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...expression at certain loci to create changes in phenotypes. For example, CRISPR activation has been used...and Gersbach, 2015; Zetsche et al., 2015). These types of CRISPR activation are also reversible: once the...et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post covering...

...There are many, many different types of experiments carried out by scientists every day. Although the...plasmids would address and answer these questions. Types of control plasmids Part of planning your experiment...expressing the independent variable. There are two types of replicate controls: technical and biological....

...require interaction with the data authors. In some types of research, raw data requires huge amounts of storage...calling it a “sisyphean task” given the endless types of data). Rather, PLOS appears to leave the scope...provide data in formats exported to common file types? And how long should data be archived? Simplifying...

...Because transcription machinery differs between cell types or organisms, promoters must be similarly variable...derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters...an enhancer region. Can be silenced in some cell types. EF1a General expression mRNA Strong mammalian...

...bind to epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs...simply need to label the lineage markers of all cell types except CD8+ T cells. We might choose the markers...

...lymphocyte population from a tumor sample. Many cell types can be found in the tumor microenvironment (tumor...Around 34% of the cells are neither of the two cell types (likely B cells and others). Almost no cells stain...in this blog post. First, you learned about the types of scattering that occur when a cell is pulsed by...

...specific 3-dimensional epitopes. Different sample processing steps can alter epitopes in different ways, ...my cells,” or “How does this mutation impact the types of cells found in my tissue.” It is based on the...

...DNA viruses, such as adeno-associated virus, herpesviruses, hepatitis B virus, adenoviruses, and human ...linear proviral DNA is poised for integration, two types of circular episomes can be generated through either...This makes IDLVs an attractive choice for certain types of clinical or basic research applications. Considerations...

...to admit that paper-based record keeping barely copes with the growing amounts of digital data generated...functionalities with experiment records. Many different types of ELNs are available on the market today, ranging...showed that It is important that an ELN supports all types of data, which enables researchers to store everything...

...target site sequence at the site of insertion.  Types of transposons Transposable elements are split into...the size of their host’s genome.   There are two types of Class 1 TEs: those with long terminal repeats... used in the lab While there are many different types of transposons, DNA transposons are most commonly...

... knock-in efficiency across different stem cell types. This has facilitated easy generation of clonal ...choice for epitope tagging in diverse stem cell types. Hundreds of genes can be epitope-tagged in a couple...large protein cohorts in diverse mammalian cell types. A detailed step by step protocol for the csRNP ...

...is induced by various types of interactions with their environment. These types of interactions to a large...growth properties, migration abilities, or other phenotypes that can be efficiently measured at the single...

.... There are numerous variants for the different types of opsins available and their differences and uses...See the Deisseroth lab homepage for details about types of constructs available, instructions for obtaining...cell-type specific opsin expression. However, the types of opsins you are able to use are obviously limited...

...different scoring algorithms and parameters. This tapestry of ideas is what makes choosing the right tool...input in order to generate results. While different types of input are more user-friendly than others, this...less concern than data output as different input types can be readily converted.  Examples: “organism”,...

...and mumps. Sendai transduces a wide range of cell types and replicates in the cytoplasm independent of the...and protein delivery are better suited for these types of studies. What cells are reprogrammed will also...also affect the outcome, since not all cell types are easily attainable, or easy to reprogram. Basically...

...genome for genes that regulate a wide variety of phenotypes, including but not limited to cell growth and...chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts are not...create protein expression constructs fused with epitopes or fluorophores, generate expression vectors with...investigating hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID:...

... proteins coat the virus and help determine the types of cells that it can infect. The VSV-G envelope ...it enables lentiviruses to infect many different types of cells. This means researchers who use VSV-G can...

... be packaged in your choice of five different serotypes.   We are so excited for all the ways this much-requested...Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde (AAVrg...

... joined the Addgene Board of Directors where he hopes to help keep us focused on our core mission to promote...using a decellurized scaffold as a base. Darrell hopes that further advancement of this technique will ...

... she is currently working on a version that she hopes will change the intracellular localization of RNA...Her lab is currently investigating the issue and hopes to have a better understanding of the phenomenon...