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We narrowed to 580 results for: RON

Showing: 141 - 160 of 580 results

  1. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...available with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal...group optimized their initial system by using a stronger promoter and different biochemical secondary energy...AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use...

  2. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...leaves patients incredibly sensitive to sunlight and prone to developing metastatic melanomas and other cancers...,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012, researchers...sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they now think that...that mutations in the gene may prevent neurons from returning to basal conditions normally after periods...

  3. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... chow, a different water filtration system, environmental stability in an incubator), but there’s a lot...for a transparent and collaborative research environment. Journals as enforcers Currently, most of the... formatting can feel like yet another hurdle in front of publication when all they really want to do is... of incentives. Right now, researchers are not strongly rewarded for being transparent in their communication...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See ...Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also ...chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab...with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV...

  5. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...photos of our pets. #SocialDistancing may send a wrong message for some people. We should recommend #PhysicalDistancing...the COVID-19 pandemic, check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health...home, the STEM career exploration site Inventing Heron has a radio reporter challenge, and code.org has...necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is a challenging ...

  6. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...presented chronologically. With a resume however, most often people choose a reversed chronological style ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

  7. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...generation lentiviral packaging plasmid from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet...pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid...generation lentiviral packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used...generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It also...

  8. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...flexibility for in vivo proximity labeling   By Ashley Waldron Proximity-dependent labeling has become a powerful...doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’...contraction, and calcium channels are activated for neuronal action potentials; it’s an ion of interest for...monitor calcium specifically in their favorite neurons. This specificity is feasible partially because...Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct Vol. 13; 1662...

  10. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...beginning. The well-characterized bacterial lac operon contains a gene called lacZ that encodes for the...the enzyme β-galactosidase. Expression of the lac operon is induced by lactose, and also by a lactose analogue...completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac expression). When...in the medium to ensure transcription of the lac operon.   Tips for blue-white screening Use a good control...

  11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...separated by 28 base Csy4 recognition sites from a polycistronic transcript. Although this method shortens construct...requires coexpression of Csy4 with Cas9 and the polycistronic transcript. As nucleases may have off-target... of gRNA cleavage.   Xie et. al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms ...Bacteria and viruses have illustrated the power of polycistronic gene organization, and its translation to CRISPR...

  12. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...difficult to culture outside of their natural environment. Thus, the Harris Wang lab at Columbia University...gut microbiome members in their native habitat (Ronda et al., 2019). They called their method Metagenomic...19465049. PubMed Central PMCID: PMC2752045. Myhrvold, Cameron, et al. "A distributed cell division counter reveals...PMID: 26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the...

  13. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...the appropriate ligand - estradiol for hER or progesterone for hPR - leads to nuclear localization, binding...controlled transcription factor and a ZIF268/progesterone controlled transcription factor as well as reporters...with various concentrations of estradiol and progesterone and showed that reporter expression is dependent...cognate inducer. Since induction via estradiol and progesterone do not affect each other, these systems can ...

  14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...autophagy. Morphological assays Electron microscopy: Electron microscopy is a traditional method for...at various stages of degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged...will fluoresce green unless it’s in an acidic environment like the vacuole or lysosome. See Table 2 for...

  15. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...sex and gender analysis important? Doing research wrong costs lives and money. According to the US General...treated, and other hormonal, immunological, and environmental factors. How to include sex in experimental ...Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing the creative power...Evoloving Check Out Extracurricular Activities for a Strong Science Career Path Additional Resources Check...

  16. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin, both of which...modifying the antibody sequence or that rely on strong protein-protein interactions with other peptides...of non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions with other peptides... Ashley Waldron...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...created a dual-deaminase editor called SPACE (synchronous programmable adenine and cytosine editor) by ...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....

  18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...enzymatic fusions, such as dCas9-TET, can, like the iron ball-and-chain that they resemble, actually impede...unmethylated CpG to behave naturally in the nuclear environment. One good system to balance these needs is the...

  19. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we know and love, the FLAG...

  20. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    .... Pulse generation (TTL pulse) is a standard electronics protocol not specific to lasers or optogenetics... Use of channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial ... Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless optogenetics... et al. A simple head-mountable LED device for chronic stimulation of optogenetic molecules in freely ...
Showing: 141 - 160 of 580 results