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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33 results for: HST;

Showing: 1 - 20 of 33 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester...Patrick Miller-Rhodes is currently a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...so, you will need to include a marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for...anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified...

  3. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... Thermus aquaticus (Taq) . Taq polymerase can withstand many heating and cooling cycles, which would denature...DNA polymerase: Special DNA polymerase that can withstand radical temperature changes during a typical PCR...

  6. Brain Initiative Collection

    Type
    Collection
    ...control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2-dTom-nlsdTom AAV vector...control of the E2 regulatory element Jordane Dimidschstein 135630-PHPeB pAAV-S5E2-dTom-nlsdTom AAV vector...control of the E2 regulatory element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2-GFP-fGFP AAV vector to...control of the E2 regulatory element Jordane Dimidschstein 135631-AAV9 pAAV-S5E2-GFP-fGFP AAV vector to...control of the E2 regulatory element Jordane Dimidschstein 135631-PHPeB pAAV-S5E2-GFP-fGFP AAV vector to...control of the E2 regulatory element Jordane Dimidschstein 135632-AAV1 pAAV-S5E2-GCaMP6f AAV vector to ...control of the E2 regulatory element Jordane Dimidschstein 135632-AAV9 pAAV-S5E2-GCaMP6f AAV vector to ...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed. Protein...

  11. Hot Plasmids - January 2023

    Type
    Blog Post
    ...hfYFP is not only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and...

  17. Caltech Systemic Capsids

    Type
    Collection
    ...C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein 214846...dTom-nlsdTom E2 regulatory element dTomato Control Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element...element GFP, membrane-targeted GFP Control Dimidschstein 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG...Gq-P2A-dTomato E2 regulatory element Activator DREADD Dimidschstein Biosensors 104491 pGP-AAV-syn-FLEX-jGCaMP7s-... E2 regulatory element Calcium sensor GCaMP Dimidschstein 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium...

  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...terms of preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve...
Showing: 1 - 20 of 33 results