We narrowed to 25 results for: tfa
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TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....do you prepare for this shift and navigate the pitfalls? Here's what you need to know. Figure...new goals, which I’d like to share below. Common pitfalls in the transition (what I wish I knew before experimenting...
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Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing a bright...Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and cell biology 32.11... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m.... you had?). You can avoid some of these common pitfalls of management and still not get the most from ... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....veterans in the lab or the field? What are possible pitfalls in your strategy and how can you mitigate them... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....Manning CF, Bundros AM, Trimmer JS. Benefits and pitfalls of secondary antibodies: why choosing the right... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....shaving” would require multiple steps. One potential pitfall of REPLACR-mutagenesis is that you must PCR amplify... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....protein’s propensity to aggregate. The basics and pitfalls of these experiments are discussed here. In vitro... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins Control Protein... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....lists the deliverables, explains 1-2 potential pitfalls of the intended protocol, and offers alternative... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....zygote viability. Nanoblades, however, avoid this pitfall since they are injected into the space surrounding... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....requires some time for chromophore maturation, splitFAST displays rapid and reversible complementation.... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m...., A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies: Why Choosing the Right... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m.... protein’s cellular role. One way to avoid the pitfalls of overexpressed fluorescent protein fusions is... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....methods and useful image analysis software tools. Pitfalls, limitations, and specialized colocalization methods... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....multi-fasta file of the hg19 genome using Bedtools getFasta. These regions and their reverse complements were... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and how best to design... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....characterization for in vivo studies. One of the pitfalls of CRISPR/Cas9 genome editing is the potential... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....protein in E. coli. We will review the potential pitfalls of recombinant protein expression and some of ... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....important to follow some simple rules to avoid common pitfalls and to use it at its best. What are the Parameters... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m.... the edited cells in our hands, these combined pitfalls made it virtually impossible to find the 0.2-0.5%...