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We narrowed to 796 results for: arc

Showing: 401 - 450 of 796 results

  1. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ... red light instead of blue, which will allow researchers to independently control the activity of two .... Understanding the brain through optogenetics research Addgene: What are some of the big problems that...Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab Sign ...

  2. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Inferior: How Science Got Women Wrong - and the New Research That's Rewriting the Story by Angela Saini "This...in what happens when scientists bring biases to research and don't collaborate with others to bring diverse... series, there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...

  3. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles have some advantages...isolation protocol by Maguire and colleagues, other researchers have tried to modify it to improve yields. For... viral protocols and videos Find AAVs for your research ...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

  5. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...systems for mammalian screens, which enables researchers to maximize the chance of transducing a only ...downstream of the apoB gene. Using this reporter, the researchers explore many aspects of ApoB-LP biology, including...these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors, ...

  6. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types of biological...enough to engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly...environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department of...

  7. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...around 100-fold. These improvements will allow researchers to better fine-tune the level of output gene ... Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441...Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;...

  8. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus... 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or drive high...Cycle Popular Retroviral Vectors and Their Use in Research More resources on addgene.org Addgene Protocols...

  9. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...the University of Alberta. If you’re like many researchers these days, you are ready to take (if you haven...are often impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease”...knockout of HIV co-receptor CCR5. Nucleic Acids Research 43, 5560–5571 (2015). PubMed PMID: 25964300. PubMed...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...mutational abilities of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell...flexibility to address many different types of research questions. Are you ready to start EvolvR-ing your...Perez-Pinera. "The continuously evolving CRISPR barcoding toolbox." Genome biology 19.1 (2018): 143. PubMed...

  12. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...GPCRs. This collection, called TRUPATH, allows researchers to identify specific G proteins activated by ...s still a lot to learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic...chemogenetics guide Find chemogenetics plasmids for your research ...

  13. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...-level positions right out of college. Prior to March 2020, internships were a fiercely competitive endeavor...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ...out other tools and resources for early career researchers Applying for jobs? Check out these blog posts...

  14. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular biology and...inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM can help...Perfect Plasmid Backbone Find Plasmids for Your Research ...

  15. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...engineer and scientist, I like to envision that my research will have a direct impact on healthcare and the...result, I have occasionally pondered translating my research into a startup company. However, like many like-minded...incorporated into Novophage Therapeutics, Inc. in March 2009 and tested in the “market” by competing in ...

  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...undoubtedly constitute a valuable tool for the research community. You can find the AAV vectors for infecting...imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin and Ryohei Yasuda ...sodium/potassium pump ATP1A1 in addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations...Cre recombination. Each knockout includes unique barcodes and universal priming sites to ensure the collection...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...out plenty of roles just the way they are, the research antibodies in your refrigerator often need a little...are kind of hard to see. To solve this issue, researchers conjugate various labels to antibodies that produce...conjugation, which is commonly used for labeling research antibodies. Lysine residues are found throughout...

  18. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...low resolution and can’t be used in vivo. Some researchers have used fluorescently tagged DNA-binding proteins...CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single genomic locus,...

  19. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ... podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what... Yeast Transcription Factors Podcast Segment Researchers from the El-Samad lab recently deposited plasmids...that this level of control will enable future researchers to better simulate physiological gene expression...

  20. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...editing efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor,...

  21. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...of the different steps in RNA extraction. Researchers at the NIH in the mid 1980’s set out to develop...with chloroform as in the above no-kit method.  Researchers have also developed ways to “stabilize” RNA within...gene expression. There are several things that researchers can do to combat this issue. First and foremost...

  22. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... long tenure at Addgene. I would work with our research team and software engineers to update our Lab ...engineer that you want the LIMS software to create a barcode for your plate, you will get in trouble if you ...given container Whether or not the container was barcoded (this is pretty Addgene specific) The types or...

  24. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...this process is not fully understood, a team of researchers lead by Takayuki Kogure (Kogure et al., 2011)...

  25. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...as part of its natural viral life-cycle, and researchers have adapted the components for use in genome...-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics...parallel. Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

  26. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...provide researchers with useful and adaptable tools to perform basic and preclinical research. For scientists...Inbred and outbred strains Mouse strains used in research can fall under one of two broad classifications...

  27. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ..., Doxzen KW, Ma E, Liu J-J, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, .../10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer... on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check...

  28. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... doesn’t mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went...choice, but by circumstance. She was working as a research and technical assistant, when her life changed...often under a lot of pressure to publish their research and are not so keen on sharing their results in...

  30. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the reagents available at Addgene come from researchers, and the vast majority of our plasmids and pooled...it, there’s a reason nearly 70% of all internet searches are done via Google. The “Let me Google that for...questions, consider using Google Scholar, which searches scientific articles, patents, and even some doctoral...

  31. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...CRISPR toolbox. When Cpf1 was first discovered, researchers wondered if it could overcome some of Cas9’s ...templates should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ...possibility has not been experimentally confirmed. Researchers have also not yet characterized the potential...

  33. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... pretty good. We started doing extracurricular research on some of them to see how feasible they were,... for us? After about a month of discussion and research and lots of conversation, we hit upon the idea... They invested in us this summer – $30,000 for research and another $30,000 in mentorship and business...

  34. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic training prior...commonly administered through the tail vein — many researchers will simply refer to these as “tail vein injections...feeding tube can be flexible or metal, depending on researcher preferences. It sounds complicated but has a ...

  35. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...depositing them in Addgene’s repository. Other researchers, eager to try this new technology, were quick...the CRISPR community?  Addgene exists to help researchers share useful tools, and as such, we’re always...collection, we’ve shared almost 300,000 plasmids with researchers all over the world. That’s an average of 27,272...

  36. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... and what is their background relative to your research? Tailor your language to your audience so that...presenting at the American Association for Cancer Research Annual Meeting, you might consider including more...group who presented on a topic related to your research? Find a way to get in contact with the presenter...

  37. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your...all will be familiar with the specifics of your research. Try to avoid jargon in this initial introduction...data they may ask the audience to not share their research on social media. Use the hashtag if you are live-tweeting...

  38. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...devising their tagging strategy, another group of researchers was working on isolating monoclonal antibodies...identified oncogenes and was a target of intense research. Evan, et al. hoped that their new monoclonals...producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable domains of Myc1-...

  39. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...d like to modify. To tackle the PAM problem, researchers have engineered alternative Cas9s binding distinct...this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants with alternative...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency...

  40. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus...sybody libraries in the form of mRNA for academic research. The template plasmids for generating your own...days. While the lab used this method for COVID-19 research, this protocol holds promise for developing treatments...

  41. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library encompassing...plasmid encodes the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD...encapsulating a single enzyme. Compared with previous research, which required protein compartments to co-localize...

  42. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... essential part of being able to continue your research. As an academic postdoc, you’re often required... at once at the end of February or beginning of March, and the jobs are mixed into the general careers...developmental biology and is applying this towards researching neurodegenerative diseases.   Additional resources...

  44. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre Marillonnet share...and since we are making all the tools for plant research here and validating them, it made the most sense...academic in literature who gave a talk to the women’s research network here. She talked about the advantages ...

  45. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...variants, and their relevant mutations, check out Marcy's post from last week on GFP. Additionally, many ... & pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor...to Our Guest Blogger! Gal Haimovich, PhD, is a research fellow in the lab of Prof. Robert Singer at Albert...

  46. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...accessibility (Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural...Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses... microscopy: principles and uses in biological research. Nat Methods 16:33–41. https://doi.org/10.1038...

  47. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...delivered using this vector. For example, although researchers know that Usher Syndrome (a disease that results...encouraging. Importantly, a significant amount of basic research is being done to further improve the technology...Muzyczka N., 2014. AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014...

  48. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...technical expertise in a specific field. While searching for jobs and making connections, avoid falling...they’re seeking someone who has, to a t, all the research skills under your belt from your last job. You...managing inventories in the lab, or organizing a research project, one can assume that you’ve had to flex...

  49. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...blogger, Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...Lavis is currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would...

  50. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... by antibiotic resistant bacteria. Even when researchers develop new antibiotics, the onset of resistance...’re turning the tables on the bacteria. Future research will show if this new strategy can help us win... on Addgene.org Find CRISPR Plasmids for Your Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design...
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