We narrowed to 363 results for: his
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TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....this cloning technique! Many thanks to Rob Hurt for his help on this piece. Rob is postdoc in Peter Schultz...
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RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....them easy to harvest from cell culture media. Their His tags allow for further purification, if desired, ...targeted the following antigens: GFP, RFP, the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....Allinen et al., 2004). In fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had...breast tumorigenesis: Friend or foe?." Histology and histopathology (2018): 18021-18021. PubMed PMID: 29978449... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses on the enzyme hydropersulfide-propagating... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....paper to understand what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....speech recognition, he returned to school to merge his interests in biology and computing. In 2009, he founded... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....this if he wants data by next week!”, and “This is his job!” filled my naive mind. Moving forward from that... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....John Doench reports even the greenest members of his lab, high school interns, were able to use Fragmid... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....-lytic insect cell expression systems (sf9, Sf21, Hi-5 cells) that allow for continuous expression of... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....) from the two yeast species into the pYES2.1/V5-His yeast overexpression vector. This plasmid contains...promoter. The cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m....I've attempted to answer several emails that start: “Hi! We ordered something from you and it’s wrong. Can... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m.... can be found climbing mountains or playing with his two cats. Keira Havens grew up in Hawaii where she... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support for, this... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m.... at the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m.... who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard and essential... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m.... poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You also don’t have to... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....developed and demonstrated by Manuel Gonçalves and his colleagues at Leiden University Medical Center are... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has had ... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....Centre for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the...