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Showing: 41 - 60 of 193 results
  1. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...contributed by Theresa Liao of the University of British Columbia. When transitioning from an academic science career...Good luck! Resume 101 from University of British Columbia, Student Services - includes videos on general... of Physics & Astronomy, University of British Columbia. Originally on track for a research career, she...
  2. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...locally. Your university probably has an alumni directory. Alumni who enter their information in these searchable...information so you can tap them for advice. As a future alum, these are people you can “cold call” and ask for...
  3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...plexWell technology to easily and quickly create Illumina sequencing libraries. From start to finish the...libraries are then QC’d, pooled, and prepared for Illumina sequencing on our newly donated MiSeq.  For Complete... also adds the necessary adapter sequences for Illumina sequencing as well as unique barcodes per sample...
  4. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology...need to modernize our approach to handle larger volumes of data. In this two-part blog series, we will ...continuous updates. The generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary...
  5. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...what if an FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm...control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also... inheritance independently of the endoplasmic reticulum." The Journal of cell biology 154.2 (2001): 317...
  6. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...saturation density, decreased rates of growth, or clumping. To ensure the best possible results for any tissue...approach is that it tends to result in a higher volume of lower titer virus. In such cases you may want...them! Changes in such variables as cell density, volume, incubation time, and MOI can all impact the success...
  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...barcoded, locus-specific libraries compatible with Illumina next generation sequencing in order to study evolutionary..., with each plasmid carrying a unique barcoded Illumina-M13F bridging primer. These bridging primers are... are amplified and used to generate Illumina sequencing libraries using a 2-step PCR-based protocol and...
  8. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...  Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends ... through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain cell...
  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...injections are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis et...parentheses in the"Mouse Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-...transducing the macaque brain, except for the cerebellum, brain stem, and spinal cord. All PALs, including...compared to AAV9 in the cerebrum, but not the cerebellum, in macaques. It also effectively transduces ...
  10. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment... the equation is labeled with its corresponding column in the calculator spreadsheet: We hope that this...
  11. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...Red2Green, a program that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack...this material to produce various grades of plastic lumber and distribute it to industrial and residential...
  12. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...
  13. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... require injection of substrate to produce bioluminescence. Substrate pharmacokinetics and biodistribution...localization: In vivo imaging with fluorescent or bioluminescent reporters usually only detects the signal of...
  14. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... The amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...
  15. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to ...electrical activity through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used...
Showing: 41 - 60 of 193 results