We narrowed to 762 results for: SOM

... in the lab. The first post focused on streaming some lab waste for recycling- a process that’s integrated...well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability, and find...single-use is now a popular theme in life sciences, some of these plastics are high grade and can be washed...markets. Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years...practices to meet higher environmental standards. Find some easy action items on our other article in this series...

...also mutagenized proteasome subunit PSMB5 to find mechanisms of resistance to a proteasome inhibitor; CRISPR-X...bidirectional promoter, integrate at 11 specific chromosomal locations, and stably express the integrated ...however, these methods are not without limitations. Some, such as the well-known yeast two-hybrid assay are...protein interactions, whereas others may be too cumbersome to use in a high throughput manner or make it...for using the indicated antibiotics. In addition, some versions of the mAID vectors come with mCherry2 ... Yeast Vectors blog post concedes that there are some drawbacks with this technique. Great care needs ...are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for restoring...

...about “group” or “peer” mentoring? How do I ask someone to be my Mentor? I have a Mentor – what do I do... led to the topic of “What makes a good Mentor?” Some of the Tweeted comments: “A good mentor has your...mind, rather than (or as well as) their own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also...think you can finish that book in 4 weeks!)” “Sometimes a mentor is a sounding board--a good mentor asks...want to find additional sources of wisdom. While somewhat lighthearted, the article does highlight key characteristics...

...Surveyor nuclease, save some of the duplex and run it as an undigested control. Sometimes the PCR products can...add 3 microliters of 5 mM star anise?” This is something I was expecting when I set out to make my first...lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells were then used to generate a monoclonal cell...editing across all the cells in the population, so some of the cells will still appear green despite having...Bunz F. Genetic knockouts and knockins in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed...

...absence of a rigid cell wall. Here, I will cover some of the best ways to tackle mycoplasma contamination...stains like DAPI and Hoechst 33258 to stain all DNA. Some amount of expertise is required in this process ...become a habit before you even realize it. Here are some things to do: Wear a clean lab coat and mask while...culture is irreplaceable or very expensive, there are some ways to rescue the cultures. Elimination is a very...contamination and are the last alternative. There are some drawbacks while considering this treatment as the...permanent damage to the cell culture it is irreparable. Some cells may die due to longer incubation periods and...

... Project for some example proposals and consider applying for their accelerator for some hands-on guidance...about themselves--so don’t be shy and reach out! Someone from your alumni community is a great place to ... academia from graduate school or a postdoc. But some are also open to individuals from mid- to late-career...office yourself! Plus, at the federal or state level some professional societies host “Hill Days” where they...outreach groups. Grab a virtual coffee to chat with someone working in a science policy space you’re interested...

...geneticists are a lazy bunch who love to rely on the awesome power of genetic selection. Knock-in events tend...requires additional experimental manipulation. In some cases, injection of Cre is required, however the... design, delivery, screening, and more. Here are some highlights from the past few years:  Repair template...facing outwards, ensuring the PAMs remain on the chromosome after DSB formation, was most efficient (Farboud...of experiments imaginable in C. elegans. Equally, some of the “elegans” methods outlined in this post –...Wieland I, Gaul U, Forstemann K (2014) Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based...

...DNA found in bacterial cells, and someone new to plasmids may need some extra guidance to understand the...that replicate independently from the host's chromosomal DNA. They are mainly found in bacteria, but also...utilize plasmids across a wide range of applications. Some common plasmid types include cloning plasmids, expression...viral plasmids, and genome engineering plasmids. Some of the many things that plasmids can be used to ...

...current identification methods missing something? Here we will review some time-tested gene identification systems...reporter construct can be something phenotypic (such as a fluorescent protein or something species-specific) ...gene product. These are effective tools but neglect some biologically relevant situations. Tissue-specific...

...about anything can disrupt your bench work and sometimes no culprit can be found…. In our lab we call this...the lab gnome. See this video where he/she/it/something is captured on camera: Unfortunately, unless...them that they are not alone; a researcher doing some experiments in Brazil can have the same issues as...the potential contained within each. There must be some way to use LEGOs, which are practically universally...optimistically grasp at limitless potential, and who sometimes wish they could live differently than we do day...

...effectively with other teams. While they sometimes dig in and do some of the work, they must be more focused...current task. Most of us start as doers, some become managers and some take on leadership roles. You may be...promotion. Yet little explanation is given for how someone can or should demonstrate this quality.   I am ...

...everyone to attend all the meetings or that there’s some correct level of intimacy that creates the magic...live audiences in your future.     3. Start something In almost every career talk I present, I’m asked...before others do”. Candidates who have “started something” which they can share on their resumes have a ...Informational interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation...and do a character count in your head.  Here are some sites that have compiled lists of grants: Science...

...As you browse the collection, you'll likely find some proteins of interest for which you have several ...the pocket of a folded protein, or blocked by something bound to the protein, the antibody can't bind ...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...This involves using heat and/or chemicals to break some of the bonds keeping the protein folded, changing...protein and its configuration in your assay. For some assays, you may only need to validate for recognizing...

... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...viruses and focused only on those found in bacteria. Some common ones you might see include ColE1, pMB1 (which...Not all origins of replication are created equal. Some will produce many plasmid copies and others produce...antibiotic, and medium can all affect copy number. Some ori's are temperature sensitive; others ori's can...started to die off. Have any questions? Need something clarified? If so please, post in the comments....

...Before we design your first panel, we will introduce some background on the color spectrum and the principles...This step is called emission. During excitation, some of the photon's energy is lost, so the energy of...commonly used fluorophores and their emission spectra. Some fluorophores carry their emission peaks within their...However, when you need to label multiple markers, some bleeding is often unavoidable. But don't worry, ...exclude APC-Cy7 from the rest of our panel. Besides some background cell death constantly happening in cell...culture, the transduction process itself also leads to some cell death. As a result, there is a high abundance...

...2013), and visualizing structural changes in the ribosome during protein translation (Ermolenko et al., ...the same exact location as the donor. As long as some of the acceptor molecules are targeted nearby (such...synthetase/tRNA pair, a researcher can lure the ribosome into inserting a fluorophore-tagged amino acid...unnatural amino acid finds the UAG codon in the ribosome, resulting in site-specific incorporation of the...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors.... "Observation of intersubunit movement of the ribosome in solution using FRET." Journal of molecular ...

...cabinets, a rickety old incubator, a few pipettes, and some home-made equipment. It was humble in comparison...members pursue a diverse range of projects at BosLab. Some are entrepreneurs using our space to perform early-stage...probiotics to filming educational lab videos, while some are looking to develop new skill sets. Kellen Andrilenas...different samples. The goal is to not only learn something about the microbial diversity in different soil... at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement   ...

...works in a lab struggles with reproducibility at some point. Usually it comes up when a researcher decides... new project and begins by trying to reproduce someone else’s result. Then, they hit trouble. The experiment...was not, and it was just that they were doing something differently. Communication comes in many forms—from...protocols are available and detailed enough for someone else to follow them, to being open and willing ...approach to doing that will be, and it will need some effort and experimentation. But it ought to be possible...

...exported as a picture. I believe that showing something different from time to time breaks up the monologue...monologue a little and gives participants something else to focus on. Use collaborative cloud apps It is... Take brain breaks Meetings can get very long sometimes, and interaction fades away the longer we are ... hour (or another designated time) of meeting, someone could raise their hands or signal the approach ...conversations. This can help team building and bring back some degrees of belonging that was probably lost during...

...we’ll use mammalian plasmids to introduce you to some of your potential options and cloning methods, but...happen.  There are many reasons to multiplex gRNAs, some being: Using dual nickases to generate a knockout...number of gRNAs in your plasmid, you’ll need to use some assembly methods such as Golden Gate or Gibson assembly...constructed using oligonucleotide assembly.  Unlike some of the plasmids described above, this vector does...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ...Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.” Proceedings ...