We narrowed to 30 results for: tfa

1. Genetic Code Expansion

   Type

   Collection

   ...Thomas Huber 177311 pRSF-G1TFAKRS M. archaeon N6-(trifluoroacetyl)-L-lysine (TFA-Lys) Bacterial TAG Thomas...

2. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   ...do you prepare for this shift and navigate the pitfalls? Here's what you need to know.   Figure...new goals, which I’d like to share below. Common pitfalls in the transition (what I wish I knew before experimenting...

3. Allen Institute for Cell Science Plasmid Collection

   Type

   Collection

   ...Costameres 159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids...

4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   ...imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing a bright...Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and cell biology 32.11...

5. Management for Scientists: What Makes a Good Manager Anyway?

   Type

   Blog Post

   ... you had?). You can avoid some of these common pitfalls of management and still not get the most from ...

6. Advice for Choosing a Research Project

   Type

   Blog Post

   ...veterans in the lab or the field? What are possible pitfalls in your strategy and how can you mitigate them...

7. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ...Manning CF, Bundros AM, Trimmer JS. Benefits and pitfalls of secondary antibodies: why choosing the right...

8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   ...shaving” would require multiple steps. One potential pitfall of REPLACR-mutagenesis is that you must PCR amplify...

9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...protein’s propensity to aggregate. The basics and pitfalls of these experiments are discussed here. In vitro...

10. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins Control Protein...

11. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    ...lists the deliverables, explains 1-2 potential pitfalls of the intended protocol, and offers alternative...

12. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ...zygote viability. Nanoblades, however, avoid this pitfall since they are injected into the space surrounding...

13. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...requires some time for chromophore maturation, splitFAST displays rapid and reversible complementation....

14. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    ..., A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies: Why Choosing the Right...

15. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    ... protein’s cellular role. One way to avoid the pitfalls of overexpressed fluorescent protein fusions is...

16. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    ...methods and useful image analysis software tools. Pitfalls, limitations, and specialized colocalization methods...

17. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    ...multi-fasta file of the hg19 genome using Bedtools getFasta. These regions and their reverse complements were...

18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    ...faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and how best to design...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ...characterization for in vivo studies. One of the pitfalls of CRISPR/Cas9 genome editing is the potential...

20. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    ...protein in E. coli. We will review the potential pitfalls of recombinant protein expression and some of ...