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... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources...applications of fluorescent proteins and to find plasmids for your next experiment. Browse Fluorescent Protein...interest by fusing it to a fluorescent protein. Michael Davidson Fluorescent Protein Collection Browse ...Learn More and Explore Addgene Blog: Fluorescent Proteins Fluorescent Protein Plasmid Kits Allen Institute...this guide to learn about the applications of fluorescent proteins and to find the plasmids that are available...Resources Addgene's repository contains thousands of fluorescent protein plasmids. Use this guide to learn more... collection of genes and markers tagged with fluorescent proteins as well as hundreds of empty backbones...

... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...Luciferase can be paired with a fluorescent protein acceptor for Bioluminescence Resonance Energy Transfer ...Calibration Standards Additional Resources Förster (or Fluorescence) Resonance Energy Transfer (FRET) is a process...binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules have been demonstrated...experiments. FRET efficiency can be measured using fluorescence microscopy or spectroscopy by exciting the donor...emission of the acceptor. Alternatively, the fluorescence lifetime (the time between excitation of the...a photon) of the donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET...

... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...Additional Resources Fluorescent biosensors are proteins designed so that their fluorescent properties are ...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET...orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Orange fluorescent calcium...raitometric (KIRIN1) fluorescence indicators of K + Genetically encoded fluorescent indicators for imaging...AMP) Yellow fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based ...

...Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson...Empty Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top...top Cyan Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald... mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top...top Orange Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...photoconvertible, and photoactivatable fluorescent proteins. Plasmid Description Industry Return to top Content...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...

... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins...pPAiRFP2-N1 Additional Resources Fluorescent Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization...photoactivatable fluorescent proteins. Plasmid...Near-Infrared Photoactivatable Additional Resources Fluorescence microscopy is a powerful tool to study proteins...presents unique challenges. Genetically-encoded fluorescent proteins most suitable for stable imaging in ...undesirable background signals. Many near-infrared fluorescent proteins are derived from bacterial phytochrome...phytochrome photoreceptors. Spectrally distinct fluorescent iRFP variants have been shown to have high brightness...

... Plasmid Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular ...protein to a red fluorescent protein like mCherry (find plasmids for cloning in our Fluorescent Proteins Empty...constructs or different fluorescent protein markers? Check out the Michael Davidson Fluorescent Protein Collection...Collection Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Content last ...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...of interest. One of the most common is to use fluorescence microscopy to compare its localization with ...Addgene has assembled a collection of popular fluorescent fusion proteins that are targeted to various ...

...Return to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones ...Maturation is the time for fluorescence to reach half its maximal value. Structure describes the oligomeric state...Botman-Teusink Yeast Fluorescent Protein Collection Plasmid Kits Hamdoun Lab Fluorescent Protein Fusion Construct...Addgene Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...Resources The following tables list many popular fluorescent proteins and empty backbones for expression and...

...ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter activity Fluorescence (mCherry...activity Fluorescence (mCherry) Escherichia coli Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2...activity Fluorescence (mRFP1) Escherichia coli Mary Dunlop 124605 pSCM001 Cytoplasmic pH Fluorescence (mCherry... Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein...Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic...pBAD Arabinose Escherichia coli Christian Raetz 26098 pCW-LIC 3xPtac Lactose/IPTG Escherichia coli Cheryl...Lactose/IPTG Escherichia coli Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas...

... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...

...popular reporter plasmids that feature fluorescent, luminescent, and color-producing proteins for detection...Resources Visit our Fluorescent Protein Guide for more plasmids containing fluorescent proteins. A set of...promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Synthetic Biology ...for creating plant expression vectors. ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning ...editing, dexamethasone-inducible expression, and fluorescent plasma membrane and nuclear markers. Golden Gate...different functional variants of Cas9. ID Plasmid Description PI Genome Editing Kits The following plasmid ...designed for genome editing in plants. ID Kit Description Gene Editing Type PI 1000000076 GoldenBraid 2.0...