...understanding the brain’s function is knowing its architecture, in particular the connections between different...to their advantageous characteristics for brain research and their ability to introduce genetic material...fluorescent proteins (red, green, blue). If a researcher injects a cocktail of these different AAV vectors...Atlas website. A similar approach was used by researchers from the Janelia Campus for their MouseLight ...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2...

... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... variety of ways to manage my time. As a busy researcher, you’ve probably already started coming up with...hope you’ll find this post useful. After a bit of research and thinking about my own processes, I’ve broken...

...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite Cas enzyme...generally low; to increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate...repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand...

...variety of organisms.  While much of current CRISPR research focuses on its potential applications for human...engineering tools that are available to academic researchers through Addgene. CRISPR components for plant ...promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos with minimal cloning... hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing...

...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if your...this file over the years and a talk about your research or work will write itself when the time comes....ask about how many people use plasmids in their research and then see how many of them have requested from...

...blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to ...combination with retinal) variations designed to give researchers flexibility in using optogenetic tools in their...concentration gradient, and of activation inhibitory (ArCH) proton pump passing ions against the concentration...proteins activate their signaling pathways allowing researchers greater control over when and where to activate...

...increasing affordability and accessibility, more researchers have been utilizing full plasmid sequencing services...sequencing that Addgene uses.) However, when some researchers received the sequencing results for their plasmids...numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid features that ...monomeric plasmid compared to DH5α. However, more research is needed to determine which recA- strains are...

...135. https://doi.org/10.12688/f1000research.21018.1 Munoz-Lopez M, Garcia-Perez J (2010) DNA Transposons...different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping...encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to cells or a ...

... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic...

...effectiveness and interpretation of RNAi. Fortunately, researchers studying plants and other organisms lacking this...concatenated. hpRNA has been extensively used in plant research and many different constructs are available to...and is a popular RNAi trigger in the mammalian research community. Fortunately, many tools for shRNA in...fluorescence readout of RNAi activity. As a plant researcher, the traditional hpRNA and visual screening method...

...how and where you pursue it. It can manifest via research and data gathering, regulatory efforts, policy...fellowships have a strong focus on early career researchers who have decided to transition out of academia... host “Hill Days” where they train and bring researchers to Congress to advocate for increased science...incentivizing the real-world impacts of health services research. She is particularly interested in driving scientific...

...efficiency. Together, these factors prevent stem cell researchers from generating clonal knock-in lines, especially... allele. For N-terminal tagging, specifically searching for error-free knock-in at both alleles may be...for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing CRISPR/Cas9 genome-editing...Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S...

...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038/s41592-021-01338-6 Grimm...

... an important animal model for research, particularly genetics research, for decades. Its short life cycle...filmed at Addgene:     If you're working in fly research, I hope you find this new technology as helpful...

...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies...vector jokes and comics. Other new resources for researchers using viral vectors In addition to “going viral...

...technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the ...infect many different types of cells. This means researchers who use VSV-G can deliver their genes of interest...autophagy, CRISPR, and so much more (find the full, searchable list here). Tips for using pMD2.G At Addgene,...

...kept Addgene at the forefront of life sciences research for the past decade. We’ve also learned along ...Summaries of the latest technologies and breakthrough research. Insights about how to navigate through all ...graduate student, post-doctoral fellow, lab manager, research technician, or Principal Investigator. How-to...

...publication where you described them. Search for your article as you would search for it in PubMed and choose the... share plasmids. To achieve this goal, we will archive any plasmids you've deposited with us and distribute... your plasmids are online and available to the research community. This article was originally written...

...plasmids? Do you spend a lot of time in PubMed searching for research articles? Well then, my scientist friend...Filters” page, select the category “LinkOut” and search for “Addgene”. Then check the “Link Icon” box next...

...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...we've made to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA...

...Human Development (NICHD) in 1986, to enable basic research by banking and distributing monoclonal antibodies... We have also created the Monoclonal Antibody Research Institute dedicated to: 1) developing new ways...Data Freedom: The Expansion of Data Sharing in Research Publications ...

... Mentor Learn How the Future of Research Looks to Improve the Research Process  Browse our Career Posts... Browse Wu Lab Plasmids Find Plasmids for Your Research  ...

...also noted, papers detailing Nobel Prize-winning research typically weren’t among the most cited. And, as...media too. Services like Mendeley are allowing researchers to track and share their own libraries and offering....0006022 Aragon AM. "A measure for the impact of research" (2013) Scientific Reports 3, Article number: ...

...scientists find the plasmids they need for their research, show labs how they can benefit from depositing...has been exciting to learn about all the amazing research being done with plasmids. I heard an impressive...and Harvard and the McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop ...

...approach, originally published in Nature, is a “search-and-replace” genome editing technique that mediates...efficiency Building upon prior reverse transcriptase research, the Liu lab created and evaluated 19 PE1 variants...Liu lab contributed an improved prime editing “architecture” that can be combined with any of the PE2-PE5...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

...can help fight stress and depression, and now researchers have reported a mechanism based on studies of...exercise, show resistance to depression. For the researchers led by Dr. Jorge Ruas of the Karolinska Institutet...really wasn’t what they’d expected. "Our initial research hypothesis was that trained muscle would produce...

...know we've discussed it before, but CRISPRs allow research to move so fast." On the very same day the monkey... more precise and useful tool. A week later, researchers at the Broad Institute and MIT published another...these editing tools that are more specific to our research needs. We may even be able to alter the type of...

...which gives people more time to focus on their research.  How will your prior work experiences help you...of scientists, the needs of employees, and the research environment.   What do you hope Addgene will accomplish...multidisciplinary group of scientists together to draft a research proposal, which Art in Giving was able to fund...

... in the scientific community to speed COVID-19 research. With the COVID-19 pandemic, we saw the need to...extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular Biology Tools ...design. Best wishes for the new year and your research. See you in 2021! ...

...And on social media, you could be sharing your research or finding opportunities to bring science to the...rest.”- Jason Snair, Product Designer On the job search “Always go for the job you want, even if you don...opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an imposing endeavor.  Break...

...content about timely scientific topics, as well as archived seminar presentations. One of SITN's newest initiatives...hear more about what life is like as a student researcher. The future of DayCon DayCon and other similar...information about SITN: DayCon event website (includes archived content) Science in the News website Check our...

...350,000 individual plasmids to 5,000 different research institutions. This has given us a unique window...," Andrew Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In...Thank you for inspiring us with your breakthrough research and your commitment to scientific sharing. In ...

...A flexible and modular optogenetics tool The researchers also showed just how flexible their LITE system...conventional optogenetic tools." In total, the researchers applied the LITE system to 28 different genes... uses.” Konermann said she is already aware of research groups making use of the new optogenetic tools...

...public access to the results of federally-funded research. 2)      In June, the White House honored 13 “...enterprise lending their support to open scientific research data around the world. 4)      The Annals of Internal...more than 10,500 microarray studies found that researchers who made their data available to others got a...

...give honest, constructive feedback, set tangible research and career goals, and celebrate accomplishments...and Planning Worksheet: Lists accomplishments, research/professional goals and feedback. Calendar: ...principles behind this technique are beneficial for researchers at all stages. Rather than succumbing to academic...

...genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process...genome tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't...Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed to the writing of this article...

...expansion will give researchers increased flexibility when planning their research and expanded choice...and ordering. This split allows us to provide researchers with increased choice and flexibility; while ...

... and disease. To observe bacteria in action, researchers have developed in vivo bacterial reporters that... by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently...Want to start using acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene...

...in the educational sphere, it can range from researching an area, to writing an introduction that gives...mitosis and meiosis in biology. Lots of it is researching the different aspects of the subject you're writing...writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe to the Career...

... portion of lab waste. Despite the fact that researchers represent a minority of the world’s population...environmental impact, but we can all do more to make research more environmentally friendly. Adopt one or all...Subscribe to the Addgene blog Find lab tips for your research Read another blog post about conservation in the...

...families have been characterized. To date, 83% of archaeal and 45% of bacterial genomes utilize this system...translative technologies. Addgene has empowered researchers to harness previous experimental successes and...CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or application that involves...

...DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end joining (NHEJ... efficiency Since the development of BE3, many research groups have made improvements to base editors,...indel frequency. Koblan et al., 2018 BE4max, ArcBe4max, ABEmax   Improved nuclear localization and expression...-0491-6 Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R., Langner, L. M....

...Ford  Listen to the riboglow podcast segment Researchers from the Palmer lab at the University of Colorado...cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast segment The...reporter plasmid! Martin et al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ...

...Arnold  Listen to the MarathonRT podcast segment Researchers from Dr. Anna Pyle’s lab at Yale University recently...transport at the cell surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast...them be analyzed by mass spectrometry allowing a researcher to specifically identify proteins that are enriched...

...Hot Spring at YNP, and since then, many researchers have searched for novel organisms and enzymes from these...our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...

... were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, ...increasing popularity of gene synthesis, many researchers now use codon-optimized versions of common coding... so our detection system was enhanced to allow searches for a perfect protein sequence match even when...

...stories all the time. They provide accounts of their research struggles in papers. They recount accomplishments...expanding the impact and appreciation for their research. Long story short, telling a good story is something.... And you won’t need to dive as deep into your research at an outreach event as you might in a scientific...

...over to work on career skills development. Back in March, we posted these 25 great tips for scientists working... compiled lists of grants: Science: Where to Search for Funding The Ohio State University; Graduate... Database: 100 Places to Find Funding For Your Research University of Florida: Training Grants and Other...

...are fewer outside influences on your topics of research or course of action One can focus on science for...flexibility with the academic calendar Academic researchers must be extreme self-starters and especially ...structures are common Small integrated teams, little hierarchy Real diversity and acceptance can be an issue...

...requirements. To solve this problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo...efficiency. Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...