We narrowed to 817 results for: org

... spatial genome organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen, Y., Zhang...biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional Resources... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...

...Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J et al. (2020...SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger...Camelid Antibodies. Cell 181:1004–1015.e15 . https://doi.org/10.1016/j.cell.2020.04.031 Zimmermann I, Egloff...proteins. Nature Protocols 15:1707–1741. https://doi.org/10.1038/s41596-020-0304-x Zimmermann I, Egloff ...trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources on the...101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection Browse ...

...handbooks/Springer protocols (pp. 245–260). https://doi.org/10.1007/978-1-0716-4358-7_20  Karmakar, S., Panda...Biotechnology Journal, 22(10), 2950–2953. https://doi.org/10.1111/pbi.14416  Maher, M. F., Nasti, R. A.,...meristems. Nature Biotechnology, 38(1), 84–89. https://doi.org/10.1038/s41587-019-0337-2  Molla, K. A., Sretenovic...editors. Nature Plants, 7(9), 1166–1187. https://doi.org/10.1038/s41477-021-00991-1  Qiao, J., Zang, Y....virus-based delivery in wheat. Nature Plants. https://doi.org/10.1038/s41477-025-02023-8    Additional Resources... in Popular Culture Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and ...

...Analytical Chemistry, 83(12), 4327–4341. https://doi.org/10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson...Academy of Sciences, 74(12), 5463–5467. https://doi.org/10.1073/pnas.74.12.5463  Shendure, J., & Ji, H....Nature Biotechnology, 26(10), 1135–1145. https://doi.org/10.1038/nbt1486  Slatko, B. E., Gardner, A. F.,...Protocols in Molecular Biology, 122(1). https://doi.org/10.1002/cpmb.59  Wang, Y., Zhao, Y., Bollas, A....Nature Biotechnology, 39(11), 1348–1365. https://doi.org/10.1038/s41587-021-01108-x Additional Resources...Plasmid Quality Control Additional Resources on addgene.org Molecular Biology Reference Sequence Analyzer...

...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic...

...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...Biology. Springer New York, pp 299–311. https://doi.org/10.1007/978-1-4939-8935-5_26 Stuurman N. (2012)...Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/   Uhlen M, ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995 Additional Resources on the ...The Basics of Western Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin...PubMed PMID: 10470036. 3. Said, H. M. “Biotin: The forgotten vitamin.” American Journal of Clinical Nutrition...

...through your organization in this field. Please also enter the billing address for your organization. We will...customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! Today we’...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...

...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful!  I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption... Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of...

...These molecules occur naturally in bioluminescent organisms, but their real power lies in the clever ways ...Although humans have known about bioluminescent organisms and their characteristic radiance for some time...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first ...be responsible and began studying these marine organisms to find the culprits. He even obtained samples...

...as a free event! Organizing a science conference for the public How does one organize a public scientific...association with the Harvard graduate student organization Science in the News (SITN), we recently sponsored...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...opportunities to improve our communication and organizational skills provide great training for a number ...

... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even...knowledgeable staff is here to help. Contact us at mta@addgene.org or +1 617-225-9000, or check out our MTA reference...

...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...

...Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene...on the antibodies. Nature 521:274–276. https://doi.org/10.1038/521274a  Bradbury A, Plückthun A (2015)...antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a  Roncador G, Engel P, Maestre L...antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787  Taussig MJ, Fonseca... the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski ...of antibodies. Nat Methods 13:823–827. https://doi.org/10.1038/nmeth.3995  ...

...the other hand, the tail sequence is much more forgiving and can be mostly or entirely replaced with DNA...Nucleic Acids Research, 48(15), 8601–8616. https://doi.org/10.1093/nar/gkaa605  Whittaker, M. N., Testa, L.... Nature Biomedical Engineering, 1–13. https://doi.org/10.1038/s41551-025-01545-y  Yin, H., Song, C.-Q...Nature Chemical Biology, 14(3), 311–316. https://doi.org/10.1038/nchembio.2559 Additional resources on the...Adenine Base Editors Additional resources on addgene.org Read the CRISPR guide Download the CRISPR 101...

...transposases. Nature Protocols, 19(3), 752–790. https://doi.org/10.1038/s41596-023-00927-3 Klompe, S. E., Vo, P...integration. Nature, 571(7764), 219–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova...States of America, 114(35), E7358–E7366. https://doi.org/10.1073/pnas.1709035114 Witte, I. P., Lampe, G....transposase. Science, 388(6748), eadt5199. https://doi.org/10.1126/science.adt5199 Additional Resources on...Flexibility to CRISPR Editing Additional resources on Addgene.org Addgene's CRISPR Guide (Jump to Large Edits)...

... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...proteome? Open Biol. (2019) 9:180241. DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying...sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps.....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...