We narrowed to 331 results for: AGA

1. Save Time and Money by Making Your Own Competent Cells

   Type

   Blog Post

   Published

   Jan. 28, 2014, 3:10 p.m.

   ...plating if your transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’...

2. Science Communication for Everyone

   Type

   Blog Post

   Published

   April 16, 2019, 1:05 p.m.

   ...comments.  Lastly, we are planning to launch an e-magazine this year which would feature articles, posts,...

3. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

   Type

   Blog Post

   Published

   Nov. 29, 2018, 1:53 p.m.

   ...immune systems that provide bacteria protection against infectious agents. Several enzymes behind this ...

4. Why Do I Need an MTA Anyway?

   Type

   Blog Post

   Published

   May 1, 2014, 8:23 p.m.

   ...the plasmid sharing space, we heard over and over again that the legal documents were one of the biggest...

5. Our Most Popular Blog Posts from 2019

   Type

   Blog Post

   Published

   Dec. 31, 2019, 2:26 p.m.

   ... or emailing us at [email protected].  Thank you again for a fantastic year and happy reading. Best wishes...

6. Editor's Choice, September 2016

   Type

   Blog Post

   Published

   Oct. 7, 2016, 4 p.m.

   ...post, it is my pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed...

7. Celebrating Accomplishments in the Lab

   Type

   Blog Post

   Published

   May 8, 2014, 3:07 p.m.

   ...work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and hopefully I'll have worked something...

8. Three Tips to Organize Your Lab Notebooks in the New Year

   Type

   Blog Post

   Published

   Jan. 2, 2014, 3:44 p.m.

   ...access your tasks on the go Never miss a deadline again. Organize you experiments, keep track of upcoming...

9. 2023 and 2024: Reflections and Looking Ahead

   Type

   Blog Post

   Published

   Jan. 23, 2024, 2:15 p.m.

   ...new year, it is a wonderful opportunity to once again reflect on our achievements in the last year and...

10. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type

    Blog Post

    Published

    May 12, 2015, 1:32 p.m.

    ...information about limitations and caveats of Cre usage: Vagaries of conditional gene targeting. Schmidt-Supprian...

11. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type

    Blog Post

    Published

    Aug. 13, 2014, 3:49 p.m.

    ...SpyLigase can be used to link affibodies or antibodies against common tumor markers to subsequently capture cancerous...

12. 9 Tips to Achieve Success in Academia

    Type

    Blog Post

    Published

    April 1, 2014, 5:33 p.m.

    ...the Lab!" and more.   Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for...

13. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type

    Blog Post

    Published

    Feb. 20, 2014, 4:57 p.m.

    ...targets. These pooled libraries include enrichment against kinases, cell cycle proteins, and more.  “In the...

14. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type

    Blog Post

    Published

    Aug. 22, 2019, 12:43 p.m.

    ...you would plate the cells on kanamycin-containing agar. Only cells carrying plasmids that contain a kanamycin...

15. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type

    Blog Post

    Published

    July 16, 2019, 12:59 p.m.

    ...of 5 day old fish. The larvae were immobilized in agar at the head with their tails still free, and different...

16. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type

    Blog Post

    Published

    Feb. 13, 2018, 2:51 p.m.

    ...Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A fused MNase...

17. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type

    Blog Post

    Published

    July 18, 2017, 12:41 p.m.

    ...Cry proteins will then be screened for toxicity against local insect pests. By developing biological agents...

18. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type

    Blog Post

    Published

    June 5, 2017, 3:19 p.m.

    ...undertake and every service we provide is tested against fulfilling our mission. “Addgene’s nonprofit mission...

19. SunTag and Fluorescent Imaging

    Type

    Blog Post

    Published

    March 28, 2017, 2:30 p.m.

    ... inside the cell, in the nucleus and cytoplasm. Again, they found that SunTag marked single molecules ...

20. FastCloning: A Newer, Simpler Cloning Technique

    Type

    Blog Post

    Published

    Nov. 1, 2016, 2:30 p.m.

    ... running a small aliquot of the reaction on an agarose gel. You should see bands with the appropriate ...