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We narrowed to 18 results for: mbp

Showing: 1 - 18 of 18 results

  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ... from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins....Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which can be purified (along with...with its fusion protein) with amylose resin. The MBP tag, although large, can actually increase expression...which can be isolated with glutathione resin. Like MBP, it can be removed via protease cleavage at sites...sites immediately following the tag. Unlike MBP, the GST tag can be cleaved off from the fusion protein while...fusion proteins from being purified by GST (maybe try MBP if you are having solubility issues!). Need a GST...

  2. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...interest fused to maltose binding protein (MBP, New England BioLabs). MBP has a similar function as GST in that...the fusion protein from a bacterial extract. The MBP tag can be removed using Xa protease. The carboxyl-terminus...AB3596) and mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor Xa loves calcium...

  3. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...genes over time, at a range of distances from 2-75 Mbp. Labeling multiple, intrachromosomal loci Building...resolved loci with physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating...established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that they could evaluate the ...

  4. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...resolved loci with physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating...established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that they could evaluate the ...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...and stabilization, as can maltose-binding protein (MBP). Overexpression can also lead to insolubility, and... and aggregated protein is not useful protein. MBP tags can help with solubility issues, but scientists...(Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and Purification Can...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP signal ...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...), gRNAs used for TZAP gene editing, and a Hi6xs-MBP tagged TZAPznf9-11constructs used for bacterial expression...

  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, solubilization...

  13. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.) Since then, we've distributed...

  14. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...with a specific resin (e.g. 6xHis containing pDEST-HisMBP). After purification the protein can be released...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came on March...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher construct. This construct is used to ...
Showing: 1 - 18 of 18 results