Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 18 results for: c myc vectors

Showing: 1 - 18 of 18 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent...doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses... Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors for human...doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 Reprogramming ...doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 Excluding Oct4...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...state using a cocktail of factors (Oct3/4, Sox2, c-Myc, and Klf4) that are known to maintain pluripotency...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc tag pPMW-attB -...2NLS; pCDNA3 vector backbone, mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal 3x...pNIC-CTHF - C-terminal TEV-His6-Flag for bacterial expression pFA6a vectors - PCR-based C-terminal...in plants Myc Epitope tag pKMyc - N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's...Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep...LRRK2-WT LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted ... pGW1-Myc-DJ1-WT PARK7 V5 CMV Parkinson's Mark Cookson 29349 pGW1-Myc-DJ1-L166P/K130R PARK7 Myc CMV Parkinson's...D1994S)-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161585 pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc LRRK2...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc, p53, and MAPK/...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription... Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription...pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator under the c-Fos promoter Tet-On 3G ...opens in a new window) Krueger, C., Pfleiderer, K., Hillen, W., & Berens, C. (2004). Tetracycline derivatives...Lentiviral Tet-On vector for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced...Lentiviral Tet-On vector with blasticidin selection. See Plasmid #26730 for hygromycin selection. rtTA3...

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651 (Set D) Knockout Human Doench...TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon Human...Knockout Library 171531 Knockout Human Li 3rd ~6 9,274 MYC-CRISPR Library 173195 Knockout Human Dzikiewicz-Krawczyk...pneumoniae M. tuberculosis M. smegmatis Green monkey ( C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus...1000000074 (Puromycin) Activation Human Zhang 3rd 3 70,290 SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation...Library 159391 Knockout Mouse Moffat 3rd 4 182 Mycobacterium tuberculosis CRISPRi Library (RLC12) 163954 ...

  7. Bacterial Expression Systems

    Type
    Collection
    ...peptides include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr...selection of mammalian and bacterial FRET-related vectors and standards. The Hoschschild Bacterial Two-Hybrid...FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET biosensors for...#1000000222) is a collection of Golden Gate compatible vectors for bimolecular fluorescence complementation experiments...reconstructed) BiFC Lynne Regan 168257 168472 pMRBad-C-wtBlc pET11a-N-DiB2 DiB2 (reconstructed) BiFC Jens...coli Cynthia Collins 112197 pANY3 Temperature (42 °C) Escherichia coli Yingfeng An Other Addgene Controlled...Lu 17972 pSE100 Pmyc1/TetO Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Sabine...

  8. Viral Production

    Type
    Collection
    ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...produced the viral vector lot. Contact us to determine which facility produced your viral vector lot. At Addgene...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...-80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania Vector Core...80 °C. Titer All titering is performed on lentiviral preparations that have been stored at -80 °C and ... Viral Vector Packaging Service Viral Production Viral Production...

  9. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein DR274 42250 C. elegans BsaI none S. pyogenes...expression vectors for insertion of custom gRNA target sequences. CRISPR... CRISPR Empty gRNA Vectors CRISPR Plasmids...

  10. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...10.1016/j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...inducing a DNA break. Cytosine base editors convert C->T (or G->A on the opposite strand) within a small...which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter...NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9...

  11. CRISPR Plasmids - Plants

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...inducing a DNA break. Cytosine base editors convert C->T (or G->A on the opposite strand) within a small...which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene... As Cpf1 Qi 91715 pKEE401 yes, cut S. pyogenes Neomycin Chen Do you have suggestions for other plasmids...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... table below. Use the search bar to find a gRNA vector based on expression system, promoter, the type ...which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme ...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG...

  13. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...split", "rapamycin", “tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type...you can do with it, read Addgene's blog on FLEx Vectors . Flp-IN: A single FRT site within the genome of...to minimize leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below to...and filter the results by expression system and vector type. Find in-stock viral preps expressing Cre ...Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search...and filter the results by expression system and vector type. Find in-stock viral preps expressing Flp ...

  14. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Vickers, C. E., Bydder, S. F., Zhou, Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https:...

  15. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-...3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized...coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response...production, including codon optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which...expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab - Protocol and...

  16. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6...

  17. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated...Organism or species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic...

  18. CRISPR References and Information

    Type
    Collection
    ... design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish, mouse, rat, and ...
Showing: 1 - 18 of 18 results