...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., ...and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...

...Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032 Chrousos GP, Mentis A-...threatens diversity. Science 367:749.2-750 . https://doi.org/10.1126/science.aba8039 Cordova FA (2016) Embrace...Embrace uncertainty. Science 351:994–994 . https://doi.org/10.1126/science.351.6276.994 Moore E (2021) One...I built a network of mentors. Science. https://doi.org/10.1126/science.caredit.abi6939 Additional resources...

...Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová M, Buriánová...Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong...Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2...for working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols...

...of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry...Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim ...instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources ...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based therapeutics...Ghana Modulate Cancer Signalling Resources on Addgene.org Check out Our Cancer Collection Pages Use the...

...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...how to manage them without them paralyzing an organization. I think these are things that I can bring to...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...

...research with the community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular...Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent...101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick...

...scientists to edit the genome of virtually any organisms on this planet, CRISPR-Cas systems were merely... to quickly detect the presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing...Download our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR...

...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... Kendall Morgan...

.... Even more remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with...antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H...biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...

...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled... Kendall Morgan...

...sports, movies, and just about anything. As our organization grew, we continued this tradition. We now have...delicious) team building activity that is easy to organize. We’ll dedicate a post to these competitions in...Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than emergency ...

...Set up your own Science by the Pint series and organize evens where scientists can grab a beer and chat...institutional knowledge and continuity - two lead organizers on each program, one junior and one senior.  ...For more info about starting your own SciComm organization and to learn about some of SITN's other initiatives...

...lab do for fun as a group? The Ellis Lab self-organizes cinema nights, game nights, and trips to eat out...flexibility and you aren’t locked into a schedule.” George Church comments on how he would go home in the ... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...

...reagent type, target species, target name, host organism, isotype, clone details, vendor, method, tissue...tissues. Nature Protocols, 17(2), Article 2. https://doi.org/10.1038/s41596-021-00644-9 Radtke, A. J., Kandov...Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 More resources on the Addgene...

... happy to have the support of the following organizations to share materials more broadly to accelerate...to industry? Over the past 8 years, several organizations have made their plasmids available beyond academia...to industry, please reach out at techtransfer@addgene.org and we will initiate a conversation with you ...

...collections pages, please shoot us an e-mail at help@addgene.org. We’d love to hear your feedback. Additional ...set of plasmids you’ve deposited with us. Don't forget to get yourself out there! Additional Resources...Inside Addgene Posts Additional Resources on Addgene.org Browse Our Special Collections Pages Suggest...

... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) who took the time...helps when the time comes to work with full force. George Church 1. “Initial and sustained motivation is ...Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions about...

...peering right through living animals to see their organs – a liver or brain, say, or maybe a tumor. They...for optical labeling and tracking of proteins, organelles, and living cells in a spatiotemporal manner,...  Kendall Morgan...

..., 641–656. https://doi.org/10.1038/s41579-022-00739-4 More resources on addgene.org  Addgene's CRISPR ...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...

...Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on Addgene.org Addgene antibodies ...Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (...

... responses. Cell Res 20, 34–50 (2010). https://doi.org/10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan...Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00022 Torres,...specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 Xu Cao, et al. Promoting...

...and space. Zebrafish have become a popular model organism because their larval stage lends itself well to...toolbox and the versatility of the zebrafish model organism, the Isacoff lab was able to link these cells ... Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral service Find...

...Searching, Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped...straight to the point. 3. Engage the reader Scientists forget that the reader is not in their head. The scientist...

... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...

...https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https://doi.org/10.1101...microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   Novel class of...

...transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535-8 Takeda S, Kadowaki S,...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...Find other fluorescent biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids...

...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

...extension and visit ReFigure.org. You can reach the ReFigure team at refigure@refigure.org Please support ... Learn more about Data Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular...

... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...Blog Posts on CRISPR Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene...