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We narrowed to 427 results for: RAP

Showing: 141 - 160 of 427 results
  1. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...2014.   In vivo uses For both research and therapeutic purposes, antibodies can be used to affect cellular...functional grade antibodies. Some of the research and therapeutic uses overlap: antibodies can bind to and inhibit...investigating signaling pathways or developing therapeutics. Experimentally, antibodies conjugated to fluorescent...
  2. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... before it, offers an interesting view into how rapid innovation affects the research community. I’ve ... not. It is an offline tool, but comes with a graphical user interface. Best strategy for labs: Define...believe the future of CRISPR software is promising. Rapid innovation in this space has put us in a good position...
  3. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...probe into a single molecule often work better for rapid-cycle PCR, such as the LightCycler, as it reduces...chemistry, unless running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr ...brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler, you’ll likely...
  4. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...the dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly...advancing this field and how we are able to support rapid progress in all areas of research. We welcome your...
  5. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...memory, a special type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional...could also be powerful tools for the field of gene therapy if they can reach the affected part of the brain...Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic Injection...
  6. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ... (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid sharing over...and become available into 2021. Check out the infographic below for some Addgene depositor fun facts for...
  7. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...students. As part of this discussion, we mention graphs such as the one shown to the right that illustrate...but many students discuss the third year dip (see graph). Niroshi and Ben give their insights as to what...
  8. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Scientific Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific graphics. PS:...
  9. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...and has huge potential for the development of therapeutics. It also represents a first for Addgene - while...That’s it for now. As I indicated in the opening paragraph, I’m currently on my way back from a very successful...
  10. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...and shared by others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These...Or look at our "10 Years of Plasmid Sharing" Infographic!  ...
  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...
  12. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ... induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies ...phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI:10.1126/sciadv.abl9171...
  13. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used to generate...Cornerstones of CRISPR-Cas in drug development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: ...
  14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...sustained activation period is beneficial for use in therapeutic or behavioral applications where drawn out effects... by Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology...
  15. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...various tools, this may happen over email or using rapid communication tools such as Slack. Share data and...exchange project at the International Cancer Immunotherapy Conference in Frankfurt, Germany When funding...
  16. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...including Activin A. A commercially developed therapeutic antibody that binds Activin A and interferes ...experience is in both vaccine subunit design and therapeutic biologic design (ADCs) along with pre-clinical...
  17. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr.... psychology major and a musician, I found myself rapt by this book! Far from a work of glib pop psychology...
  18. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from... first yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil...
  19. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ... mixture on solid media. The colonies are then scraped off the plates and pelleted for DNA extraction....amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped out, ...
Showing: 141 - 160 of 427 results