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We narrowed to 809 results for: org

Showing: 141 - 160 of 809 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

  3. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman N. (2012)...Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/   Uhlen M, ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources on the ...The Basics of Western Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies...

  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...PubMed PMID: 10470036. 3. Said, H. M. “Biotin: The forgotten vitamin.” American Journal of Clinical Nutrition...

  5. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...through your organization in this field. Please also enter the billing address for your organization. We will...customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! Today we’...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...

  6. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

  7. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    Published
    March 17, 2021, 2:40 p.m.
    ... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful!  I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption... Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of...

  8. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...These molecules occur naturally in bioluminescent organisms, but their real power lies in the clever ways ...Although humans have known about bioluminescent organisms and their characteristic radiance for some time...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first ...be responsible and began studying these marine organisms to find the culprits. He even obtained samples...

  9. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...as a free event! Organizing a science conference for the public How does one organize a public scientific...association with the Harvard graduate student organization Science in the News (SITN), we recently sponsored...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...opportunities to improve our communication and organizational skills provide great training for a number ...

  10. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even...knowledgeable staff is here to help. Contact us at mta@addgene.org or +1 617-225-9000, or check out our MTA reference...

  11. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene...on the antibodies. Nature 521:274–276. https://doi.org/10.1038/521274a  Bradbury A, Plückthun A (2015)...antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L...antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787  Taussig MJ, Fonseca... the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995  ...

  12. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...

  13. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596-023-00927-3 Klompe, S. E., Vo, P...integration. Nature, 571(7764), 219–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova...States of America, 114(35), E7358–E7366. https://doi.org/10.1073/pnas.1709035114 Witte, I. P., Lampe, G....transposase. Science, 388(6748), eadt5199. https://doi.org/10.1126/science.adt5199 Additional Resources on...Flexibility to CRISPR Editing Additional resources on Addgene.org Addgene's CRISPR Guide (Jump to Large Edits)...

  14. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying...sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps.....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

  15. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

  16. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...topics in their area of research. The society also organises PhD information events and summer internship information...support each other and provide advice. The society organizes numerous recreational and educational events throughout...for their students. Another large goal of the organisation is to encourage iGEM alumni to mentor younger...Bloggers Learn How to Get Involved in a Profession Organization ...

  17. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific... well. Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus...

  18. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...and impact of your work. Please email deposit@addgene.org if you would like to contribute to the catalog...share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you have suggestions...immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone, ME...Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury A, Plückthun...antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources We would also like to...

  19. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma...Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner R (1980) ...National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank...efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources on ...

  20. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing...labeling Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for... Diessl et al., Microbial Cell, 2020. https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent... tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/s41598-019-53722-0 ...
Showing: 141 - 160 of 809 results