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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16 results for: pb

Showing: 1 - 16 of 16 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...for therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element ...” mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is...interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, ...-expressing the transposase. A challenge of using PB to generate iPSCs is that genomic alterations can...

  2. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...for 5–10 minutes 2–3x in fresh wash buffer such as PBS or TBST. Stringent stripping The stringent stripping...for 5–10 minutes 2–6x in fresh wash buffer such as PBS or TBST. Note that βME reacts with antibodies, so...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... gRNA extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase... for optimizing your pegRNA primer binding site (PBS) and reverse transcriptase template (RTT). Created...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline), that comes in contact...

  9. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All...

  10. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...vectors: progress and applications. Banasik MB, McCray PB Jr. Gene Ther. 2010 Feb;17(2):150-7. PubMed PMID:...

  12. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold more active in mammalian...

  13. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion constructs...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...blockers include dry non-fat milk or serums like FBS, PBS, and BSA. For nitrocellulose membranes, which require...
Showing: 1 - 16 of 16 results