...for therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element ...” mechanism between a vector and chromosomal DNA. PB has a cloning capacity of ~9-14 kb. This system is...interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following reprogramming, ...-expressing the transposase. A challenge of using PB to generate iPSCs is that genomic alterations can...

...for 5–10 minutes 2–3x in fresh wash buffer such as PBS or TBST. Stringent stripping The stringent stripping...for 5–10 minutes 2–6x in fresh wash buffer such as PBS or TBST. Note that βME reacts with antibodies, so...

...longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an RT template. During ...and generates a 3’ flap. The 3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated... Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No mCherry Ervin Welker 177181 pDAS12222_U6-pegRNA-BFP... Mammalian hU6 pegRNA BpiI for pegRNA, Esp31 for PBS-RT No BFP Ervin Welker Do you have suggestions for...

... gRNA extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase... for optimizing your pegRNA primer binding site (PBS) and reverse transcriptase template (RTT). Created...

...https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L...

...reporter. Lastly, once a mutant is identified, the PB transposase can easily and seamlessly remove the ...

...be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under...

...Inheritance Amazon Ghost in your genes documentary PBS Additional Resources at Addgene Find the Ting Lab...

...can then be rinsed and resuspended in cold sterile PBS.  Alternatively, to concentrate a virus solution ...

...means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline), that comes in contact...

...containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All...

...vectors: progress and applications. Banasik MB, McCray PB Jr. Gene Ther. 2010 Feb;17(2):150-7. PubMed PMID:...

...Packaging Contains MoMLV gag, pol, and env Verma 35614 pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454...

... ligand binding domain CMV Mammalian Green 12529 pBS MCK CRE Muscle-specific expression of Cre MCK Mammalian...photorecetor cells in the retina Nrl Mammalian Cepko 13962 pBS mPrx1 Cre (CT#644) Cre; expression in developing ...Tamoxifen inducible Hsp68 Mammalian Melton 15508 pBS-OMP-CreFNF OMP targeting vector with Cre FNF (neo-selectable... flanked by FRT sites) Mammalian Mombaerts 15509 pBS-M71-IRES-CreFNF M71 targeting vector with IRES CreFNF...

...F68 or 0.014% Tween 20. Phosphate Buffered Saline (PBS) A universal buffer suitable for any application ...

...for base editing References Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ,...

...cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold more active in mammalian...

...DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion constructs...

...prime editor; RTT = reverse transcriptase template; PBS = primer binding site. Image from Doman et al., 2023...

...blockers include dry non-fat milk or serums like FBS, PBS, and BSA. For nitrocellulose membranes, which require...