Skip to main content
Addgene
Showing: 161 - 180 of 429 results

  1. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...and freely behaving mice to gather the following types of data: Report time-course and concentration of... and freely moving mice to gather the following types of data: Report endogenous DA release from a single...

  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...access unique epitopes with high affinity, due to a greater ability to target concave epitopes Suitability...

  3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...technology is versatile and can be applied across cell types and organisms by replacing the transcription factor... allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase can work in other...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified: AAV1-11. Nine... in the cap gene which differ between the AAV serotypes are responsible for serotype-specific differences...

  5. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...a marker for the mutation. When designing these types of experiments it is best practice not to use the...compatible! Check out this list of common E. coli genotypes and their natural resistances.  Additional Resources...

  6. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...deliver a genes of interest to a variety of cell types in-vivo. These are widely used by the optogenetics...revolutionized how researchers make knockouts and other types of genome edits, but, even with CRISPR, it isn’t...

  7. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...re also distributing AAV preps with particular serotypes that can be used to deliver fluorescent proteins...future users. We’re excited to be distributing new types of materials. At the time of publication we’ve already...

  9. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...antibodies within certain classification groups called isotypes.  Why does isotype matter? Isotype matters when...secondary. If you were to change one of the antibody’s isotypes, however, you could then use 2 different secondaries...

  10. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...drum. They have a wide variety of possible genome types that can be either double stranded (ds) or single...them far safer to work with than virions. The four types of viral vectors (which many researchers will informally...

  11. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression... to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV inventory for the...

  12. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... et al. tested iTOP in a series of primary cell types using Cre and various loxP reporters. In both mouse...primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal and gut...

  13. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...comparing the transduction patterns of different AAV serotypes. To help make this easier for scientists, we now...testing. These samples are available in various serotypes of two popular control AAV encoding GFP. Check...

  14. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the...blood-brain-barrier. In summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced...

  15. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether endocytosis, vesicle...explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis, vesicle...

  16. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target...systems ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription...

  17. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...Connie Cepko to learn about her management style. Recipes for delegation success Remember that there are ...authority, responsibility and accountability? What types of issues do you want to know about right away? ...

  18. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...spanning 20 years of research. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.... 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes...

  19. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... with all the new biotechnology.” These are the types of inquiries that routinely hit the inbox at the...ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

  20. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...expressing plasmid. Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope ...enables this virus to infect many different cell types so it is useful in a wide range of applications....
Showing: 161 - 180 of 429 results