...presented chronologically. With a resume however, most often people choose a reversed chronological style ...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

...generation lentiviral packaging plasmid from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet...pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid...generation lentiviral packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used...generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It also...

...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...flexibility for in vivo proximity labeling   By Ashley Waldron Proximity-dependent labeling has become a powerful...doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...

...The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’...contraction, and calcium channels are activated for neuronal action potentials; it’s an ion of interest for...monitor calcium specifically in their favorite neurons. This specificity is feasible partially because...Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct Vol. 13; 1662...

...beginning. The well-characterized bacterial lac operon contains a gene called lacZ that encodes for the...the enzyme β-galactosidase. Expression of the lac operon is induced by lactose, and also by a lactose analogue...completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac expression). When...in the medium to ensure transcription of the lac operon.   Tips for blue-white screening Use a good control...

...separated by 28 base Csy4 recognition sites from a polycistronic transcript. Although this method shortens construct...requires coexpression of Csy4 with Cas9 and the polycistronic transcript. As nucleases may have off-target... of gRNA cleavage.   Xie et. al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms ...Bacteria and viruses have illustrated the power of polycistronic gene organization, and its translation to CRISPR...

...the appropriate ligand - estradiol for hER or progesterone for hPR - leads to nuclear localization, binding...controlled transcription factor and a ZIF268/progesterone controlled transcription factor as well as reporters...with various concentrations of estradiol and progesterone and showed that reporter expression is dependent...cognate inducer. Since induction via estradiol and progesterone do not affect each other, these systems can ...

...autophagy. Morphological assays Electron microscopy: Electron microscopy is a traditional method for...at various stages of degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged...will fluoresce green unless it’s in an acidic environment like the vacuole or lysosome. See Table 2 for...

...difficult to culture outside of their natural environment. Thus, the Harris Wang lab at Columbia University...gut microbiome members in their native habitat (Ronda et al., 2019). They called their method Metagenomic...19465049. PubMed Central PMCID: PMC2752045. Myhrvold, Cameron, et al. "A distributed cell division counter reveals...PMID: 26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the...

...capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin, both of which...modifying the antibody sequence or that rely on strong protein-protein interactions with other peptides...of non-canonical amino acids or peptide tags. 5) Strong protein-protein interactions with other peptides... Ashley Waldron...

...sex and gender analysis important? Doing research wrong costs lives and money. According to the US General...treated, and other hormonal, immunological, and environmental factors. How to include sex in experimental ...Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing the creative power...Evoloving Check Out Extracurricular Activities for a Strong Science Career Path Additional Resources Check...

...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...created a dual-deaminase editor called SPACE (synchronous programmable adenine and cytosine editor) by ...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....

...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags we know and love, the FLAG...

...and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S...enzymatic fusions, such as dCas9-TET, can, like the iron ball-and-chain that they resemble, actually impede...unmethylated CpG to behave naturally in the nuclear environment. One good system to balance these needs is the...

.... Pulse generation (TTL pulse) is a standard electronics protocol not specific to lasers or optogenetics... Use of channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial ... Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless optogenetics... et al. A simple head-mountable LED device for chronic stimulation of optogenetic molecules in freely ...

... relaxed phone call than when first confronted on a stage in front of a crowd. The moderator will then...logistics To make a really lively discussion, I strongly suggest asking the panelists not to use any slides... turnout). This gets people to sit close to the front so the interactions are better. Have a good microphone...

...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...tagging endogenous proteins with an auxin-inducible degron (AID) tag to generate proteins which can be rapidly...Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.” Cell Rep.15... Michelle Cronin...

...species, such as the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. ...CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated. Mobile-CRISPRi...non-model bacteria including pathogenic strains and environmental isolates. For example, Peters and colleagues... remained constant, with fitness effects more pronounced at 12 doublings than at 6. Key takeaways of Mobile-CRISPRi...

... to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV vectors expressing...this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are now available as a...

...many researchers remain unaware that EGFP/GFP is prone to forming noncovalent dimers. This property of ... matter of concentration Most FPs in nature are prone to dimerization (i.e. EGFP) [1, 2] or even form ... fused to the POI. These structures are large (microns), bright, non-physiological, and difficult to ignore...CFP and YFP dimers can form in the absence of environmental changes that the biosensor is designed to detect...