We narrowed to 2,528 results for: pb

1. PB-EF1a-Puro-V8.2

   Pooled Library

   #178943

   Purpose

   Barcoding library for the macsGESTALT inducible lineage recording system

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Expression

   Mammalian

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

2. PB Neo* PB

   Plasmid

   #138413

   Purpose

   Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

   Depositor

   Mario Capecchi

   Insert

   Neo*
   

   Use

   Mouse Targeting; Piggybac

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS xenopus Endodermin (pBS-eddL6)

   Plasmid

   #24968

   Depositor

   Edward De Robertis

   Article

   Sasai et al EMBO J. 1996 Sep 2. 15(17):4547-55.

   Insert

   Endodermin (a2m Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-PEmax

   Plasmid

   #187893

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CRISPR

   Plasmid

   #160047

   Purpose

   For cloning sgRNA to knockout a specific gene in piggybac plasmid

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-HxB.L

   Plasmid

   #65414

   Purpose

   contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

   Depositor

   Harold Erickson

   Article

   Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

   Insert

   TNC large splice variant (TNC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-3xpA

   Plasmid

   #176505

   Purpose

   3x SV40 polyA sequences

   Depositor

   Mario Capecchi

   Insert

   3x SV40 polyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TO-hNGN2

   Plasmid

   #172115

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-Int

    Plasmid

    #50000

    Purpose

    Expresses the integrase on a suicide plasmid

    Depositor

    Peter Sander, Brian Robertson

    Article

    Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

    Insert

    Integrase from pMV361
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    own

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pBS-slpGFP

    Plasmid

    #27171

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-ldhGFP

    Plasmid

    #27170

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-U6

    Plasmid

    #120358

    Purpose

    piggyBac transposon vector for sgRNA expression.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-UniSAM

    Plasmid

    #99866

    Purpose

    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

    Depositor

    Lesley Forrester

    Article

    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

    Insert

    UniSAM-mCherry + U6-gRNA2.0
    

    Use

    CRISPR and Synthetic Biology; Dcas9-sam activation

    Tags

    Expression

    Mammalian

    Mutation

    BbsI sites in CDS were ablated by consensus mutat…

    Promoter

    EF1a

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-iCas9

    Plasmid

    #160048

    Purpose

    inducible expression of Cas9

    Depositor

    Xiaojun Lian

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-rtTA

    Plasmid

    #126034

    Purpose

    PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

    Depositor

    Mauro Calabrese

    Article

    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

    Insert

    rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TET

    Plasmid

    #20909

    Purpose

    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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