbone Manufacturer" box for backbones requested through Addgene. For example, you could...Addgene qualifies as BL1 or BL2 as defined by NIH guidelines. I hereby certify that I have disclosed all known...

  7. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...all know that the Fc domain provides structure, determines isotypes, and provides a place for secondary ...cannot survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...induced “stabbing” and “poisoning,” but let’s be honest, the elimination style the immune system is most... that!    References and resources References Clynes RA, Towers TL, Presta LG, Ravetch JV. Inhibitory...

  8. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...currently houses over 890 CRISPR screens in 629 cell lines, each manually curated and annotated from more than...and power of CRISPR-based library screens in cell lines from humans and other species. By accurately compiling...browse through screens performed in relevant cell lines or conditions. This increased ease of access not...screens performed in 26 different breast cancer cell lines. Screenshot updated 2024. Each screen curated...

  9. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type AAV, these two genes are...which contains the AAV structural and packaging genes, the adenoviral helper plasmid which contains the...Next let’s take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products...

  10. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...Giving honest and helpful feedback gets easier with practice so you must build it into your routines. This...their work.  Check out Joanne's Reddit AMA   Guidelines for giving feedback Use formal and informal ...differently? Beware of indirect language You must be honest about performance problems. While giving corrective...

  11. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...raw plasmid sequence and display frequently used genes and control elements. Development of this tool required...feature sequences due to genetic drift and the use of genes from different microbial strains. It proved to be...sequence feature that may be used to make fusion genes, detection needs to occur even if one or two codons... our database, or if you see an opportunity to harness common feature detection in a new way, please contact...

  12. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Kamens several years ago through the Healthcare Businesswomen’s Association and it was through this connection... systematically test the function of all 20,000 genes in disease models and then focus drug discovery ...early age, I was fascinated by the impact that medicines can have on human health. As a teenager in high...the field of gene therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate...

  13. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...capable of robust expression of high G+C content genes, as validated through monitoring the expression ...adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...pooled library from Junjie Chen's lab targets human genes known or suspected to be involved in DNA damage ...

  14. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture ...production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et al., ...hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after cryopreservation (...Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, ...

  15. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ... Addgene to gene-edit mutations into XP genes and other genes involved in photosensitivity in melanocytes...hemiplegia) that are often painful and accompanied by migraines. Those attacks can be brought on by a number of...plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying different SOX10 mutations...

  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... cell type of interest better than immortalized lines derived from the same cell type; however, their ...interest into primary cells, so most primary cell lines require viral infection. A new paper from Niels ...alter protein function or localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis...biallelic mutations. Importantly, hESCs retained their stemness, indicating that iTOP may be valuable for CRISPR...

  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... delete/overexpress endogenous genes or to introduce heterologous genes, but the trend of research has...mimic natural transcriptional programs on multiple genes. Modifying the CRISPRi system for multidirectional...programs to differentially alter the levels of five genes that encode biosynthetic enzymes for violacein, ...

  18. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...screens, enabling genome-wide interrogation of the genes required for cellular processes. For a successful...that constitutively express Cas9 and screen for genes that affect Th2 differentiation and activation, ...-LPs and accelerate the discovery of additional genes and small-molecule probes to aid in the understanding...to different lipid compositions of C. elegans membranes, which might beneficially influence the rhodopsin...

  19. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...keeping the system to minimal size. To do so, they harnessed the tRNA processing mechanism, which is conserved...lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular...for correcting mutations or installing functional genes to study and potentially treat polygenic diseases... that would perturb the unprecedented number of genes with a single viral vector.   Fig. 1: Schematic...

  20. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...medical conditions had been swapped with made up illnesses. They were then asked to evaluate the quality ...methodology makes it easier to spot even minor weakness or limitations in a piece of research (Drummond... mask designs could work to slow the spread of illness. I later helped run workshops where I showed people... jargon that even scientists in neighbouring disciplines might struggle to understand. Why should we trust...

  21. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project progress...identify constraints and boundaries. Where are the lines of authority, responsibility and accountability?...try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation tips...

  22. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...learn new tasks and beat humans at the ancient Chinese game Go). In addition, there was an entire story-telling...neuroscientists are using techniques from across different disciplines to better understand neurobiology and human cognition... provide a highly versatile means of delivering genes to many different cell types with minimal immunological...effects. AAVs are used in experiments targeting genes to specific cell types in the brain, mapping neuronal...

  23. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...cloning uses short sticky ends to insert genes into plasmid backbones, base pairing between these ~4 base ... different types of PCR-generated inserts. By harnessing the power of DNA repair, you can assemble multiple...2024.  References and Resources Further reading Harnessing homologous recombination in vitro to generate...

  24. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity...variable actually makes a big difference for the robustness of the findings.   How do we make research more...code, and other materials. The use of reporting guidelines can also help remind us to include all of the...

  25. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...PAM sequence that the Cas nuclease recognizes determines site accessibility. Cas9s with a broader PAM ...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...

  26. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...observe a phenotype, the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly... al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms tRNA-gRNA and tRNA-gRNA-tRNA...constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs...knockout, or multiple gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also...

  27. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Chen et al. successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the...of fluorescent puncta observed. By labeling two genes of a given chromosome simultaneously, they could...also monitor the spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...

  28. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...clinicians, researchers, and even farmers are harnessing these molecules for good. RNA:RNA dsRNA and ...exploited for antiviral purposes similar to that of vaccines. If you know the sequence of a virus, you could...dsRNA. Synthetic dsRNA Researchers have been harnessing the power of dsRNA for a long time, even before...complete. However, transcription of thousands of genes is constantly occurring throughout a single cell...

  29. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., 2017). However, Acr inhibition...What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires...compatible approach to simultaneously clone two genes-of-interest (GOI) into two independent expression...

  30. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...definitely not worried. I have confidence that from Business Development to Shipping we will execute any new...thanks to evolving outreach tactics, increasing business development efforts, and our London team. We show...Atlantic. We’ve been in documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. ...dedication, flexibility, innovative thinking, and openness to team work.   Company culture Once in awhile...

  31. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion-pump encoding genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction...fluorescent protein. This GEVI features increased brightness and improved plasma membrane specificity. Stable...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...

  32. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...Science Foundation, and Stanford University—that harnesses the creative power of sex and gender analysis ...sex of their cells. For one thing, cells and cell lines degenerate over time—and sex can be hard to determine...indicating the sex of the most commonly used cell lines appearing in American Journal of Physiology-Cell...Engineering, and Environment—a project aimed at harnessing the creative power of sex and gender analysis...

  33. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...directions of the project, Outlines a detailed scope of specific aims, milestones, possible setbacks, and...will address these limitations and lead to new milestones in the field. Research strategy The Research...proposals consist of 3 to 5 aims, with secondary milestones to meet along the way to achieving each aim. ...

  34. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...in your career path and still find comfort and happiness there. Read all of the Meet an Addgenies features...Fabienne Furt, Addgene’s Associate Director of Business Development, got her PhD in Plant Biochemistry...she joined an investment club and discovered the business side of science, she stuck with it. Lab Manager...people, and making them get outside of their comfort zones. I came to Addgene with a goal to learn nonprofit...

  35. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...spike protein can be used for the development of vaccines and diagnostics (Hsieh et al., 2020). As the virus...diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase and Taq..., from Addgene to better understand which human genes are important for SARS-CoV-2 entry into human lung... information on these plasmids and a behind-the-scenes glimpse of their work, head over to their blog ...

  36. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...that takes advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified...long-term solution to reproducibility and cost-effectiveness for broad and large quantity uses. We therefore...accuracy while screening. We also compare different clones of the same target to see if the results are the...primary antibody compared to negative control also determines the signal’s strength. Exposure time is the amount...

  37. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...cells, one may have the unfortunate experience of witnessing…nothing. For some unclear reason, cells are not...electrostatic forces between these two negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic...conditions for storage, transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603...receptor-independent virus adsorption on target cell membranes." Biophysical chemistry 97.2 (2002): 159-172. ...

  38. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... such as LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself. Many researchers prefer...that target the common features of viral vector backbones; once validated these universal primers can be...popular approach utilizes the antibiotic resistance genes carried on transfer plasmids. Target cells are transduced... for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1. PubMed PMID: 15291957...

  39. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown... al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue),...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...

  40. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...H840. To ensure that the clones were capable of cleavage, they tested the clones against an NNN PAM library...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...variants recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition...
Showing: 281 - 320 of 747 results