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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 49 results for: Lif

Showing: 1 - 10 of 49 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...85054 pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella 85055 pLifeAct_mScarlet-H_N1 ...Filaments LifeAct mScarlet-H Dorus Gadella 85056 pLifeAct_mScarlet-i_N1 Actin Filaments LifeAct mScarlet-I...54491 mCherry-Lifeact-7 Actin Filaments LifeAct mCherry* Michael Davidson 54610 mEGFP-Lifeact-7 Actin Filaments...mCherry Christien Merrifield 36201 pLifeAct-mTurquoise2 Actin Filaments LifeAct mTurquoise2 Dorus Gadella 36202...EB1 GFP Robin Shaw 40908 pDEST/LIfeAct-mCherry-N1 Actin Filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin...miRFP703 Vladislav Verkhusha 79993 pLifeAct-miRFP703 Actin Filaments LifeAct miRFP703 Vladislav Verkhusha 79994...Filaments LifeAct EGFP Michael Davidson 55148 mCherry-Tubulin-C-18 Microtubules alpha-tubulin mCherry* ...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor for...for detecting signaling activities in live cells. eLife. 2018 Jul 3;7. Jin Zhang Calcium ER or mitochondria-targeted...calcium indicators for imaging neural activity. eLife. 2016 Mar 24;5. pii: e12727. Douglas Kim , GENIE...for detecting signaling activities in live cells. eLife. 2018 Jul 3;7. pii: 35458 Jin Zhang cAMP (cyclic...fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based Glucose Indicators...fluorescent glucose indicators for living cells (Glifons) Development of red genetically encoded biosensor...concentrations with a genetically encoded fluorescence lifetime sensor. J Neurosci Res. 2019 May 20. Gary Yellen...

  3. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S...expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1...expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22...expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1... Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2... Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana Gradinaru All Available AAV Packaging...

  4. Plan Your Experiment

    Type
    Collection
    ...cell population. The region of interest is PCR amplified, PCR products are denatured-renatured, treated...restriction site. The region of interest is PCR amplified, digested with the appropriate restriction enzyme... agarose gel to identify DNA fragments. PCR amplification and gel electrophoresis (for HDR or NHEJ): For...insertions, the region of interest can be PCR amplified using primers that (A) flank the region of interest...determine whether the edit was successful. PCR amplification, subcloning and Sanger sequencing (for HDR or... sequence of targeted alleles. Involves PCR amplification of targeted region from DNA, subcloning into...plasmid, and screening individual clones. PCR amplification and next-generation sequencing (for HDR or NHEJ...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...deletion band” is often too large to efficiently amplify. Typically use primers at least 100 bp from the...each sgRNA target site (within 150 - 350 bp) to amplify the sgRNA target site to examine for scarring. ...small deletions (as the “deletion band” may still amplify), resolve agarose gel to determine if size is consistent... incubation time allows for sufficient cell proliferation for screening genomic DNA (gDNA) for the intended... and 7.1 ). The bulk cells have sufficiently proliferated when the concentration exceeds ~100,000 cells...80% confluence. The clones have sufficiently proliferated once macroscopically visible with ~2 mm diameter...RNA and/or protein levels. To evaluate the DNA, amplify deletion bands from biallelic deletion clones with...

  6. CRISPR Guide

    Type
    Collection
    ...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by... by the depositing lab. After amplification, you’ll need to use next-generation sequencing (NGS) to verify...verify that the composition of your amplified library matches that of the original library. Next, you’... copies of the aptamer improves stability and amplifies the signal for better imaging of genomic loci,...SHERLOCK (and SHERLOCKv2) allows for greater amplification and quantification and can be used on lateral... Innovative Genomics Institute, University of California, Berkeley. Plan Your CRISPR Experiment Resources... DNA PCR P olymerase C hain R eaction; Used to amplify a specific sequence of DNA pegRNA P rime E dit ...

  7. mTOR Pathway

    Type
    Collection
    ... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...

  8. Antibody Guide

    Type
    Collection
    ...Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification Methods ...present on the Fc region. This allows for the amplification of signals by having multiple secondary antibodies...on the antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific ...region of primary antibodies allows for signal amplification through the indirect detection method. In the...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...

  9. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ....5, NR1C1, PPAR, PPARalpha, hPPAR peroxisome proliferator-activated receptor alpha PPARD Plasmids PPARD..., NR1C2, NUC1, NUCI, NUCII, PPARB peroxisome proliferator-activated receptor delta PPARG Plasmids PPARG... NR1C3, PPARG1, PPARG2, PPARgamma peroxisome proliferator-activated receptor gamma RARA Plasmids RARA,...

  10. AAV Packaged on Request

    Type
    Collection
    ...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...legal approval and an implementation letter DNA amplification Viral vector production Density centrifugation... Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive...
Showing: 1 - 10 of 49 results