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We narrowed to 50 results for: Rhod

Showing: 1 - 20 of 50 results

  1. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post
    ... shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins respond by opening and...deliver channelrhodopsins to the brain. This delivery route concentrates lots of channelrhodopsins in one...since current channelrhodopsins tools have low conductance, but also confines channelrhodopsin activation...conductance compared to current channelrhodopsins. Their new channelrhodopsins, called ChRgers, make the brain...engineering channelrhodopsins There are three main challenges to engineering channelrhodopsins to have improved...to predict if a channelrhodopsin sequence would result in a functional channelrhodopsin, as well as predict... predict which channelrhodopsins from a library of ~120,000 predicted channelrhodopsin sequences would...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post
    ...Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins... 50 years ago with the discovery of microbial rhodopsins, light-sensitive proteins that absorb photons...light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? Neuronal stimulations...when hit with the correct wavelength, microbial rhodopsins enable ions to flow across the axon’s membrane... 2012). For example, blue light activates channelrhodopsins that, in turn, trigger neural excitation, ..., whereas yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al...genetically modified viruses encoding microbial rhodopsins into specific cerebral regions. The resulting...

  4. Viral Vectors 101: Voltage Indicators

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    Blog Post
    ..., the JEDI-2P is an excellent choice. Rhodopsin GEVIs Rhodopsin is composed of an opsin (a GPCR) linked...are two flavors of rhodopsin GEVIs. The first is a two-in-one system where rhodopsin functions as both ...second type of these GEVIs: a FP-fused rhodopsin. FP-fusion to rhodopsin resulted in electrochromic FRET capable..., with rhodopsin quenching the added fluor for this two-part fluorescent system, and rhodopsin still functioning... 2 main groups: voltage sensitive domains and Rhodopsin containing GEVIs. Chemigenetic sensors are better...exceeds that of the VSD GEVIs. However, since rhodopsin proteins were initially evolved for ion transport... functioning as the voltage sensor. FP-rhodopsin GEVIs have been engineered to have kinetics comparable...

  5. Hot Plasmids - October 2022

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    Blog Post
    ... of light-gated potassium channelrhodopsins, named Kalium channelrhodopsins (KCRs), from a fungus-like...-2P Novel class of light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available...   Novel class of light-gated potassium channelrhodopsins by: Mike Lacy New work from the labs of Mingshan... attempts to engineer potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm...plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural light-gated potassium channels...

  6. Brain Armamentarium

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    Collection
    ...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer...mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer...

  7. Optogenetics Plasmid Collection

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    Collection
    ...opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps that can ... as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ...cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified to increase...

  8. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...Pseudomonas spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus...leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella...Rhizobium meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae...

  9. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated cation channel...Light-gated ion channels Light activated ion channels (rhodopsins) either excite or inhibit neuronal activity upon...endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an endogenous...   Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes a conformational change when exposed...Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin-2, a directly light-gated cation-selective...

  10. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ... with Mark Howarth. Shining a light on channelrhodopsins – Chronos & Chrimson Through de novo sequencing...Boyden’s lab has identified a variety of new channelrhodopsins for use as optogenetics tools. These tools...tools include a pair of channelrhodopsins, Chronos and Chrimson, which have enabled the group to perform ...The paper describes five previously unknown channelrhodopsins from different species: Stigleoclonium helveticum...and has faster kinetics than those of other channelrhodopsins. CnChR1, nicknamed Chrimson, is the first ...first reported yellow-peaked channelrhodopsin with a spectral peak at 590 nm, which is 45 nm more red-shifted...

  11. The Materials Science of Optogenetics Experiments

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    Blog Post
    ...stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex...For example, the light-gated cation channel, Channelrhodopsin 2 (ChR2), exists in several states: closed...coordination by Gi/o protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological...PMID: 22157972. 3. Britt JP, et al. Use of channelrhodopsin for activation of CNS neurons. Current protocols...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post
    ... classic small molecule fluorophores, such as rhodamines (Figure 2a), were discovered in the 19th century...structure have yielded commercial panels of advanced rhodamine fluorophores, such as the Alexa Fluor and ATTO... brightness and photostability of the classic rhodamines and their analogs without sacrificing their small...advanced imaging modalities. Follow him on twitter @rhodamine110.   References 1. Liu, Zhe, Luke D. Lavis, and...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post
    ...standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific...and subsequent firing of an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as...PMC3337962. 8. Zhao S, et al. Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection...

  14. Brain Initiative Collection

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    Collection
    ...AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and ...AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond... pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single millisecond...pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted...

  15. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post
    ...Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma #49004) to the spectra of both...mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter wavelength...filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser...

  16. AAV Viral Preps

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    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

  17. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post
    ...metagenomically discovered marine anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator...Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin converted into a chloride-conducting anion... step-function artificial anion conducting channelrhodopsin (aACR). It is activated by cyan light and ...

  18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red fluorescent protein (mScarlet...fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N quenches a proportion...green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids...

  19. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...Patrick Miller-Rhodes from the University of Rochester Medical Center. Patrick Miller-Rhodes is currently...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...

  20. Fluorescent Protein Guide: Biosensors

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    Collection
    ...Yulong Li ADP Biosensor for ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless ...reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25...reporter VARNAM Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator. Neuron. 2023... mammalian neurons using engineered microbial rhodopsins. Nat Methods. 2014 Aug;11(8):825-33. Adam Cohen...D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools for use in muscles...
Showing: 1 - 20 of 50 results