...0.1% in PBS: 49.5 mL PBS + 500 µl Pluronic F68 B: 0.01% in PBS: 45 mL PBS + 5 ml A C: 0.001% in PBS: 45 mL...Pluronic-F68 in PBS (A) Add 500 µL of 10% Pluronic-F68 to 49.5 mL PBS 0.01% Pluronic-F68 in PBS (B) Add 5 mL...syringe 18 ga blunt edge needles, Hamilton 1X PBS pH 7.4 1X PBS-MK buffer 100X Pluronic-F68 NaCl MgCl 2 KCl... M NaCl/PBS-MK buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final...store at 4 °C. 1X PBS-MK buffer Dissolve 26.3 mg of MgCl 2 , and 14.91 mg of KCl in 1× PBS in a final volume... mL of Buffer A to 45 mL PBS 0.001% Pluronic-F68 in PBS + 200 mM NaCl (C) (formulation buffer) Add 5 mL...of 1 M NaCl/PBS-MK buffer 25% iodixanol step: mix 5 mL of 60% iodixanol and 7 mL of 1X PBS-MK buffer and...

...-100 in 10 mL PBS. Blocking buffer: Dilute 0.5 g BSA and 30 µL Triton X-100 in 10 mL PBS. Antibody dilution...pipette 200–1000 µL single channel pipette Reagents 1X PBS Microcentrifuge tubes Sterile Poly-D-lysine coated...Dilute 0.5 g BSA and 150 µL Triton X-100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 µM DAPI...diluting 2.1 µL of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working...5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding...the 24-well plate. Wash each well with 500 µL of PBS, remove the wash, and dispose of it in an appropriate... well with 500 µL of cold 4% paraformaldehyde in PBS on ice for 15 min . Pro-Tip While 4% Paraformaldehyde...

...900 µL PBS in a microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into 450 uL PBS in a microfuge... µL PBS in a microfuge tube and vortex. 0.25 ng/µL : Add 450 µL of 0.5 ng/µL stock into 450 µL PBS in ...ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0 ng/µL : 450 µL PBS only. Pipette 50 µL into....05% Tween-20 in PBS) as follows: Pipette 0.5 mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and...blocking buffer (1% BSA in PBS) as follows: Add 250 mg of BSA to 25 mL of PBS. Cap the tube and invert ...dilution buffer (0.1% BSA in PBS) as follows: Dilute 50 mg BSA into 50 mL PBS . Cap the tube and invert ...89130-898 1 L polystyrene bottle, Corning 430518 PBS, 1X pH 7.4, VWR 45000-446 TMB (3,3',5,5'-tetramethyl...

... isotype standard in PBS as follows: 1.5 mg/mL: 18 µL of 2.5 mg/mL with 12 µL PBS 1.0 mg/mL: 20 µL of ...of 1.5 mg/mL with 10 µL PBS 0.75 mg/mL: 15 µL of 1.0 mg/mL with 5 µL PBS 0.5 mg/mL: 13.3 µL of 0.75 mg/...mg/mL with 6.7 µL PBS 0.25 mg/mL: 10 µL of 0.5 mg/mL with 10 µL PBS 0.125 mg/mL: 10 µL of 0.25 mg/mL with...with 10 µL PBS Transfer 5 µL of each standard to a microfuge tube. Add 8 µL of PBS to a total volume of...Corning 4853 Microcentrifuge tubes, Neptune 3745.X PBS, 1X pH 7.4, VWR 45000-446 250 mL sterile bottles,...recombinant antibody to a microfuge tube. Add 8 µL of PBS to total 13 µL . Prepare 1X MOPS buffer Dilute 25...

...container. Add 5 mL PBS to the column. Centrifuge at 1000 x g for 2 min to remove PBS. Repeat Steps 30–31...GE18-3216-03 5% Sodium Azide, 500 mL, VWR 7144.8-16 PBS, 1X pH 7.4, VWR 45000-446 Benzamide, Millipore Sigma...sample volumes <1.5 mL top off the sample with 0.2 mL PBS to aid protein recovery. Allow the resin bed to fully...Spectrophotometer. Dilute antibody to 1 mg/mL with PBS if needed. For long term storage, add sterile sodium...concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-4 30 kDa MWCO ...column. Fill the remaining space in the column with PBS and pipette several times to mix. Centrifuge at 3100...container. Fill the remaining space in the column with PBS and pipette several times to mix. Centrifuge at 3100...

...culture media and rinse once with 10 mL of PBS. Aspirate PBS and add 2 mL of 0.05% Trypsin/EDTA. Wait ~...Aspirate culture media and rinse once with 60 mL PBS. Aspirate PBS and add 35 mL of 0.05% Trypsin/EDTA. Wait ...Trypsin/EDTA (e.g. TrypLE, Thermo Fisher, 12605010) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040...mL conical vessels. Rinse CS5 once with 100 mL of PBS and add to the harvested media and cells. Centrifuge...

...FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040...from the wells. Gently wash the cells with 1 mL of PBS and aspirate wash. Filter 0.1% crystal violet solution...Gently remove the stain. Wash cells 3x with 1 mL of PBS, being careful not to disturb the colonies. Count...

... FBS Polybrene (10mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040.... Gently aspirate media and replace with 1 mL of PBS. Calculate the fraction of fluorescent-positive cells...

...glutamine such as glutaGRO, Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040...pipette for each well. Wash wells with 1 mL of 1X PBS and aspirate, using a new aspirating pipette for ...aspirate supernatant. Wash cell pellets in 500 µL PBS. Centrifuge for 5 min at 100 x g . Gently aspirate...

... Cold room Gel imager -80 °C freezer Reagents 1X PBS Lysis buffer e.g., RIPA lysis buffer Microcentrifuge...supernatant. Resuspend cell pellet in 1 mL of 1X PBS and transfer to a microcentrifuge tube. Centrifuge...

...Polyethylenimine, linear MW 25,000 Da Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium, Corning 21-040...

...FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040...

...glutaGRO, Corning 25-015-CI) Heat-inactivated FBS 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040...