We narrowed to 23 results for: HST;
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TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester...Patrick Miller-Rhodes is currently a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester...
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Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....so, you will need to include a marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for...anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified... -
New Viral Vectors - Spring 2025
TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....pAAV-S5E6-dTom-nlsdTom AAV1 Control Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog PostPublishedJan. 21, 2016, 4:52 p.m....Sample 6 website Check out Dr. Koeris' Website, biotechstart.org Read Sample 6's Paper on Bacteriophage-based... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m....does the fluorescent tag need to be pH stable or withstand a particular solute concentration or temperature... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m...., 2015). At its very core, peer-review is a touchstone of the scientific method, ensuring that published... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed. Protein... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....hfYFP is not only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay). Elizabeth Hubbard: Neurons... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m.... needed to produce 3 concurrent base edits to withstand antibiotic selection. As a result, 8 mutations... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m.... store or use. They are quite stable and will withstand freeze/thaw cycles and dehydration (Howard & Harvey... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....polymerase is thus a heat resistant enzyme that can withstand changes in temperature. Taq was first identified... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....containing vectors developed in the Jordane Dimidschsteind lab provide expression selectively in Parvalbumin... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....terms of preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve... -
Supporting Reproducibility with a Connected ELN
TypeBlog PostPublishedJan. 17, 2019, 1:38 p.m....third-party access to the ELN arises here. Notwithstanding access restrictions based on permissions, however... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....Breaks by Homologous Recombination. Jasin M and Rothstein R. Cold Spring Harb Perspect Biol (2013). PubMed... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m.... E., Dai, M., Leyva Garcia, B., Tran, T., … Dimidschstein, J., Fishell, G., Wang, Y. (2024). An enhancer-AAV... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....alternative. This method uses DNA stains like DAPI and Hoechst 33258 to stain all DNA. Some amount of expertise...