...for therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element ...” mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is...interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, ...-expressing the transposase. A challenge of using PB to generate iPSCs is that genomic alterations can...

...for 5–10 minutes 2–3x in fresh wash buffer such as PBS or TBST. Stringent stripping The stringent stripping...for 5–10 minutes 2–6x in fresh wash buffer such as PBS or TBST. Note that βME reacts with antibodies, so...

... gRNA extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase... for optimizing your pegRNA primer binding site (PBS) and reverse transcriptase template (RTT). Created...

...https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L...

...reporter. Lastly, once a mutant is identified, the PB transposase can easily and seamlessly remove the ...

...Inheritance Amazon Ghost in your genes documentary PBS Additional Resources at Addgene Find the Ting Lab...

...can then be rinsed and resuspended in cold sterile PBS.  Alternatively, to concentrate a virus solution ...

...means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline), that comes in contact...

...containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All...

...vectors: progress and applications. Banasik MB, McCray PB Jr. Gene Ther. 2010 Feb;17(2):150-7. PubMed PMID:...

...cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold more active in mammalian...

...for base editing References Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ,...

...DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion constructs...

...prime editor; RTT = reverse transcriptase template; PBS = primer binding site. Image from Doman et al., 2023...

...blockers include dry non-fat milk or serums like FBS, PBS, and BSA. For nitrocellulose membranes, which require...

...Tris-buffered saline (TBS) or phosphate-buffered saline (PBS) are the most commonly used solutions for washing...