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We narrowed to 749 results for: SOM

Showing: 141 - 160 of 749 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...   Both gradients can be informative, so spend some time thinking about which type of gradient can help...to consider changing your incubation temperature. Some proteins aggregate at 95 °C, a common lysis temperature...Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting for your timer... blocking, first check your antibody spec sheet. Some antibodies work better with specific blockers, and...overnight or room temperature for 1–2 hours, there is some evidence that antibodies may need longer to reach... washes — although this can be harsh and inhibit some detection reactions. Like blocking, you don’t want... to be the most straightforward. While there are some things you’ll want to consider (see our “technical...

  2. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...isogenic AdVs encoding TALENs targeting the same chromosomal region. The CRISPR/Cas9-derived RNA-guided nuclease-induced... will interact with sporadic double-stranded chromosomal DNA breaks “that always happen naturally.” These...Gonçalves says that advantages of AdVs include their episomal nature and very efficient introduction of DNA ...also be used to deliver Cas9 and DNA templates in somatic cells of adult animals in order to create animal...Ventura A (2014) In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature...Weng Z, Gao G, Xue W (2015) Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse ...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    .... Ribosome display The Seeger lab starts with a “pre-enrichment” selection step using ribosome display...which causes the ribosome to pause at the end of the mRNA molecule, forming a ribosome complex consisting... remain folded, and are often less abundant than some other proteins. The Seeger lab developed a robust...consisting of the translated protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies...

  4. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...visitation days can be, at times, very overwhelming. As someone who applied to PhD programs in Biology around the...speaking, the PhD application process tends to go something like this:   However, as simply as it seems laid...complicated process even more difficult. I’ve learned that some of the technologies that helped us navigate a global...program. An email to a potential PI should go something like this: Introduce yourself, brief educational...truly feel about the program and faculty. Here are some questions to consider for your online meetings:  ...prospective students; an online meeting can take some of that pressure off. This question and answer ...

  5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...how they work to overcome them, and why she spends some of her time engaging with others on Twitter, among...switching to Golden Gate is a conceptual barrier. Sometimes people don’t know how to start. They might want...protein-protein interaction work and we’ve gotten some tools back from Addgene recently to explore their...Most Sainsbury scientists are active on Twitter. Sometimes it’s a good way to find out what’s happening in...experiences, articles about women in science, and the sometimes stupid or uncomfortable things that have happened...plant science. I think their chances of doing something amazing will be equally high as for those who ...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...journal club. Some are led by a single individual who walks through the paper figure by figure. Some follow ...calendar. So you want to start a journal club? Here are some things to think about. Choosing a topic and gauging...software repositories, or other information needed by someone to critically discuss the paper.  Before the first...receive include the sense that people learned something new? Did the meeting begin and end on time? Did... their purpose has been served. I hope that with some of the information described above you feel empowered...

  7. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...vector, but retroviruses have been developed into some of the most widely used tools in molecular biology...developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen... as the other components of the vector particle, some of the RT protein gets packaged inside of the vector...viruses and transduction by vectors (which we sometimes still call “infection” as a shorthand, although...double stranded DNA is integrated into the host chromosome, and this is where viral vectors and natural ...express. In the case of a natural virus, this new chromosomal locus is considered “proviral DNA” (Rowe et al...

  8. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...the journey of transformation and storage begins. Some samples are submitted as bacterial colonies in petri...inspected to verify that the samples arrived intact. Some deposits are sent from labs nearby in Boston and...packages have spent days to weeks in transit and some have been transported across oceans and continents...barcoded tube (see next section) and every time something is removed from this tube, the barcode on the ... received is immediately transformed and stored. Some samples need to be transformed with a micropulser...electric charge to help the DNA enter the cells. Some samples require a reduced antibiotic concentration...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...acids. This is termed codon usage bias. In fact, some species are known to avoid certain codons altogether.... tRNAs bind to and deliver amino acids to the ribosome where they are incorporated into the growing polypeptide... Figure 1: An overview of peptide synthesis. A ribosome interacts with mRNA and charged tRNAs to build...critical for determining the rate of translation. The ribosome may slow and pause during elongation which may...synonymous mutations which have even been linked to some diseases. It has been reported that 5-10% of human...suggest codon optimization should not be used in some in vivo experiments and in biotechnology and therapeutics...

  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...celebration, we thought it only fitting to share some stories of ways we’ve turned the available into ... coming from researchers outside the USA, having some materials available to order, yet not consistently... Sinapse Biotecnologia 2022 Brazil   In some cases, the distributors broadly helped increase ...means that our shipping team is always engaged in some form of problem-solving. When universal solutions...and its parts that helps the viewer visualize something that cannot be seen with the naked eye. In 2023...that we could identify the need for distributors in some countries. ‌We’ve developed systems to allow for...

  11. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...application, but it can certainly help you stand out.  In some fields, a cover letter is vital, particularly in...re applying to the specific company or lab. Take some time to research the lab or company via their papers...the beginning is often the hardest part, here are some examples to get you started! Dear Dr./Professor...skills for the position]. I am enthusiastic about [something that you love and is important for the job]. ... okay to start from a standard version, but take some time to make it fit the job you’re applying for....review grammar and spelling several times and ask someone else read your cover letter (as well as your resume...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...concern in healthcare today, but it doesn’t have to be some looming, abstract, convoluted scientific concept...not naturally resistant but has become resistant somehow overtime. One fantastic example of intrinsic resistance... drives microbes to develop survival mechanisms. Some microbes even make their own antibiotic compounds...such a rich diversity of microbes in the world if some didn’t develop, either through random mutation or...Drug resistance is not a black or white issue. Sometimes bacteria are resistant to lower concentrations... will be killed by a higher concentration, or sometimes more exposure time is needed for the drug to work...

  13. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...easy-to-understand set of PowerPoint slides. What are some of the more challenging parts of the job? With fast...goal posts” as a part of a case, consulting can sometimes be challenging. The fast paced nature of projects...of great output in a short amount of time can sometimes become cauldrons on the verge of boiling. On other...sciences, in business, in healthcare economics, or in some combination of these fields. I got my PhD a couple...performing in the market. Hence, as a PhD, an MD, or someone trained in any aspect of research in biology or...successful career in several different fields. While some people continue to enjoy the challenges of healthcare...

  14. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...the next steps on some common applications, join us as we get into the weeds on some of our favorite topics...always inspired by the new seasons, so we've made some big summer plans this year! New Blog Series We ... and tricks for those new to the lab, along with some behind-the-scenes peaks of Addgene’s people, culture..., video, and social media endeavors - in between some much-deserved vacations, sunshine, and beach trips...

  15. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...cold enough to keep some common types of reagents stable for years. In fact, some scientists speculate...sustainable practices in the lab, where I’ll list some easy but effective habits you can incorporate to...motivated and to feel accomplished. In addition to doing some of the quick things above, try joining or starting...your university could join. Find alternatives for some of the harmful chemicals in your lab. These chemicals...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...to look for them? Or maybe you just want to make some really beautiful images? Multiplex IF may be just...similar to standard immunofluorescence, but with some extra considerations (Im et al., 2019; McLaughlin... antibodies from Addgene), or be prepared to run some extra controls and validate the antibody yourself...; this helps all viewers, especially if you have some complex patterns with several overlapping targets...experimental design. Like selecting an antibody, some of the requirements are similar to standard IF. ...being damaged by all the handling steps, or there’s some unexpected cross-reactivity. Keep in mind that you...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...irreproducibility with some solutions based on what I’ve learned. I hope you can apply some of these solutions... “reproducible” does not necessarily mean that someone has replicated it precisely. You can find a great... body works, generating new therapeutics, and sometimes formulating new biological concepts. However, ...ridiculous to expect that one should be able to apply someone else's new and incredibly useful technique simply...experiments. Biology is messy enough that by chance, some small number of the scientists will get a result...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...nucleases are promiscuous. In other words, they can sometimes localize to unintended sites with sequence similarity...In this instance, a 5% off-target frequency is something to keep in mind, but likely will not have a significant...will likely be useful. Optimal gRNA selection With some CRISPR experiments, there is hardly any flexibility...bound by Cas are sequenced, has been tested with some success. The major con is that binding by Cas does...suitable. How to control for off-target events Sometimes there just isn’t a way around off-targets. Your...even when you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled...

  19. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...how they’re used, see some new techniques that take advantage of them, and give some tips on deciding which... organisms and their characteristic radiance for some time – there are records describing them dating ...realized that, although they are not bioluminescent, some types of corals and anemones are vibrantly colored...able to report new fluorophores like DsRed from Discosoma sp. and Katushka/mKate from Entacmaea quadricolor...

  20. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ... in the lab. The first post focused on streaming some lab waste for recycling- a process that’s integrated...well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability, and find...single-use is now a popular theme in life sciences, some of these plastics are high grade and can be washed...markets. Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years...practices to meet higher environmental standards. Find some easy action items on our other article in this series...
Showing: 141 - 160 of 749 results