Optogenetics + CRISPR, Using Light to Control Genome Editing
Type
Blog Post
Published
Sept. 3, 2020, 12:15 p.m.
...photoactivatable system to cleave a target DNA sequence (Nihongaki, et al., 2015) resulting in a double strand break...might expect from the modular nature of Cas9, Nihongaki et al. showed that it was possible to swap out...
Split Systems / Light-induced Dimerization
Nihongaki et al., 2015
CRY2 and CIB1 from A. thaliana
...thaliana
dCas9
Activation of gene transcription
Nihongaki et al., 2015
paCas9
Magnet proteins from N. crassa...HDR, repression of gene expression by CRISPRi
Nihongaki et al., 2017
Split-CPTS2.0
CRY2 and CIB1 from ...6289 . https://doi.org/10.1002/anie.201900788
Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto ...:963–966 . https://doi.org/10.1038/nmeth.4430
Nihongaki Y, Kawano F, Nakajima T, Sato M (2015) Photoactivatable...