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We narrowed to 41 results for: Rhod

Showing: 1 - 20 of 41 results

  1. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins respond by opening and...deliver channelrhodopsins to the brain. This delivery route concentrates lots of channelrhodopsins in one...since current channelrhodopsins tools have low conductance, but also confines channelrhodopsin activation...conductance compared to current channelrhodopsins. Their new channelrhodopsins, called ChRgers, make the brain...engineering channelrhodopsins There are three main challenges to engineering channelrhodopsins to have improved...to predict if a channelrhodopsin sequence would result in a functional channelrhodopsin, as well as predict... predict which channelrhodopsins from a library of ~120,000 predicted channelrhodopsin sequences would...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins... 50 years ago with the discovery of microbial rhodopsins, light-sensitive proteins that absorb photons...light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? Neuronal stimulations...when hit with the correct wavelength, microbial rhodopsins enable ions to flow across the axon’s membrane... 2012). For example, blue light activates channelrhodopsins that, in turn, trigger neural excitation, ..., whereas yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al...genetically modified viruses encoding microbial rhodopsins into specific cerebral regions. The resulting...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ..., the JEDI-2P is an excellent choice. Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked...are two flavors of rhodopsin GEVIs. The first is a two-in-one system where rhodopsin functions as both ...second type of these GEVIs: a FP-fused rhodopsin. FP-fusion to rhodopsin resulted in electrochromic FRET capable..., with rhodopsin quenching the added fluor for this two-part fluorescent system, and rhodopsin still functioning... 2 main groups: voltage sensitive domains and Rhodopsin containing GEVIs. Chemigenetic sensors are better...exceeds that of the VSD GEVIs. However, since rhodopsin proteins were initially evolved for ion transport... functioning as the voltage sensor. FP-rhodopsin GEVIs have been engineered to have kinetics comparable...

  5. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ... of light-gated potassium channelrhodopsins, named Kalium channelrhodopsins (KCRs), from a fungus-like...-2P Novel class of light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available...   Novel class of light-gated potassium channelrhodopsins by: Mike Lacy New work from the labs of Mingshan... attempts to engineer potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm...plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural light-gated potassium channels...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Pseudomonas spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus...leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella...Rhizobium meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated cation channel...Light-gated ion channels Light activated ion channels (rhodopsins) either excite or inhibit neuronal activity upon...endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an endogenous...   Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes a conformational change when exposed...Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin-2, a directly light-gated cation-selective...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... with Mark Howarth. Shining a light on channelrhodopsins – Chronos & Chrimson Through de novo sequencing...Boyden’s lab has identified a variety of new channelrhodopsins for use as optogenetics tools. These tools...tools include a pair of channelrhodopsins, Chronos and Chrimson, which have enabled the group to perform ...The paper describes five previously unknown channelrhodopsins from different species: Stigleoclonium helveticum...and has faster kinetics than those of other channelrhodopsins. CnChR1, nicknamed Chrimson, is the first ...first reported yellow-peaked channelrhodopsin with a spectral peak at 590 nm, which is 45 nm more red-shifted...

  9. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex...For example, the light-gated cation channel, Channelrhodopsin 2 (ChR2), exists in several states: closed...coordination by Gi/o protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological...PMID: 22157972. 3. Britt JP, et al. Use of channelrhodopsin for activation of CNS neurons. Current protocols...

  10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... classic small molecule fluorophores, such as rhodamines (Figure 2a), were discovered in the 19th century...structure have yielded commercial panels of advanced rhodamine fluorophores, such as the Alexa Fluor and ATTO... brightness and photostability of the classic rhodamines and their analogs without sacrificing their small...advanced imaging modalities. Follow him on twitter @rhodamine110.   References 1. Liu, Zhe, Luke D. Lavis, and...

  11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific...and subsequent firing of an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as...PMC3337962. 8. Zhao S, et al. Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection...

  12. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma #49004) to the spectra of both...mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter wavelength...filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser...

  13. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...metagenomically discovered marine anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion... step-function artificial anion conducting channelrhodopsin (aACR). It is activated by cyan light and ...

  14. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent protein (mScarlet...fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N quenches a proportion...green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids...

  15. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...Patrick Miller-Rhodes from the University of Rochester Medical Center. Patrick Miller-Rhodes is currently...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ..., Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use...plasma membrane. To obtain a more sensitive channelrhodopsin actuator for triggering action potentials ...lab further modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified by Ed...

  17. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ... illustrated with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being...Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast, Tim also hosted me at his University...

  18. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate...
Showing: 1 - 20 of 41 results