... Cre/lox recombination system. A researcher can flank fluorescent proteins in a plasmid with loxP sites...respectively. The permanent labelling of cells using cre-lox is advantageous when compared to photoactivable or...

...for use in genome engineering, including the Cre-lox system from the P1 phage of E. coli. Fun fact: There...

...Resources on the Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Interview: ...

...expression systems exist (eg. LexA/LexAop and Cre/Lox systems) in case you want to express one gene in ...

...good, the Cre-Lox system offers inducible yet permanent gene excision. Essentially, LoxP sites are knocked...time-tested system but does require knock-in of the LoxP sites, which is more difficult than a simple knock...out plasmids? Check out our Cre vector and Murine LoxP vector.   Turn it off for a little while Transient...

...Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with GFP Resources at Addgene.org...

...lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, insect expression...

...Specific Recombination in Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids...

...Delivery to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene...

...orange) (Livet et al., 2007). Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent...Brainbow 2.1 construct in the Rosa26 locus, under a loxP “roadblock” so that XFP are only expressed following...

... safe for clinical applications. Excisable Cre-lox lentiviral vectors: This approach is the same as ...transgenes that are flanked by loxP sites. While there is only a small loxP scar behind at the site of excision...

... involving recombination elements such as FRT or Lox sites, and provides a modular system for easier exchange...recombination events at the genetic level. Upon Cre/loxP recombination, each transgene expresses one of three...recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems...

...Both loxP and lox511 are recognized by Cre but lox511 sites can only recombine with other lox511 sites... the identical loxP or lox511 sites (Figure 1, bottom). Since only one loxP and lox511 site will remain...with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and lox511...binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in ...one wild-type pair (loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads)...sites, not with loxP sites. Alternatively, you could use a pair of loxP sites and a pair of FRT sites and... proceed either by first utilizing the loxP sites or the lox551 sites. Regardless, the first recombination...

...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...original design for creating a conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting... and two repair templates in order to insert two loxP sites. Since two double-strand breaks are made, ...variety of other undesired outcomes, including one loxP site insertion and one NHEJ-repaired indel, often... - 0.8 kb apart. In the example below, we’ll add loxP sites for conditional deletion of exon 6 of Syt1...homology on each side. For optimal repair, place the loxP sites precisely at the cut site for each guide. ...efficiency of Easi-CRISPR for simple knock-ins and floxed models varies from 8.5-100% of pups born, with ...

...technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site is left...Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides...

...easy to remove after injection, they added flanking loxP sites and a heat-shock inducible Cre to generate...schematic below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only...

...pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP ... mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP ...

...GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The...)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE...

... of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred vector...Then, if necessary for subsequent experiments, the floxed neoR gene is removed via infection with adeno-cre...

...Lab used this backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus, allowing for...Commonly used sequences, including fluorescent tags, a floxed Cbr-unc-119 selectable marker, and “connectors”...