CRISPR 101: Any Base Transversion Editors
Type
Blog Post
Published
April 1, 2025, 1:15 p.m.
...).
gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G base from DNA to create...inosine from DNA: N-methylpurine DNA glycosylase (MPG), also known as alkyladenine DNA glycosylase (AAG...developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency, but it didn’t ...researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow it to act on newly-deaminated...hypoxanthine + a ribose sugar.
Their fusion of wild-type MPG to an ABE was able to edit A → Y (Y = T or C) in ...thereafter, using mouse instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al., 2024...deaminase-free base editor when they realized that MPG could be engineered to act directly on unmodified...