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We narrowed to 507 results for: CIT

Showing: 141 - 160 of 507 results

  1. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...on this topic, the authors state, “Both explicit and implicit aspects of today’s postdoctoral training...need to move around every 3-6 years—a short and exciting ride Complicated matrix structures are common...

  2. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...production to individual labs, but now we’re very excited to provide ready-to-use CRISPR lentiviral preps...lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...lentivirus on our blog and protocol pages. We’re excited to provide this new service for researchers wanting...

  3. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...towards conjugates.  Storage   Once you get your exciting new antibody, the second thing you’ll want to ...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ... need to know the wavelength of laser needed to excite them - and it’s a good idea to make sure that laser...

  4. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination...model for this study (Ref 6)). In addition, it was exciting to see that the use of optogenetic tools is expanding...observe the function of immune cells that are not excitable but responsive to calcium. We’d love to expand...

  5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression...

  6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ... delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...

  7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found that YFP and... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...

  8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...rate (Redfern et al., 1999) or altered neuron excitability (Zhu et al., 2014). The system allows you to...important to consider that the same receptor can elicit different responses depending on what cell type... chemogenetic receptors can be used to alter excitability by lowering or raising the threshold for signaling...

  10. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...antibody’s specificity. Strength and accuracy The strength is based on binding specificity and exposure...public service to the science community, and I am excited to be a part of it! If you are interested to get...

  11. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...on the position in the protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions...For applications that require high DNA editing specificity, for example for potential therapeutic approaches...ABE8s demonstrate an overall improved base editing capacity, even at sites that used to be difficult to target...

  13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...trials, wild type AAV is not associated with any pathogenicity and has a low immunogenic profile. AAV was first...new and refined forms of capsids with improved specificity. AAV vectors are able to facilitate and enhance...abilities to infect target tissues. Improved specificity through control of gene expression and the selection...

  14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ... the Science City The conference was located in the modern, relatively new “Science City” area on the...choose to locate there. I made time to go into the city of Guangzhou and see some of the sites. Most fascinating...

  15. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...this, there are close to 10,000 citations for anti-Myc 9E10 in CiteAb, many of which are from this year...Locate a promising monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...proteins for researchers. Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if ...in a rationally engineered Cas9 with improved specificity. Hold this flexible model in your hand for a ...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...history was very much in the making. One of the exciting, and challenging, aspects of talking to 8th graders... proficient in science, regardless of race or ethnicity. Perhaps you don’t have children but are interested...strawberry, or demonstrated that they’re living in an exciting, tumultuous time in scientific history. Whatever...

  19. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds... was moved from one department to another within city government. Throughout these moves and program changes... our program didn’t stay in one place within the city infrastructure. My career path changed when I was...

  20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...programs for K-12 students. You can see why I was excited to be invited to attend. My job is to ensure that...marmorated stink bugs to make them pink. We’re excited to see new tools being developed and shared for...
Showing: 141 - 160 of 507 results