CRISPR 101: Ribonucleoprotein (RNP) Delivery
Type
Blog Post
Published
Sept. 6, 2018, 12:02 p.m.
... with new techniques rapidly being developed. Performing a CRISPR experiment requires delivery of, at ...your genomic site of interest. This is commonly performed by transfecting cells with a plasmid, such as ...Cas9-gRNA ribonucleoproteins
An advantage of performing CRISPR experiments using RNPs is the variety ... RNP. This advantage has allowed scientists to perform in vitro and in vivo CRISPR studies in experimental...PEG-mediated methods, while technically less difficult to perform, often show low efficiency, while biolistic bombardment...plasmid based systems, most notably the ability to perform CRISPR studies in cells that are difficult to transfect...these have been acquired, genomic editing can be performed rapidly and, in many cases, with reduced chance...