...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent...

...2020). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More resources on addgene.org  View Addgene's...cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt.3290 Rozners, E. Chemical Modifications...Frontiers in Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu, Y., Wu,...Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori, E., Satyapertiwi...

...tech transfer team will communicate with your organization’s technology transfer office or other authorized...than 75 plasmids, please contact us at deposit@addgene.org. These deposits are considered large deposits... your PI and send the spreadsheet to deposit@addgene.org with the following information: Addgene account.... The name of the Principal Investigator and Organization where your plasmids were first created. From... You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which Plasmids...

... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our ...

...been an invaluable resource for studying the organization and function of cellular systems. FPs have been...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular... fluorescent protein tagging with SapTrap The Jorgensen Lab recently developed a modular plasmid assembly... References Schwartz, Matthew L. and Eric M. Jorgensen. “SapTrap, a Toolkit for High-Throughput CRISPR...Use Rosella to Monitor Autophagy Resources on Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent...

...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, Rego M, Bourges...Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné...Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia O (2011...NGS for Plasmid Quality Control Resources on Addgene.org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus...

...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein, B., &...United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang, Y., He,...Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G...microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...

...20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular...Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies...Clinical Microbiology, 23(5), 959–961. https://doi.org/10.1128/jcm.23.5.959-961.1986 Lobstein, J., Emrich...cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O., Defais,...

...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome... Appl Environ Microbiol 81:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen J, Zhang... Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036 Zhang Y, Sun X, Wang Q, Xu...Transposases. ACS Synth Biol 9:1998–2008. https://doi.org/10.1021/acssynbio.0c00073   Additional resources...all articles about microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids ...

...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...Read all of our career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our...

...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...form of figures/schematics or tables. And don’t forget to include citations so that people can go back...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info...

...Awareness Organization (ADAO) are working toward such a ban, and the president of the organization, Linda... has spread from where it originated to another organ in the body. Such misdiagnosis and late discovery...tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the surgeon ... Screening in Ghana Additional Resources on Addgene.org Check Out Our Cancer Ressearch Collection Find...

...Aspects of DNA Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano, K., & Mizobuchi.... The EMBO Journal, 17(17), 5201–5213. https://doi.org/10.1093/emboj/17.17.5201 del Solar, G., Giraldo...Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998 Grimwade, J. E.,...Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar/gky457 Park, K., Han, E., Paulsson,.... The EMBO Journal, 20(24), 7323–7332. https://doi.org/10.1093/emboj/20.24.7323 Resources at Addgene ...

...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances...gene delivery to the CNS and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene...

...construction of a so-called “genomically recoded organism” (3) but its portability to other bacterial strains...monitoring and analysing autophagy of cytosol and organelles in yeast cells. Named after the brightly-coloured... both in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly...has revolutionized genome editing in multiple organisms thanks to its simple, easily modifiable RNA-guided...screens required to identify correctly modified organisms has limited routine use of this technology. To...tag insertion in the C. elegans genome, Erik Jorgensen’s lab from the University of Utah developed SapTrap...conditionally knock down proteins in a variety of model organisms. As long as it was expressed in a cell that also...

...our days more effective, and our schedules more organized. From the alarm in the morning, to the daily news...of paper in notebooks, and hoping that we don’t forget to write things down when doing scientific research...note-taking tools, others using excel sheets or organizing folders in complex ways, there are solutions ...institution, the transition is smooth as all data is organized within an ELN already. Investing some time in ...makes it much easier for lab members to manage and organize their work at every step of the research process...

...blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156... Tags for Protein Purification Resources on Addgene.org Find Synthetic Biology Plasmids Find Bacterial...

...Alberta in Edmonton, Canada, to the University of Georgia, USA) has developed that allows for a phage genome...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...lab during its recent move to the University of Georgia). She enjoys pondering how phages get through the...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...

...couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork, and competition...will combine all of these. Addgenies have been organizing Halloween festivities for years – searching our...sweet – as sweet as all that Halloween candy you’ll gorge yourself on in the coming days. Hopefully, I’ve ...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...