Antibodies 101: Affinity Tags
Type
Blog Post
... from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins....Maltose binding protein
Maltose binding protein (MBP) is a 45 kDa tag which can be purified (along with...with its fusion protein) with amylose resin. The MBP tag, although large, can actually increase expression...which can be isolated with glutathione resin. Like MBP, it can be removed via protease cleavage at sites...sites immediately following the tag. Unlike MBP, the GST tag can be cleaved off from the fusion protein while...fusion proteins from being purified by GST (maybe try MBP if you are having solubility issues!).
Need a GST...