We narrowed to 33 results for: HST;
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TypeBlog Post...contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester...Patrick Miller-Rhodes is currently a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester...
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Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...so, you will need to include a marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for...anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol... Thermus aquaticus (Taq) . Taq polymerase can withstand many heating and cooling cycles, which would denature...DNA polymerase: Special DNA polymerase that can withstand radical temperature changes during a typical PCR... -
New Viral Vectors - Spring 2025
TypeBlog Post...pAAV-S5E6-dTom-nlsdTom AAV1 Control Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog Post...Sample 6 website Check out Dr. Koeris' Website, biotechstart.org Read Sample 6's Paper on Bacteriophage-based... -
Brain Initiative Collection
TypeCollection...control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2-dTom-nlsdTom AAV vector...control of the E2 regulatory element Jordane Dimidschstein 135630-PHPeB pAAV-S5E2-dTom-nlsdTom AAV vector...control of the E2 regulatory element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2-GFP-fGFP AAV vector to...control of the E2 regulatory element Jordane Dimidschstein 135631-AAV9 pAAV-S5E2-GFP-fGFP AAV vector to...control of the E2 regulatory element Jordane Dimidschstein 135631-PHPeB pAAV-S5E2-GFP-fGFP AAV vector to...control of the E2 regulatory element Jordane Dimidschstein 135632-AAV1 pAAV-S5E2-GCaMP6f AAV vector to ...control of the E2 regulatory element Jordane Dimidschstein 135632-AAV9 pAAV-S5E2-GCaMP6f AAV vector to ... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...does the fluorescent tag need to be pH stable or withstand a particular solute concentration or temperature... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post..., 2015). At its very core, peer-review is a touchstone of the scientific method, ensuring that published... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps ... -
Another Pathway into Cells: iTOP
TypeBlog Post...osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed. Protein... -
Hot Plasmids - January 2023
TypeBlog Post...hfYFP is not only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay). Elizabeth Hubbard: Neurons... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post... needed to produce 3 concurrent base edits to withstand antibiotic selection. As a result, 8 mutations... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...polymerase is thus a heat resistant enzyme that can withstand changes in temperature. Taq was first identified... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...containing vectors developed in the Jordane Dimidschsteind lab provide expression selectively in Parvalbumin... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post... store or use. They are quite stable and will withstand freeze/thaw cycles and dehydration (Howard & Harvey... -
Caltech Systemic Capsids
TypeCollection...C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein 214846...dTom-nlsdTom E2 regulatory element dTomato Control Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element...element GFP, membrane-targeted GFP Control Dimidschstein 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG...Gq-P2A-dTomato E2 regulatory element Activator DREADD Dimidschstein Biosensors 104491 pGP-AAV-syn-FLEX-jGCaMP7s-... E2 regulatory element Calcium sensor GCaMP Dimidschstein 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...third-party access to the ELN arises here. Notwithstanding access restrictions based on permissions, however... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...terms of preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...Breaks by Homologous Recombination. Jasin M and Rothstein R. Cold Spring Harb Perspect Biol (2013). PubMed...