Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Showing: 21 - 40 of 164 results
  1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...fluorescent proteins, and luminescent proteins. While fluorescent proteins and luminescence have been widely used... fluorescence or luminescence, which require UV lamps, fluorometers, or luminometers, chromoprotein detection...striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful corals, to name...
  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... dyes are now commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific...Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc...Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly...permeabilized cells  Cy5NTAc 43 34-62 YFP   Lumio Red Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly...
  3. Quick New Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...plasmid information as outlined by the column headers (required columns are shown in red in the spreadshet...your plasmids with the scientific community. Column Title Description Plasmid Name The name of the...other, the species can be indicated in a separate column. Relevant Mutations Enter any mutations in ...
  4. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...vivo.  Read on to learn more about this killer illumination technique! What is a photosensitizer? First...within 30 minutes of exposure. 90 seconds of illumination were sufficient for 100% effective killing of...the dye. Embryos exposed to just 12 minutes of illumination had fewer mCer3-labeled cells in their hearts...photosensitizer protein that generates ROS when illuminated with green light. They found that KillerRed required...
  5. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...the volume typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture volume into...resuspension of the collected particles in a smaller volume. Always remove packaging cell debris before proceeding...without ultracentrifugation, you can reduce the volume of cell culture medium on the packaging cells immediately...
  6. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...are both great options for this, depending on the volume of culture you chose to work with. You will get... construct at a time. For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium strains...messy, so you want to make sure you have sufficient volume to dip all your plants. Make sure your Agrobacterium... viable. The time this will take depends on the volume of culture you are using and whether you are inoculating...
  7. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Nickel Column: Lyse the bacteria expressing your nanobody and run the lysate over a nickel column. Nanobodies...Nanobodies will bind to the column due to their N-terminal His14-bdNEDD8 tag. After several washes, this...protease, thus releasing purified nanobodies from the column. bdNEDP1 protease can be generated using plasmid...
  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...solution to avoid autofluorescence is offered by bioluminescent proteins. These probes generate light by a ...there is no need to excite the sample. Although bioluminescent probes are usually dim relative to fluorescent...palette of Nano-lanterns for real-time multicolor luminescence imaging." Proceedings of the National Academy...PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of deep tissue in freely moving animals...
  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Bioluminescent pH sensor Understanding intracellular pH regulation...pRSETb-pHlash and pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed...simultaneously acquired from the the fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio...voltage sensitivity and speed, and an endoplasmic reticulum export motif and trafficking sequence for improved...dynamics and interactions between the endoplasmic reticulum (ER), mitochondria, cytoskeleton, and endosomes...PMID: 26456817. Nano-lanterns for multicolor luminescence imaging  Fluorescent imaging techniques have...The Nano-lanterns rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons...
  10. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...the plasmid DNA from the column using a particular buffer or water. See column manufacturers for more detail...DNA; filter the remaining plasmid DNA through a column that binds the plasmid DNA and lets other materials... detail. Find columnless purification protocols on our website. 6. Verify the plasmid After purifying ...
  11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light (~400 nm), the chromophore...PA-GFP to display almost no fluorescence when illuminated with 488nm light. However, when UV-violet light... to a 100-fold increase in fluorescence when illuminated with blue light (1). Similar to PA-GFP, scientists...derived from DsRed and mRFP, respectively. When illuminated with violet light, both variants are photoconverted...
  12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...chosen focal volume and thus the microscope captures only fluorescence coming from this volume. The consequence...organism to make the complicated transition from a clump of cells to a fully differentiated body. In contrast...of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement." ...
  13. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ... the sequence of interest. 5. Use a silica spin column for purification of the samples you send for DNA...isopropanol precipitation method or using a silica spin column available from several vendors. The precipitation... I would strongly recommend using a silica spin column. They can be pricey, but well worth it. 6. Edit...
  14. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...descending order, in the case of numeric columns.  The data in any column are searchable and you can search ...You can access the datatable here. Current table columns are target gene, species, sequence, Addgene plasmid...
  15. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ... sharing concerns. Ask alumni of the lab. Most labs have websites listing alumi and it is easy to use ...ignore warning signs If lab members, neighbors or alumni give you information about the PI that concerns...
  16. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...contributed by Theresa Liao of the University of British Columbia. When transitioning from an academic science career...Good luck! Resume 101 from University of British Columbia, Student Services - includes videos on general... of Physics & Astronomy, University of British Columbia. Originally on track for a research career, she...
  17. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...locally. Your university probably has an alumni directory. Alumni who enter their information in these searchable...information so you can tap them for advice. As a future alum, these are people you can “cold call” and ask for...
  18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...plexWell technology to easily and quickly create Illumina sequencing libraries. From start to finish the...libraries are then QC’d, pooled, and prepared for Illumina sequencing on our newly donated MiSeq.  For Complete... also adds the necessary adapter sequences for Illumina sequencing as well as unique barcodes per sample...
  19. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...saturation density, decreased rates of growth, or clumping. To ensure the best possible results for any tissue...approach is that it tends to result in a higher volume of lower titer virus. In such cases you may want...them! Changes in such variables as cell density, volume, incubation time, and MOI can all impact the success...
  20. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...what if an FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm...control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also... inheritance independently of the endoplasmic reticulum." The Journal of cell biology 154.2 (2001): 317...
Showing: 21 - 40 of 164 results