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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 36 results for: pb

Showing: 1 - 20 of 36 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...for therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element ...” mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is...interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, ...-expressing the transposase. A challenge of using PB to generate iPSCs is that genomic alterations can...

  2. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...for 5–10 minutes 2–3x in fresh wash buffer such as PBS or TBST. Stringent stripping The stringent stripping...for 5–10 minutes 2–6x in fresh wash buffer such as PBS or TBST. Note that βME reacts with antibodies, so...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an RT template. During ...and generates a 3’ flap. The 3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated... Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No mCherry Ervin Welker 177181 pDAS12222_U6-pegRNA-BFP... Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No BFP Ervin Welker Do you have suggestions for...

  4. Design Tips for Prime Editing

    Type
    Blog Post
    ... gRNA extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase... for optimizing your pegRNA primer binding site (PBS) and reverse transcriptase template (RTT). Created...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...Myc for iPS cell generation Tarjei Mikkelsen 172115 PB-TO-hNGN2 Piggybac Tet-On plasmid for differentiating...

  11. Retrovirus Plasmids

    Type
    Collection
    ...Packaging Contains MoMLV gag, pol, and env Verma 35614 pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454...

  12. Viral Production at Addgene

    Type
    Blog Post
    ...containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All...
Showing: 1 - 20 of 36 results