We narrowed to 367 results for: his

1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...Alabama in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...

2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...start site in those 15,977 human genes. Weissman and his colleagues demonstrated in a paper in Cell that they...

3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses on the enzyme hydropersulfide-propagating... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression...

4. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...Allinen et al., 2004). In fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had...breast tumorigenesis: Friend or foe?." Histology and histopathology (2018): 18021-18021. PubMed PMID: 29978449...

5. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...them easy to harvest from cell culture media. Their His tags allow for further purification, if desired, ...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

6. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...paper to understand what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will ...

7. Addgene: The First Twenty Years

   Type

   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ...Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016, we also began...

8. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...this cloning technique! Many thanks to Rob Hurt for his help on this piece. Rob is postdoc in Peter Schultz...

9. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   Published

   July 23, 2015, 2:30 p.m.

   ...speech recognition, he returned to school to merge his interests in biology and computing. In 2009, he founded...

10. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    Published

    Feb. 11, 2014, 12:13 p.m.

    ...this if he wants data by next week!”, and “This is his job!” filled my naive mind. Moving forward from that...

11. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...-lytic insect cell expression systems (sf9, Sf21, Hi-5 cells)  that allow for continuous expression of...

12. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which...

13. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ...John Doench reports even the greenest members of his lab, high school interns, were able to use Fragmid...

14. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ...I've attempted to answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can...

15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she...

16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...developed and demonstrated by Manuel Gonçalves and his colleagues at Leiden University Medical Center are...

17. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ... poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You also don’t have to...

18. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ... who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard and essential...

19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ... at the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has had ...