... from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins....Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which can be purified (along with...with its fusion protein) with amylose resin. The MBP tag, although large, can actually increase expression...which can be isolated with glutathione resin. Like MBP, it can be removed via protease cleavage at sites...sites immediately following the tag. Unlike MBP, the GST tag can be cleaved off from the fusion protein while...fusion proteins from being purified by GST (maybe try MBP if you are having solubility issues!). Need a GST...

...interest fused to maltose binding protein (MBP, New England BioLabs). MBP has a similar function as GST in that...the fusion protein from a bacterial extract. The MBP tag can be removed using Xa protease. The carboxyl-terminus...AB3596) and mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor Xa loves calcium...

...genes over time, at a range of distances from 2-75 Mbp. Labeling multiple, intrachromosomal loci Building...resolved loci with physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating...established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that they could evaluate the ...

...resolved loci with physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating...established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that they could evaluate the ...

...and stabilization, as can maltose-binding protein (MBP). Overexpression can also lead to insolubility, and... and aggregated protein is not useful protein. MBP tags can help with solubility issues, but scientists...(Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and Purification Can...

...peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal ...

...enable affinity purification using a Ni-NTA resin. MBP improves protein solubility and stability. TRX and...

...TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like nonstructural...

...related hybrid mice narrowed the search to a ~4.5 Mbp region of the mouse chromosome. Of the genes located...

...Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N terminal); 6xHis (N terminal); 2xNLS (C terminal...

...), gRNAs used for TZAP gene editing, and a Hi6xs-MBP tagged TZAPznf9-11constructs used for bacterial expression...

...including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, solubilization...

...first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.) Since then, we've distributed...

...with a specific resin (e.g. 6xHis containing pDEST-HisMBP). After purification the protein can be released...

...widening the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids...

...p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian Cells pCMV-DnpK...

...fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher construct. This construct is used to ...