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  1. Tetracycline Inducible Expression

    Type
    Collection
    ...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections...Bujard tested the tet system in a mammalian cell system (HeLa) and found that the tet system was functional...interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated Tet-On 3G System. Contains...Genomic Safe Harbor Locus. Tet-On 3G On Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system...LTR promoters ; see article for lentiviral vectors for tet-inducible gene or miRNA expression rtTA On ...Collections Background Tetracycline Off Tetracycline On Experimental Tips Tet Plasmids Background To advance the...

  2. AAV Molecular Tools

    Type
    Collection
    ...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...AAV Molecular Tools Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity...TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP 1...service encoding tet-off transactivators and tools for affinity purification (TRAP). Viral... Viral Vector Packaging ...pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1...Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... vector - Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral... bacterial expression vector (see tagged expression vectors from the SGC) pPro18... Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi...Backbones Neomycin (G418) Mammalian Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression...Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression...Lentiviral Gateway destination vector for gene expression (tet inducible) Zeocin/Bleo Mammalian...Zeo DEST - Tet-inducible lentiviral Gateway destination vector for gene expression...

  4. Mammalian RNAi Tools

    Type
    Collection
    ...expression Tyler Jacks Tet-Regulated Lentiviral shRNA Lentiviral vectors for Tet-regulated shRNA expression...shRNA cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should...Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative control vector for ...protocol for using the popular cloning vector pLKO.1. Trono Lab Lentivectors Toolbox Tyler Jacks Lab Protocols...Constitutive Lentiviral Cre-regulated Lentiviral Tet-regulated Lentiviral Constitutive Retroviral Resources... plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing...

  5. Lentivirus Plasmids

    Type
    Collection
    ...Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco...14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin...EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB...cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway... other versions of pSLIK. Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...See plasmid 21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin...

  6. Cre-lox system

    Type
    Collection
    ... pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre...promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...introns tet-hCMV Mammalian Sauer 12168 pMB80 (R26-CreER) Cre-ERT2 with loxp cassette; Targeting vector for...Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian Luo...Cre-ERT2;Targeting vector for Fos locus Mammalian Luo 46389 pArcCreER Cre-ERT2;Targeting vector for Arc locus...Burgess 61570 Pvalb-2A-Cre targeting vector Cre; Targeting vector for Pvalb locus Mammalian Zeng 61573...61573 Rasgrf2-2A-dCre targeting vector DHFR-destabilized Cre; targeting vector for Rasgrf2 Mammalian Zeng ...

  7. Plasmid Collections

    Type
    Collection
    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits are...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...

  8. Retrovirus Plasmids

    Type
    Collection
    ...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

  9. Neurodegeneration Research Collection

    Type
    Collection
    ...motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS... bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

  10. Plan Your Experiment

    Type
    Collection
    ...expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 ... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized... exact cloning strategy will depend on the gRNA vector you have chosen, so it is best to review the protocol...

  11. Brain Initiative Collection

    Type
    Collection
    ...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD expression. Bryan Roth...neuronal soma and proximal dendrites in a viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP...separated from nuclear mRuby3 by P2A in a viral vector Hillel Adesnik 109048-AAV9 pAAV-CAG-DIO-NLS-mRuby3... of internal ribosome entry sequence in a viral vector Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 ...Gradinaru 117383-AAV1 TRE-DIO-eYFP An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent...Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom AAV vector to drive the expression of dTomato in PV cortical...Dimidschstein 135630-AAV9 pAAV-S5E2-dTom-nlsdTom AAV vector to drive the expression of dTomato in PV cortical...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...expression vectors for insertion of custom gRNA target sequences. CRISPR... CRISPR Empty gRNA Vectors CRISPR Plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...

  13. Bacterial Expression Systems

    Type
    Collection
    ...expression vector containing N-terminal, TEV cleavable, 6x-His and thioredoxin tags. Other empty vectors for ...PIs Fluorescent protein fusion: contains empty vectors with N and C-terminal fluorescent protein tags .... pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression of an N-terminally... . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression of an N-terminally...6x His David Waugh Empty lactose/IPTG inducible vector for expression of a recombinant protein with an...periplasm David Waugh Empty lactose/IPTG inducible vector for expression of a recombinant protein with an...remove tag Nicola Burgess Brown pGEX expression vector with N-terminal GST tag and TEV cleavage site. ...

  14. Qi Lab CRISPR Page

    Type
    Collection
    .../ARS vector (Leu2) that contains dCas9 fused to N 51025 pSLQ1661-sgMUC4-E3(F+E) Lentiviral vector that...bacteria ) contains an anhydrotetracycline (aTc)-inducible dcas9 gene on a p15A vector with chloramphenicol...endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled...constitutive sgRNA expression cassette on a ColE1 vector with ampicillin resistance, wherein the N20 can...constructs 46910 pHR-SFFV-dCas9-BFP Human expression vector containing SFFV promoter, dCas9 that is fused to... 46911 pHR-SFFV-dCas9-BFP-KRAB Human expression vector containing SFFV promoter, dCas9 that is fused to...46912 pMSCV-LTR-dCas9-VP64-BFP Human expression vector containing MSCV LTR promoter, dCas9 that is fused...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...ZsGreen 493 505 39 Tetramer pHIV-Zsgreen - Mammalian Expression (this is a bicistronic vector rather than a... a fusion protein vector) ZsGreen1-N1 - Mammalian Expression SGFP2 495 512 30 Monomer (A206K) pSGFP2-C1...93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow Protein...5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine - Zebrafish...Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1...562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+8CmOrange... - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593 57 5.4...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding...Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...SpCas9 or SpCas9n, and the single guide RNA. The vector can be digested using BbsI, and a pair of annealed...indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed...gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous gene activation...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ...contains modified pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based ...UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI...Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing...RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate TALEN kit. The GoldyTALEN...mutation at rates much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from...pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN kit (Bogdanove/Voytas...the GAL1 promoter, placing TALORs built into this vector under galactose-inducible expression. pTAL6-BB ...

  18. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins for FRET The following...pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between ECFP...-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-5aa linker-Amber-6aa linker-Venus ACVA Heterotetrameric construct consisting of Amber-5aa linker-Cerulean...linker-Cerulean-5aa linker-Venus-6aa linker-Amber VCAA Heterotetrameric construct consisting of Venus-5aa linker-Cerulean...linker-Cerulean-5aa linker-Amber-6aa linker-Amber VCVV Heterotetrameric construct consisting of Venus-5aa linker-Cerulean...

  19. AAV Viral Preps

    Type
    Collection
    ... Viral Vector Packaging...Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively... in small volumes, and ready to ship. All viral vector preps are purified by density centrifugation. Addgene...or AAVrg). For more information, visit our Viral Vector Packaging Service overview. Function Chemogenetics...Serotype Testing Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, ...viral vectory packaging service. Browse constructs currently available in viral format. Viral...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell...

  20. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Empty gRNA Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search based...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...marker or cloning method. To isolate mammalian vectors, simply type "Mammalian" into the search bar. When...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single... by DNMT3A or MQ1, and cytosine demethylation by Tet1. These modifications persist over time and are potentially...

  21. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...interest 85586 gRNA Cloning Vector Bbs I ver. 2 An empty sgRNA expression vector. sgRNA sequence can be cloned...recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL...recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene 64325 3xFLAG-...p-bacteria Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify...

  22. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication Return to Top Other Sensors...sensors, ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian..., reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless ...reagentless biosensor for ADP based on tetramethylrhodamine-labeled ParM. ACS Chem Biol. 2010 Apr 16;5(4):415-25...

  23. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...10.1016/j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of specific genomic...

  24. Luciferase Plasmid Collection

    Type
    Collection
    ...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...interest are cloned into a Firefly luciferase reporter vector via Gateway cloning to test for the presence of...pGL4.84(hRlucCP/Puro) RapidResponse(TM) Renilla Vector for investigating regions controlling transcription...Pete Stecha 212933 pGL4.84(hRlucCP/Puro) Renilla Vector for investigating regions controlling transcription...expression of firefly luciferase Paul Khavari 109395 EFS-Vector Firefly EF1α AAV-CRISPR knockout plasmid. Firefly...moniter viral transfection Sidi Chen 109396 TBG-Vector Firefly TBG AAV-CRISPR knockout plasmid. Firefly...MLRV Multiple Multiple Multi-luciferase reporter vector including transcriptional reporters for NF-kb, ...

  25. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...methylation by DNMT3A or MQ1 cytosine demethylation by Tet1 These modifications persist over time and are potentially...

  26. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ... 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1 XX Amp) Plasmid 68300 MBP-MEK1 Plasmid...

  27. Genetic Code Expansion

    Type
    Collection
    ...amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI...Ryan Mehl 85496 pDule-Tet2.0 Tetrazine2.0 tRNA synthetase M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan...Ryan Mehl 85497 pDule2-Tet2.0 Tetrazine2.0 tRNA synthetas M. jannaschii Tetrazine 2.0 Bacterial TAG Ryan...Bacterial TAG Ryan Mehl 164580 pUltraI-Tet3.0[TAA] Tet3.0RS M. barkeri Tet3.0 Bacterial TAA Ryan Mehl 172482 ...TAG Ryan Mehl 174080 pDule-Tet3.0 Tet3.0 tRNA synthetase M. barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl...NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84 tRNA synthetase M. barkeri Tetrazine 3.0 Mammalian Ryan...aminoacyl-tRNA synthetase M. jannaschii 1,2,4,5-tetrazine ncAAs Bacterial TAG Ryan Mehl 217361 pIDTSmart-MbPylRS...

  28. CRISPR Guide

    Type
    Collection
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual... included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with a ...based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID: ...

  29. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods.... PubMed (Link opens in a new window) Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population...

  30. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...Ataxia Richard Youle 176485 Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius Wernig 176852 UBQLN2...Ronald Hart 190811 pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro... CMV Ataxia telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's John Gearhart ...T2A-Puro APOE UbC Alzheimer's Ronald Hart 190813 pTet-O-APOE-E4-T2A-Puro APOE UbC Alzheimer's Ronald Hart ...
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